Enzyme-conjugated ZIF-8 particles as efficient and stable Pickering interfacial biocatalysts for biphasic biocatalysis

Shi, Jiafu; Wang, Xiaoli; Zhang, Shaohua; Tang, Lei; Jiang, Zhongyi

doi:10.1039/c6tb00104a

Cited by 104 publications

(63 citation statements)

References 40 publications

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“…Covalently linking enzyme molecules on the surface of MOF particles (Shi et al 2016; Cao et al 2016) was reported as another effective way for enzyme immobilization on MOFs, where the enzyme molecules were conjugated to the carboxylate groups on the MOFs. Co-precipitation or biomimetic mineralization (Lyu et al 2014; Li et al 2016a, b; Liang et al 2015a, b, 2016b, c; Wu et al 2015a, b; He et al 2016; Shieh et al 2015), a self-assembly process to encapsulate bioactive molecules within the protective exteriors, has been successfully introduced to the synthesis of enzyme-MOF composites.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents

Yang

2017

Bioresour. Bioprocess.

139

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ObjectivesEnzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study.ResultsEncapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs. Remarkably, when exposed to protein denaturing solvents including dimethyl sulfoxide, dimethyl formamide, methanol, and ethanol, the enzyme/MOF composites still preserved almost 100% of activity. In contrast, free enzymes retained no more than 20% of their original activities at the same condition. This study shows the extraordinary protecting effect of MOF shell on increasing enzyme stability at extremely harsh conditions.ConclusionThe enzyme immobilized in MOF exhibited enhanced thermal stability and high tolerance towards protein denaturing organic solvents.Electronic supplementary materialThe online version of this article (doi:10.1186/s40643-017-0154-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents

Yang

2017

Bioresour. Bioprocess.

139

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“…Shi et al. immobilized the lipase from Candida rugosa on ZIF‐8 synthesized by a solvothermal method by using methanol as the solvent . Liang et al.…”

Section: Introductionmentioning

confidence: 99%

Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium

et al. 2018

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Lipase AK from Pseudomonas fluorescens and Lipase RM from Rhizomucor miehei were encapsulated into a zeolite imidazolate framework (ZIF‐8) by a “one‐pot” synthesis to obtain AK@ZIF‐8 and RM@ZIF‐8 biocatalysts. The effect of a high (1:40) and low (1:4) Zn/2‐methylimidazole molar ratio on the biocatalysts synthesis was investigated. The different Zn/ligand (L) ratios affected both the surface area, the loading, and the specific activity of the biocatalysts. Samples synthesized by using a high Zn/L ratio had high values of surface area whereas those obtained by using a low Zn/L ratio had higher loadings and specific activities. The decrease of pH (from 11.6 to 9.4) during the synthesis at high Zn/L ratio produced ZIF‐8 samples with features similar to those observed for low Zn/L ratio samples. The low Zn/L (1:4) ratio AK@ZIF‐8 biocatalyst retained 99 % activity after storage for 15 days at 5 °C and 40 % activity after five reaction cycles.

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“…[2] To address this limitation, Pickering interfacial catalysis (PIC) has been developed. [1b] Fore xample,l ipases were attached into/onto metal-organic frameworks (MOFs), [8] polymersomes, [9] and Janus silica [10] for both esterification and hydrolysis reactions at the water-oil interface.P IB promotes not only reaction efficiency but also biocatalyst recyclability and usability after operation. [1b] Fore xample,l ipases were attached into/onto metal-organic frameworks (MOFs), [8] polymersomes, [9] and Janus silica [10] for both esterification and hydrolysis reactions at the water-oil interface.P IB promotes not only reaction efficiency but also biocatalyst recyclability and usability after operation.…”

mentioning

confidence: 99%

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions

et al. 2018

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Despite the rapid development of Pickering interfacial catalysis (PIC) at liquid-liquid interfaces with chemocatalysts,the use of unstable biocatalysts at emulsion interfaces remains at echnical challenge.H erein, we present aP ickering interfacial biocatalysis (PIB) platform based on robust and recyclable enzyme-polymer conjugates that act as both catalytic sites and stabilizers at the interface of Pickering emulsions.T he conjugates were prepared by growing poly(Nisopropylacrylamide) on afragile enzyme,benzaldehyde lyase, under physiological conditions.T he mild in situ conjugation process preserved the enzyme structure,a nd the conjugates were used to emulsify aw ater-organic two-phase system into as table Pickering emulsion, leading to as ignificantly larger interfacial area and a2 70-fold improvement in catalytic performance as compared to the unemulsified two-phase system. The PIB system could be reused multiple times. Conjugates of other enzymes were also fabricated and applied for cascade reactions.

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Enzyme-conjugated ZIF-8 particles as efficient and stable Pickering interfacial biocatalysts for biphasic biocatalysis

Abstract: Enzyme-based biphasic catalytic reactions were successfully accomplished by utilizing CRL-conjugated ZIF-8 particles as robust Pickering interfacial biocatalysts.

Cited by 104 publications

References 40 publications

Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents

Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents

Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions

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