Enzyme linked immunosorbent assay (ELISA) for paraquat

Aspectos gerais e diagnóstico clinicolaboratorial da intoxicação por paraquat

“…Vários métodos para determinação do paraquat têm sido descritos na literatura, incluindo métodos colorimétricos (13) , cromatográficos (1,9,19,21,29,39,40,51,72) , eletroforéticos (71,74,76) e imunológicos (7,18,25,48) .…”

“…Os métodos imunológicos podem ser utilizados para triagem através de imunoensaio enzimático (1) ; ou na quantificação do paraquat através de radioimunoensaio (RIA) em soro (25) ; plasma, urina e fluido do lavado broncoalveolar utilizando anticorpos monoclonais (7) ; por enzyme linked immunosorbent assay (ELISA) em plasma (48) e imunoensaios com fluorescência em plasma (18) . Segundo Proudfoot et al (56) , a sobrevida do intoxicado depende da concentração da substância no organismo e do tempo após a ingestão.…”

Development of an ELISA for paraquat; improvement of antibody characteristics by reversed affinity chromatography

“…Although the derivatives are similar, several methods of conjugation to protein have been utilised (carbodiimide, diazotization and mixed anhydride). Anti-PQ antibodies have been used in radioimmunoassay, 19 fluoroimmunoassay 20 and ELISA 13,21,22 for measuring PQ in a variety of matrices. The assay performance described here compares well with the most sensitive of the assays, the ELISA developed by Van Emon et al, 13 detecting minimum levels of PQ of 10 pg per well.…”

Determination of paraquat residues in soil by an enzyme linked immunosorbent assay

“…Keyhole limpet haemocyanin (KLH, Calbiochem, La Jolla, CA) and bovine serum albumin (BSA, BDH Chemicals) were coupled to the adduct (2) , derived from 6-bromohexanoic acid (BSA) and monoquat (3), via a carbodiimide reaction, as reported previously by Niewola et aZ. 7 The resulting conjugates contained 662 mol of paraquat per mole of KLH and 15 mol of paraquat per mole of BSA. The amount of paraquat bound to the protein was determined by a spectrophotometric dithionite assay for paraquat and the protein concentration was established by a standard Lowry test.…”