1982
DOI: 10.1267/ahc.15.338
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Enzyme cytochemical study of rat cardiac muscle II. Ca++-ATPase and ouabain-sensitive, K+-dependent p-nitrophenylphosphatase.

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Cited by 29 publications
(9 citation statements)
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“…Ca++-ATPase activity, an energy dependent calcium extrusion pump, is well accepted to reflect intracellular calcium level. Some cytochemical investigations of the Ca++-ATPase in several organs have been reported with a new one-step method by Ando et al (1,2,9,10,23,28). In our present study with the use of this method the enzyme activity in the rat epidermis was detected.…”
supporting
confidence: 49%
“…Ca++-ATPase activity, an energy dependent calcium extrusion pump, is well accepted to reflect intracellular calcium level. Some cytochemical investigations of the Ca++-ATPase in several organs have been reported with a new one-step method by Ando et al (1,2,9,10,23,28). In our present study with the use of this method the enzyme activity in the rat epidermis was detected.…”
supporting
confidence: 49%
“…Sections were incubated at 37掳C for 30 minute for succinatic dehydrogenase (SDH) activity (Novikoff et al, 1961) and mitochondrial Ca-adenosine triphosphatase (mitochondrial Ca-ATPase) activity (Fujimoto and Ogawa, 1982). Under a microscope, an ocular micrometer of 100pm on each side was placed at the intersection of X-Y axes.…”
Section: Methodsmentioning
confidence: 99%
“…Serial cross sections (about 101.1m) were cut in a cryostat at-20'C, and air dried. Each section was incubated for Ca-activated adenosine triphosphatase at pH 9.4 (Padykula and Herman, 1955), after preincubation at various pH levels (pH 9.4, 5.0, 4.7, 4.5 or 4.3), for succinic dehydrogenase (SDH) activity (Novikoff et al, 1961), and for Ca-adenosine triphosphatase (mitochondrial Ca-ATPase) activity (Fujimoto and Ogawa, 1982), Sudan black B stain (Gauthier and Padykula, 1966) and glycerophosphate dehydrogenase (a-GPDH) (Pearse, 1972), and periodic acid-Schiff reaction (PAS) were used to demonstrate the types of jaw muscle fibers. According to system of Putnam et al, (1980), fibers were classified into four types, as fast twitch glycolytic (FG), fast twitch oxidative glycolytic (FOG), slow twitch oxidative (SO) fibers, and tonic fibers.…”
Section: Methodsmentioning
confidence: 99%