Enzyme Engineering: Current Trends and Future Perspectives

Sharma, Anshul; Gupta, Gaganjot; Ahmad, Tawseef; Mansoor, Sheikh; Kaur, Baljinder

doi:10.1080/87559129.2019.1695835

Cited by 111 publications

(62 citation statements)

References 135 publications

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“…Protein engineering is another relevant strategy to enhance stability [4,77,78] (Figure 5). Rational design takes benefit of structures and sequences of already known stable proteins.…”

Section: Enzyme Engineeringmentioning

confidence: 99%

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

et al. 2021

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Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing, electricity production, or biosynthesis of fine products. Despite advances in the knowledge of parameters that drive the efficiency of enzymatic electrocatalysis, the weak stability of bioelectrodes prevents large scale development of bioelectrocatalysis. In this review, starting from the understanding of the parameters that drive protein instability, we will discuss the main strategies available to improve all enzyme stability, including use of chemicals, protein engineering and immobilization. Considering in a second step the additional requirements for use of redox enzymes, we will evaluate how far these general strategies can be applied to bioelectrocatalysis.

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“…Protein engineering is another relevant strategy to enhance stability [4,77,78] (Figure 5). Rational design takes benefit of structures and sequences of already known stable proteins.…”

Section: Enzyme Engineeringmentioning

confidence: 99%

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

et al. 2021

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“…Thus, diverse enzyme engineering approaches to improve catalytic, stability and productivity properties have been developed. Ultimately, the goal of enzymatic genetic engineering is to modify the amino acid sequences through gene alteration [ 60 ].…”

Section: Introductionmentioning

confidence: 99%

“…In directed evolution, no data on the protein structure and function are required, and it works by building a library of randomly generated mutants. On the other hand, in rational enzymatic design or semi-rational design, information on protein structure and function is required [ 60 , 61 ].…”

Section: Introductionmentioning

confidence: 99%

“…Enzymatic engineering through rational design, which implies introducing specific and pre-defined changes in the amino acid sequence by site-directed mutagenesis, has been growing as an emerging family of engineering techniques [ 60 , 62 ].…”

Section: Introductionmentioning

confidence: 99%

“…Since rational design implies knowledge of protein function and structure, it frequently begins with computer aided design [ 60 ]. The rapid increase of high-resolution protein structures available on the PDB database [ 63 ], and the improvement of structure-prediction tools, such as the recently released AlphaFold [ 64 ], allows for molecular and atomistic perspectives on the structure and function of enzymes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET

Magalhães

Cunha

Sousa

2021

IJMS

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Plastics are highly durable and widely used materials. Current methodologies of plastic degradation, elimination, and recycling are flawed. In recent years, biodegradation (the usage of microorganisms for material recycling) has grown as a valid alternative to previously used methods. The evolution of bioengineering techniques and the discovery of novel microorganisms and enzymes with degradation ability have been key. One of the most produced plastics is PET, a long chain polymer of terephthalic acid (TPA) and ethylene glycol (EG) repeating monomers. Many enzymes with PET degradation activity have been discovered, characterized, and engineered in the last few years. However, classification and integrated knowledge of these enzymes are not trivial. Therefore, in this work we present a summary of currently known PET degrading enzymes, focusing on their structural and activity characteristics, and summarizing engineering efforts to improve activity. Although several high potential enzymes have been discovered, further efforts to improve activity and thermal stability are necessary.

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