Enzyme Immunoassay: A Review

O’Sullivan, Michael; Bridges, James W.; Marks, Vincent

doi:10.1177/000456327901600162

Cited by 110 publications

(25 citation statements)

References 137 publications

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“…fluorescence quenchers, chemiluminescence precursors) have been investigated and developed in the last years (Schall and Tenoso, 1981;Oellerich, 1980). In particular, enzyme immunoassays (Borrebaeck and Mattiasson, 1979;O'Sullivan et al, 1979;Engvall and Ruoslahti, 1979; Scharpe el af., 1976; Ngo and Lenhoff, 1981;Marks, 1981) have proved to be suitable alternatives to radioimmunoassays. The various ways in which enzymes have been employed.…”

Section: Introductionmentioning

confidence: 99%

Luciferin derivatives in bioluminescence‐enhanced enzyme immunoassays

Miska¹,

Geiger

1989

J. Biolumin. Chemilumin.

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Ultrasensitive bioluminescence immunoassays for the determination of peptides and proteins (illustrated with human urinary kallikrein, bradykinin and the determination of human urinary kallikrein antibody titres) have been developed. The usable ranges of the standard curves are from 5 pg to 5000 pg per litre. The relative intra-assay coefficients of variation of the tests were between 2% and 6%, and the inter-assay coefficients of variation between 4% and 12%.

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Section: Introductionmentioning

confidence: 99%

Luciferin derivatives in bioluminescence‐enhanced enzyme immunoassays

Miska¹,

Geiger

1989

J. Biolumin. Chemilumin.

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“…11α-hydroxyprogesterone-hemisuccinate BSA (molar ratio 15:1) was determined using trinitrobenzene sulfonate method (Satake, 1960). The 11α-hydroxyprogesterone-hemisuccinatehorseradish peroxidase conjugate used as labeled antigen in the competitive ELISA was prepared by the carbodiimide method (Gross and Bilk, 1968;O'Sullivan et al, 1979). 11α-hydroxyprogesteronehemisuccinate (2.46 µmol) was dissolved in 30 µl DMF, mixed with equal amounts of NHS and DCC, and the mixture was stirred for 12 h at room temperature.…”

Section: Standardsmentioning

confidence: 99%

Development of an ELISA-based kit for the on-farm determination of progesterone in milk

Simerský¹,

Swaczynová²,

Morris³

et al. 2007

Vet. Med. - Czech

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ABSTRACT:Effective reproductive management is a crucial concern in the dairy industry and missed oestrus and late recognition of unsuccessful insemination can lead to substantial financial loss. Progesterone levels change in a predictable manner with progress of the oestrus cycle and with the onset of pregnancy. We report here the development of a simple ELISA test for the measurement of progesterone levels in bovine milk. The method is based on the use of polyclonal antibodies raised against 11α-hydroxyprogesterone-hemisuccinate and competition for antibody binding sites between free progesterone and horseradish peroxidase-labelled progesterone. Binding is quantified by means of the colour reaction between horseradish peroxidase and 3,3' ,5,5'-tetramethylbenzidine. The method has proved to be a cost effective, sensitive and easy to perform alternative to currently available methods of oestrus and pregnancy detection. The practical feasibility of the method has been proven under dairy farm conditions.

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“…An alternative method is the enzyme-linked immunosorbent assay (ELISA). After the introduction of the ELISA for the determination of rabbit IgG (2), this technique has now become used in a large and still increasing number of applications which include measurements of antigens, haptens and antibodies (3,4).…”

Section: Introductionmentioning

confidence: 99%

Use of the Enzyme-Linked Immunosorbent Assay (ELISA) in Immunotoxicity Testing

Vos¹,

Krajnc²,

Beekhof³

1982

Environmental Health Perspectives

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Based on an earlier described macromethod for the routine measurement of IgM and IgG in rat sera, a mechanized micro enzyme-linked immunosorbent assay (ELISA) was developed. The assay was performed in the wells of microtiter plates thus minimizing the quantities of reagents and antisera needed. Data on reproducibility of the assay and calculation of IgM and IgG levels are provided.For the functional assessment of the humoral immunity of the rat, ELISA is a powerful tool. In an earlier report, assays for the titration of thymus-independent IgM antibodies to E. coli LPS and the IgM and IgG response to the thymus-dependent antigen tetanus toxoid were described. More recently it was shown that the antigen ovalbumin elicits a thymus-dependent IgM, IgG and IgE response which could be readily measured with the enzyme immunoassay, as well as a delayed-type hypersensitivity reaction. As the optimum ovalbumin concentration for both types of reactions was the same, it is concluded that the ovalbumin model offers the advantage that both humoral and cellular immunity can be studied simultaneously in the same animal.

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Enzyme Immunoassay: A Review

Cited by 110 publications

References 137 publications

Luciferin derivatives in bioluminescence‐enhanced enzyme immunoassays

Luciferin derivatives in bioluminescence‐enhanced enzyme immunoassays

Development of an ELISA-based kit for the on-farm determination of progesterone in milk

Use of the Enzyme-Linked Immunosorbent Assay (ELISA) in Immunotoxicity Testing

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