Enzyme Induction, Gene Amplification and Insect Resistance to Insecticides

Terriere, L. C.

doi:10.1007/978-1-4684-4466-7_11

Cited by 31 publications

(23 citation statements)

References 57 publications

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“…It has been suggested that similar regulatory mechanisms may govern P450 constitutive overexpression and induction, and that both contribute to the development of insecticide resistance 27, 33. Although the mechanisms of regulation for constitutive overexpression and for induction are not clear in insects, it has been reported that in mammals, the constitutive androstane receptor (CAR) and the pregnane x receptor (PXR) have been identified as key transcription factors (IFs) regulating phenobarbital (PB) induced transcription of P450s 25, 63, 64.…”

Section: Discussionmentioning

confidence: 99%

Permethrin Induction of Multiple Cytochrome P450 Genes in Insecticide Resistant Mosquitoes, Culex quinquefasciatus

Gong¹,

Li²,

Zhang³

et al. 2013

Int. J. Biol. Sci.

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The expression of some insect P450 genes can be induced by both exogenous and endogenous compounds and there is evidence to suggest that multiple constitutively overexpressed P450 genes are co-responsible for the development of resistance to permethrin in resistant mosquitoes. This study characterized the permethrin induction profiles of P450 genes known to be constitutively overexpressed in resistant mosquitoes, Culex quinquefasciatus. The gene expression in 7 of the 19 P450 genes CYP325K3v1, CYP4D42v2, CYP9J45, (CYP) CPIJ000926, CYP325G4, CYP4C38, CYP4H40 in the HAmCqG8 strain, increased more than 2-fold after exposure to permethrin at an LC50 concentration (10 ppm) compared to their acetone treated counterpart; no significant differences in the expression of these P450 genes in susceptible S-Lab mosquitoes were observed after permethrin treatment. Eleven of the fourteen P450 genes overexpressed in the MAmCqG6 strain, CYP9M10, CYP6Z12, CYP9J33, CYP9J43, CYP9J34, CYP306A1, CYP6Z15, CYP9J45, CYPPAL1, CYP4C52v1, CYP9J39, were also induced more than doubled after exposure to an LC50 (0.7 ppm) dose of permethrin. No significant induction in P450 gene expression was observed in the susceptible S-Lab mosquitoes after permethrin treatment except for CYP6Z15 and CYP9J39, suggesting that permethrin induction of these two P450 genes are common to both susceptible and resistant mosquitoes while the induction of the others are specific to insecticide resistant mosquitoes. These results demonstrate that multiple P450 genes are co-up-regulated in insecticide resistant mosquitoes through both constitutive overexpression and induction mechanisms, providing additional support for their involvement in the detoxification of insecticides and the development of insecticide resistance.

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Section: Discussionmentioning

confidence: 99%

Permethrin Induction of Multiple Cytochrome P450 Genes in Insecticide Resistant Mosquitoes, Culex quinquefasciatus

Gong¹,

Li²,

Zhang³

et al. 2013

Int. J. Biol. Sci.

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“…Terriere and Yu (1974) suggested that higher induction in resistant fl ies was likely due to (P450) gene amplifi cation, but they rejected induction as a factor in the development of resistance in the fi eld. It was suggested that the same regulatory (trans-acting) gene may be involved in both induction and biochemical resistance (Terriere, 1983), and Plapp (1984) further proposed that a receptor involved in xenobiotic induction may be altered in resistant insects. However, despite growing evidence that overexpression of some P450 genes in resistant strains can involve trans-regulating factors (e.g., Sabourault et al, 2001), a link with induction has remained tenuous.…”

Section: Discussionmentioning

confidence: 99%

Xenobiotic response in Drosophila melanogaster: Sex dependence of P450 and GST gene induction

Goff

Hilliou

Siegfried

et al. 2006

Insect Biochemistry and Molecular Biology

146

109

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“…The simultaneous use of biochemical markers of resistance indicated a discrepancy in the evolution of both cytochrome P450-dependent monooxygenases (MFO) and glutathione S-transferases (GST) production; the former decreased while the latter remained stable over time under the same insecticide-free conditions. In insects, there is evidence that systems of detoxification such as MFO, GST, and carboxylesterases are inducible by the presence of various insecticides (Terriere, 1983;Feyereisen, 1999). To date, the role of induction mechanisms on enzyme expression still remains a black box in C. pomonella.…”

Section: Discussionmentioning

confidence: 99%

Constraints on adaptive mutations in the codling moth Cydia pomonella (L.): measuring fitness trade-offs and natural selection

et al. 2003

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Adaptive changes in populations encountering a new environment are often constrained by deleterious pleiotropic interactions with ancestral physiological functions. Evolutionary responses of populations can thus be limited by natural selection under fluctuating environmental conditions, if the adaptive mutations are associated with pleiotropic fitness costs. In this context, we have followed the evolution of the frequencies of insecticide-resistant mutants of Cydia pomonella when reintroduced into an untreated environment. The novel set of selective forces after removal of insecticide pressure led to the decline of the frequencies of resistant phenotypes over time, suggesting that the insecticideadapted genetic variants were selected against the absence of insecticide (with a selective coefficient estimated at 0.11). The selective coefficients were also estimated for both the major cytochrome P450-dependent monooxygenase (MFO) and the minor glutathione S-transferase (GST) systems (0.17 and negligible, respectively), which have been previously shown to be involved in resistance. The involvement of metabolic systems acting both through xenobiotic detoxification and biosynthetic pathways of endogenous compounds may be central to explaining the deleterious physiological consequences resulting from pleiotropy of adaptive changes. The estimation of the magnitude of the fitness cost associated with insecticide resistance in C. pomonella suggests that resistance management strategies exclusively based on insecticide alternations would be unlikely to delay such a selection process.

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Enzyme Induction, Gene Amplification and Insect Resistance to Insecticides

Cited by 31 publications

References 57 publications

Permethrin Induction of Multiple Cytochrome P450 Genes in Insecticide Resistant Mosquitoes, Culex quinquefasciatus

Permethrin Induction of Multiple Cytochrome P450 Genes in Insecticide Resistant Mosquitoes, Culex quinquefasciatus

Xenobiotic response in Drosophila melanogaster: Sex dependence of P450 and GST gene induction

Constraints on adaptive mutations in the codling moth Cydia pomonella (L.): measuring fitness trade-offs and natural selection

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