Enzyme-free colorimetric nanosensor for the rapid detection of lactic acid in food quality analysis

Rattu, Gurdeep; Krishna, P. Murali

doi:10.1016/j.jafr.2022.100268

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…In terms of H 2 O 2 detection, the LOD in our study is similar with other values reported in the literature. For lactic acid detection, our LOD matches that of Rattu but remains higher than that of Zhang . However, factors other than the LOD must be considered.…”

Section: Resultsmentioning

confidence: 99%

Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Pereira,

dos Santos,

Andre

et al. 2024

ACS Appl. Nano Mater.

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Milk and its derivatives are essential sources of nutrients for humans. However, the widespread adulteration of milk poses a significant challenge for ensuring the safety and integrity of these products. Here, a zein/manganese dioxide nanosheet (MnO 2 NS) composite-based colorimetric sensor integrated with a smartphone platform was developed for on-site and quantitative analysis of hydrogen peroxide (H 2 O 2 ) and lactic acid, two common adulterants in milk samples. Sensing platforms were fabricated by spraying composite solutions onto various costeffective and environmentally friendly substrates, including filter paper and masking tape, using a simple airbrush system. These platforms displayed remarkable sensitivity and suitable limit of detection (LOD) of 7.2 × 10 −4 mol L −1 for H 2 O 2 and 7.5 × 10 −4 mol L −1 for lactic acid, respectively. These concentrations fall within the ranges established by diverse international regulatory bodies responsible for quality assurance protocols. Therefore, the dual detection of H 2 O 2 and lactic acid in milk samples was successfully achieved in a rapid and cost-effective way, which opens possibilities for the design and implementation of field-deployable sensors for food safety surveillance and quality control.

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Section: Resultsmentioning

confidence: 99%

Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Pereira,

dos Santos,

Andre

et al. 2024

ACS Appl. Nano Mater.

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“…To assess the intensity of exercise under anaerobic metabolic conditions and prevent metabolic acidosis and fatigue from excessive exercise, lactate works as an essential biomarker among the numerous metabolites within the human body. , In recent years, numerous analytical techniques for lactate detection have been developed, such as fluorometry, colorimetry, nuclear magnetic resonance, gas chromatography, and high-performance liquid chromatography. − However, the major challenges are complex lengthy pretreatment processes, high costs, and low efficiency . When compared to other techniques, the electrochemistry technique is the preffered method for sweat monitoring due to its ability to provide real-time analysis, potential for miniaturization, relatively high sensitivity, and rapid responsiveness .…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Graphene Quantum Dots Incorporated into MOF-Derived NiCo Layered Double Hydroxides for Nonenzymatic Lactate Detection in Noninvasive Biosensors

Chang,

Rinawati,

Guo

et al. 2024

ACS Appl. Nano Mater.

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Rapid interest in identifying specific biomarkers has been sparked by the development of wearable electrochemical sensors for physiological and biological monitoring via noninvasive measurement. During anaerobic metabolic circumstances, monitoring the lactate content become critical for noninvasive diagnostic of hypoxia. To improve the sensitivity of wearable sweat biosensors for detecting lactate concentrations, in this study, metal−organic framework (MOF)-derived NiCo-based layered double hydroxides (m-NiCo LDHs) with N-doped graphene quantum dots (NGQDs) decoration are designed. According to the X-ray absorption spectroscopy (XAS) analysis, the incorporation of NGQDs will alter the local electronic structure of transition metals in m-NiCo LDHs, thereby reducing the charge transfer resistance and accelerating the electron transfer kinetics during electrochemical reactions of lactate detection. After understanding the role of NGQDs in the matrix of m-NiCo LDHs, as-designed NGQD/m-NiCo LDH-based electrochemical biosensors for lactate detection displayed superior sensitivity of 62.63 ± 1.50 μA mM −1 cm −2 under an applied potential of 0.60 V (vs Ag/AgCl/3 M KCl) with the lactate concentration range of 0 to 15 mM in alkaline condition, compared to pristine NiCo LDH (16.77 ± 1.70 μA mM −1 cm −2 )-and m-NiCo LDH (45.45 ± 4.39 μA mM −1 cm −2 )-based ones.This research provides a potential electrocatalyst of GQD-modified MOF-derived LDHs for using enzyme-free electrochemical lactate sensors with reliable and stable performance in order to implement noninvasive human perspiration monitoring on wearable bioelectronics.

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“…For Lact analysis, many optic and electrochemical methods have been proposed [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. Most of these methods are nonselective, time-consuming, costly, need pretreatment, and require expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

“…Nanomaterials in the bio-recognition layer of the ABS serve as a matrix for enzyme immobilization and mediate electron transfer from the enzyme to the electrode surface. Nanoparticles (NPs) of metal oxides, metals, semiconductors, and composite NPs or nanohybrids perform various functions in electrochemical conversion circuits; in particular, much attention is paid to their mediator properties [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. To improve electronic exchange between the surface of the working electrode and the enzyme in the planned ABS, a search for optimal nanomediators was performed.…”

Section: Introductionmentioning

confidence: 99%

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

et al. 2023

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L-Lactate is an indicator of food quality, so its monitoring is essential. Enzymes of L-Lactate metabolism are promising tools for this aim. We describe here some highly sensitive biosensors for L-Lactate determination which were developed using flavocytochrome b2 (Fcb2) as a bio-recognition element, and electroactive nanoparticles (NPs) for enzyme immobilization. The enzyme was isolated from cells of the thermotolerant yeast Ogataea polymorpha. The possibility of direct electron transfer from the reduced form of Fcb2 to graphite electrodes has been confirmed, and the amplification of the electrochemical communication between the immobilized Fcb2 and the electrode surface was demonstrated to be achieved using redox nanomediators, both bound and freely diffusing. The fabricated biosensors exhibited high sensitivity (up to 1436 A·M−1·m−2), fast responses, and low limits of detection. One of the most effective biosensors, which contained co-immobilized Fcb2 and the hexacyanoferrate of gold, having a sensitivity of 253 A·M−1·m−2 without freely diffusing redox mediators, was used for L-Lactate analysis in samples of yogurts. A high correlation was observed between the values of analyte content determined using the biosensor and referenced enzymatic-chemical photometric methods. The developed biosensors based on Fcb2-mediated electroactive nanoparticles can be promising for applications in laboratories of food control.

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Enzyme-free colorimetric nanosensor for the rapid detection of lactic acid in food quality analysis

Cited by 5 publications

References 38 publications

Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Nitrogen-Doped Graphene Quantum Dots Incorporated into MOF-Derived NiCo Layered Double Hydroxides for Nonenzymatic Lactate Detection in Noninvasive Biosensors

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

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Enzyme-free colorimetric nanosensor for the rapid detection of lactic acid in food quality analysis

Cited by 5 publications

References 38 publications

Zein/MnO2 Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Zein/MnO2 Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Nitrogen-Doped Graphene Quantum Dots Incorporated into MOF-Derived NiCo Layered Double Hydroxides for Nonenzymatic Lactate Detection in Noninvasive Biosensors

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

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Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk

Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk