Enzyme-linked immunosorbent assay and immunobullous disease

Heymann, Warren R.

doi:10.1016/j.jaad.2008.09.043

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Using these tests, a plethora of clinical and experimental data have been generated, which decisively influenced our knowledge on disease pathogenesis and are firmly implemented in the diagnosis and monitoring of pemphigoid diseases [1,49]. In addition to IgG and IgE, IgA autoantibodies are present to different extent in pemphigoid diseases.…”

Section: Discussionmentioning

confidence: 99%

Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Csorba

Schmidt

Florea

et al. 2011

Orphanet J Rare Dis

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BackgroundPemphigoids are rare diseases associated with IgG, IgE and IgA autoantibodies against collagen XVII/BP180. An entity of the pemphigoid group is the lamina lucida-type of linear IgA disease (IgA pemphigoid) characterized by IgA autoantibodies against BP180. While for the detection of IgG and IgE autoantibodies specific to collagen XVII several ELISA systems have been established, no quantitative immunoassay has been yet developed for IgA autoantibodies. Therefore, the aim of the present study was to develop an ELISA to detect IgA autoantibodies against collagen XVII in the sera of patients with pemphigoids.MethodsWe expressed a soluble recombinant form of the collagen XVII ectodomain in mammalian cells. Reactivity of IgA autoantibodies from patients with IgA pemphigoid was assessed by immunofluorescence microscopy and immunoblot analysis. ELISA test conditions were determined by chessboard titration experiments. The sensitivity, specificity and the cut-off were determined by receiver-operating characteristics analysis.ResultsThe optimized assay was carried out using sera from patients with IgA pemphigoid (n = 30) and healthy donors (n = 105). By receiver operating characteristics (ROC) analysis, an area under the curve of 0.993 was calculated, indicating an excellent discriminatory capacity. Thus, a sensitivity and specificity of 83.3% and 100%, respectively, was determined for a cut-off point of 0.48. As additional control groups, sera from patients with bullous pemphigoid (n = 31) and dermatitis herpetiformis (n = 50), a disease associated with IgA autoantibodies against epidermal transglutaminase, were tested. In 26% of bullous pemphigoid patients, IgA autoantibodies recognized the ectodomain of collagen XVII. One of 50 (2%) of dermatitis herpetiformis patients sera slightly topped the cut-off value.ConclusionsWe developed the first ELISA for the specific and sensitive detection of serum IgA autoantibodies specific to collagen XVII in patients with pemphigoids. This immunoassay should prove a useful tool for clinical and translational research and should essentially improve the diagnosis and disease monitoring of patients with IgA pemphigoid. Moreover, our findings strongly suggest that IgA pemphigoid and IgG bullous pemphigoid represent two ends of the clinical spectrum of an immunological loss of tolerance against components of hemidesmosomes, which is mediated by both IgG and IgA autoantibodies.

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Section: Discussionmentioning

confidence: 99%

Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Csorba

Schmidt

Florea

et al. 2011

Orphanet J Rare Dis

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“…Enzyme‐linked immunosorbent assay (ELISA) provides a more sensitive tool for autoantibody detection against the PV target antigens than immunofluorescence . This also appears to be the case for detecting IgA antibodies in serum to Dsg .…”

Section: Discussionmentioning

confidence: 99%

Serum and salivary IgG and IgA antibodies to desmoglein 3 in mucosal pemphigus vulgaris

et al. 2016

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“…However, concerns on the hazards caused by radioactive wastes, the expensive nature of counting equipment and the need for special laboratory facilities created the need for a cheaper, more efficient and risk-free assay technique that uses enzymes to label antibodies and proteins for analysis. Today, it has found useful applications in diagnosis of diseases, monitoring therapy in patients and offers insights into pathogenesis of diseases (6).…”

Section: Introductionmentioning

confidence: 99%

Gene expression and its application in biotechnology

Barde¹,

Hussaini²,

Uzoma³

et al. 2021

GSC Adv. Res. Rev.

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Advances in biotechnology has been the subject of praise for a decade now, this is due to techniques such as gene expression which has contributed immensely in the success of genetic engineering, medical advancement, vaccine production and agriculture. Gene expression has become a very important tool for the overall improvement of quality of life. This paper tries to look into the development of gene expression in the last forty years and to highlight how technological advancements in the study of gene expression brought about improvements as a focal point. Technological advancements associated with northern blotting, western blotting, and enzyme-linked immunosobent assays, Polymerase Chain Reaction (PCR) technology with emphasis on quantitative PCR, differential protein display technique and DNA sequencing and hybridization arrays technology with emphasis on macroarrays and microarrays in facilitating gene expression will be discussed.

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Enzyme-linked immunosorbent assay and immunobullous disease

Cited by 5 publications

References 9 publications

Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Serum and salivary IgG and IgA antibodies to desmoglein 3 in mucosal pemphigus vulgaris

Gene expression and its application in biotechnology

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