Enzyme catalysis in ionic liquids: lipase catalysed kinetic resolution of 1-phenylethanol with improved enantioselectivity

Abstract

Lipases show good activity and, in some cases, improved enantioselectivity when employed in pure ionic liquids for dynamic kinetic resolution of 1-phenylethanol by transesterification.

Activation of lipase in ionic liquids by modification with comb-shaped poly(ethylene glycol)

“…3. This result is in agreement with those from previous reports [11,12]. This loss of activity would be caused by the insolubility of the biocatalyst or by saltinduced inactivation of the lipase.…”

“…Since the pioneering studies by some groups [8][9][10], many biocatalytic reactions in ILs have been actively conducted with lipases [11][12][13][14], proteases [15,16], oxidoreductases [17,18], and even with a whole-cell biocatalyst [19]. When compared to reactions performed in conventional organic solvents, ILs provide a more suitable environment for enzymes [16,20] and are obviously less harmful to cell membranes [19], resulting in high catalytic performance.…”

Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

“…Furthermore, the application of the immobilized PPL was extended to screening for lipase inhibitors with two natural products isolated from oolong tea (i.e., EGCG and EGC) and orlistat, a well-known lipase inhibitor. To date, the immobilized PPL was mainly limited to biocatalyzation in kinetic resolution of highly diverse racemic mixtures for the products of industry, ester synthesis and esterification conversion in the organic synthesis [14][15][16][17], and this is the first report that the magnetically separable biocatalysts be applied in the screening of lipase inhibitors.…”

Ionic liquids in biotechnology: applications and perspectives for biotransformations

“…For the transesterification of 1-phenylethanol with vinyl acetate, eight different enzymes in ten different IL were investigated (Schöfer et al 2001). For all but one lipase, best results were obtained in IL.…”