Enzyme immobilization on a 3D-printed reactor for aldehyde oxidation to carboxylic acid under mild conditions

Abstract

An aldehyde dehydrogenase from Geotrichum candidum catalyzing aldehyde oxidation was immobilized on a functionalized surface of a 3D-printed polypropylene reactor. The immobilization improved its thermostability. Furthermore, the immobilized enzyme could...

Ultrafast Solid-Phase Oxidation of Aldehydes to Carboxylic Acids by Atmosphseric Plasma Treatment

“…Despite the development of improved metal-catalyzed synthetic methods from the past decade, − more sustainable and efficient catalyst ligand- and metal-free strategies for aerobic oxidation are highly desired, especially the ones using only a small amount of solvent to reduce the environmental impact. , Apart from the triviality of this chemical reaction, many efforts were made, even very recently, to overcome the drawbacks and to tailor novel methods for today’s requirements. These recent methods are mainly designed alongside sustainability and green chemistry-oriented considerations. − However, many of them still suffer from the need of additional catalysts, − even if these catalysts are considered environmentally friendly ones.…”

“…These recent methods are mainly designed alongside sustainability and green chemistry-oriented considerations. 49 − 55 However, many of them still suffer from the need of additional catalysts, 49 − 55 even if these catalysts are considered environmentally friendly ones.…”

Facile Multilayer Deposition of Polyethylenimine and κ-Carrageenan on a 3D-Printed Lattice for Cellulase Immobilization

“…3D printing, or additive manufacturing, has recently come to light as a facile bottom-up manufacturing strategy that allows the swift prototyping and manufacturing of complex geometries . Several sophisticated continuous flow (CF) reactor designs have been successfully fabricated via 3D printing, such as the development of microreactors that incorporate enzyme immobilization and microfluidic technology and modular scaffolding structures that can be assembled as an independent enzymatic reactor or as a filler into an existing enzymatic reactor like the stirred tank reactor. − Cellulase was previously immobilized on a microreactor made of a polytetrafluoroethylene (PTFE) capillary column with the codeposition of dopamine (DA) and polyethylenimine (PEI). The glucose production from this microreactor was enhanced by up to 97.2% in Turnover Frequency (TOF) compared to cellulase immobilized on nanoparticles, showcasing better throughputs via the swift passive mixing by the flowing action of the substrate. , On the other hand, including lattices in a reactor offers improved surface area-to-volume ratio, facilitates enzyme recovery, and eases enzyme–product separation.…”