Enzyme-linked immunosorbent assay (ELISA): method capabilities and its use in diagnostics

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a method for detecting antigens and antibodies in biological fluids. The method is characterized by high accuracy and sensitivity, and allows determining not only the qualitative, but also the quantitative composition of antigens and antibodies in the body. In medicine, enzyme immunoassay is used to detect diseases that are provoked by microorganisms of variious classes, from viruses to parasites, as well as to diagnose autoimmune diseases.