Enzyme immobilization on ZIF-67/MWCNT composite engenders high sensitivity electrochemical sensing

Liu, Xuejiao; Chen, Wanwan; Lian, Meiling; Chen, Xu; Lu, Yanluo; Yang, Wensheng

doi:10.1016/j.jelechem.2018.12.027

Cited by 74 publications

(28 citation statements)

References 45 publications

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“…[28] As well as for colorimetric sensing,r obust enzyme@MOFs biocomposites were also proposed to fabricate ultra-stable modified-electrode for electrochemical biosensing. [33] Furthermore,detecting disease biomarkers is critical for clinical diagnosis and the subsequent effective therapeutics.Aportable and robust enzyme@-MOFs-based on-paper biosensor enabling point-of-care diagnosis is undergoing in our group.T he designed new paperbased biosensor enables to analyze on-site different important disease-related biomolecules through modulating the enzyme cascades,c ombining with ac ustom-designed smartphone for signal readout.…”

Section: Angewandte Chemiementioning

confidence: 99%

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

et al. 2020

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Cell‐free enzymatic catalysis (CFEC) is an emerging biotechnology that enable the biological transformations in complex natural networks to be imitated. This biomimetic approach allows industrial products such as biofuels and biochemical to be manufactured in a green manner. Nevertheless, the main challenge in CFEC is the poor stability, which restricts the effectiveness and lifetime of enzymes in sophisticated applications. Immobilization of the enzymes within solid carriers is considered an efficient strategy for addressing these obstacles. Specifically, putting an “armor‐like” porous metal–organic framework (MOF) exoskeleton tightly around the enzymes not only shields the enzymes against external stimulus, but also allows the selective transport of guests through the accessible porous network. Herein we present the concept of this biotechnology of MOF‐entrapped enzymes and its cutting‐edge applications.

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Section: Angewandte Chemiementioning

confidence: 99%

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

et al. 2020

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“…Unfortunately, the absorption efficacy was low because of the hydrophobic property of the electrospun PCL fiber mat. A previous study indicated that the absorption efficiency of the enzyme could be remarkably enhanced with an increase in hydrophilicity [ 26 , 27 ]. Therefore, hydrophilic modification of the PCL fiber mat might be a proper way to improve the detection performance of the designed detection card.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Nano/Micro-Structured Electrospun Detection Card for the Detection of Pesticide Residues

Feng

Zhai

Wei

et al. 2021

Foods

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A novel nano/micro-structured pesticide detection card was developed by combining electrospinning and hydrophilic modification, and its feasibility for detecting different pesticides was investigated. Here, the plain and hydrophilic-modified poly(ε-caprolactone) (PCL) fiber mats were used for the absorption of indolyl acetate and acetylcholinesterase (AChE), respectively. By pre-treating the fiber mat with ethanol, its surface wettability was improved, thus, promoting the hydrolysis of the PCL fiber mat. Furthermore, the absorption efficiency of AChE was improved by almost two times due to the increased hydrophilicity of the modified fiber mat. Noteworthily, this self-made detection card showed a 5-fold, 2-fold, and 1.5-fold reduction of the minimum detectable concentration for carbofuran, malathion, and trichlorfon, respectively, compared to the national standard values. Additionally, it also exhibited good stability when stored at 4 °C and room temperature. The food detection test showed that this nano/micro-based detection card had better detectability than the commercial detection card. Therefore, this study offers new insights into the design of pesticide detection cards, which also broadens the application of electrospinning technique.

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“…[28] Abgesehen von kolorimetrischen Sensoren wurde vorgeschlagen, Enzym@MOF-Biokomposite auch als ultrastabile modifizierte Elektroden füre lektrochemische Biosensoren zu verwenden. [33] Ferner sind Detektoren fürK rankheitsbiomarker außerordentlich wichtig fürd ie medizinische Diagnostik und anschließende Therapie.W eitere Untersuchungen an tragbaren, robusten Enzym@MOF-Biosensor-Teststreifen fürd ie patientennahe Diagnostik werden gegenwärtig in unserer Arbeitsgruppe durchgeführt. Die entwickelten Biosensorteststreifen ermçglichen eine Vor-Ort-Analyse verschiedener wichtiger,k rankheitsrelevanter Biomoleküle durch die Modulierung der Enzymkaskaden, wobei ein speziell angepasstes Smartphone zum Auslesen des Signals verwendet wird.…”

Section: Sensorikunclassified

“Panzerung” von Enzymen mit Metall‐organischen Gerüsten

et al. 2020

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Zellfreie enzymatische Katalyse (CFEC) ist eine aufstrebende biotechnologische Methode, die es ermöglicht, biologische Umwandlungen in komplexen natürlichen Netzwerken zu imitieren. Dieser biomimetische Ansatz erlaubt es, Industrieprodukte wie Biokraftstoffe und biochemische Erzeugnisse auf umweltverträgliche Weise zu produzieren. Die größte Herausforderung bei der CFEC ist allerdings die Instabilität von Enzymen, die deren Effektivität und Lebensdauer in anspruchsvollen Anwendungen einschränkt. Die Immobilisierung von Enzymen in Festkörpern gilt als effiziente Strategie, um die zuvor beschriebenen Schwierigkeiten zu überwinden. Speziell die Entwicklung und Verwendung von rüstungsartigen, porösen Metall‐organischen Gerüsten (MOFs), welche die Enzyme exoskelettartig umschließen können, schützt die Enzyme nicht nur vor äußeren Einflüssen, sondern ermöglicht zusätzlich den Transport von Gastmolekülen über das poröse Netzwerk. Der vorliegende Kurzaufsatz stellt dieses biotechnologische Konzept, MOF‐umschlossene Enzyme und deren innovative Anwendungen vor.

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Enzyme immobilization on ZIF-67/MWCNT composite engenders high sensitivity electrochemical sensing

Cited by 74 publications

References 45 publications

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

Fabrication of Nano/Micro-Structured Electrospun Detection Card for the Detection of Pesticide Residues

“Panzerung” von Enzymen mit Metall‐organischen Gerüsten

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