Enzyme Linked Immunosorbent Assay for Fumonisin B1 Detection in Local Corn Seeds from Baghdad-Iraq

Mohammed, Sinai W.; Nayyef, Hanan J.; Sameer, Fadhaa O.; Hanoon, Ahmed Y.

doi:10.24996/ijs.2021.62.12.3

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…In the study performed [ 58 ], the production of fumonisin B 1 is 6.22 ± 0.89 ppb. Similarly, Mohamed et al, published that FB 1 was found in all silos samples and corn markets with a range of 13.69-175.54 ppb [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Mahmoud,

Kilany,

Hassan

2023

BMC Microbiol

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Background Toxigenic fungi (Aspergillus and Fusarium) and their metabolites represent the major cause of corn and corn-based products contamination and consequently lead to severe economic and health issues. Aim Our current study aimed to investigate the efficacy of using L. macroides Bac6 as a biological control agent against the toxigenic fungi; A. flavus f10 and F. proliferatum f30 and their mycotoxins. Results The results illustrated that A. flavus f10 produced the aflatoxins AFB1 and AFG2 with concentrations of 21.239 and 13.593 ppb, respectively. While F. proliferatum f30 produced fumonisin B1 (9600 ppb). Furthermore, L. macroides showed a high potential for inhibition of toxigenic fungal growth using a dual culture method. F. proliferatum f30 and A. flavus f10 were found to be inhibited by a percentage of 80 and 62.5%, respectively. The results were confirmed using the scanning electron microscope. The antagonistic bacteria, L. macroides, showed chitinase productivity and activity of 26.45 U/L and 0.12 U/mL/min, respectively, which illustrates its potential application as a biocontrol agent. The GC-MS analysis revealed an abundance of Pyrrolo[1,2-a] pyrazine-1,4-dione, Hexahydro in the bacterial supernatant that exhibited antifungal characteristics. L. macroides had a significant reduction of AFB1 and AFG2 produced by A. flavus f10, recording 99.25% and 99% inhibition, respectively. It also showed strong inhibition of fumonisin B1 (90% inhibition) produced by F. proliferatum f30. Conclusion: Thus, the current study is a prospective study evaluating for the first time the potential impact of L. macroides Bac6 against the toxigenic fungi and their toxins.

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Section: Discussionmentioning

confidence: 99%

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Mahmoud,

Kilany,

Hassan

2023

BMC Microbiol

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Studying the Efficiency of Fermented Local Corn on Some Physiological and Immunological Characteristics of Broiler

Hamza

Hammod

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The study was carried out in the poultry farm of the Department of Animal Production, Faculty of Agriculture, University of Kufa to examine the efficiency of fermented local corn on some physiological and immunological characteristics of broiler. 300 of one day old broiler (Ross 308), unsex with initial weight of 39g were used. Chicks were raised for 35 days in closed house divided into pens with 3m2 for each and distributed randomly on 5 treatments with three replicates for each treatment (60 chicks for each treatment and 20 for each replicate). Treatments were prepared as follows: T1 (imported corn), T2 (unfermented local corn), T3 (replacing fermented local corn instead of imported corn in a ratio of 50%), T4 (replacing fermented local corn instead of imported corn in a ratio of 75%) and T5 (replacing fermented local corn instead of imported corn in a ratio of 100%). Results showed that fermenting local corn by Saccharomyces cerevisiae (1g/kg corn for 24h) was improved the nutritional value of corn particularly crude protein and fat as well as decreasing B1 aflatoxin to very low level. There were non significant differences between Haematological blood characteristics (hemoglobin, packed cell volume (PCV), red blood cell and white blood cell), biochemical characteristics (total protein, albumin, globulin, cholesterol and glucose) and immunological characteristics (antibody titers against Newcastle disease, antibody titers against avian infectious bronchitis, the relative weight of fabricius gland and the index of fabricius gland) in all Experimental treatments.

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Determination of some mycotoxins from poultry feed in Baghdad city

Ibrahim,

Taher,

Abbas

et al. 2024

APJMBB

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The aims of this study were to determine some mycotoxins and to isolate the fungi from chicken feed samples from broiler and layer farms in Baghdad city. The isolation rate (percentage) of fungi from poultry feed samples was Aspergillus niger 17.7%, Aspergillus flavus 14.7%, Aspergillus fumigatus 12.4%, Aspergillus ochraceus 10.7%, Penicillium spp. 9.3%, Fusarium spp. 9.3%, Rhizopus spp. 8.4%, Mucar spp. 8.4%, Absidia spp. 7.6% and Chrysonilia sitophila 1.5%, while the most common yeast genera were Candida spp. 44.8%, Candida albicans 20.7%, Cryptococcus neoformans 17.3%, Geotricum candidum 12% and Trichosorom spp. 5.2%. The mycotoxins ochratoxin A (OTA), deoxynivalenol (DON), fumonisin B1 (FB1) and zearalenone (ZON) were detected, and the percentages of these mycotoxins were 43.1%, 27.2%, 21.1% and 8.6%, respectively. This study indicates that the presence of mold that produce mycotoxins in poultry feed increases the risk of mycotoxins in feed, meat, eggs, etc. of animals and therefore humans, and we need to raise awareness of the Ministry of Agriculture and Health to prevent these measures to reduce the mycotoxin levels in poultry products.

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Enzyme Linked Immunosorbent Assay for Fumonisin B1 Detection in Local Corn Seeds from Baghdad-Iraq

Cited by 3 publications

References 20 publications

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Studying the Efficiency of Fermented Local Corn on Some Physiological and Immunological Characteristics of Broiler

Determination of some mycotoxins from poultry feed in Baghdad city

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