Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus

Peters, Robert L.; Collins, Michael J.; O'Beirne, A J; Howton, P A; Hourihan, Sara L.; S, Thomas

doi:10.1128/jcm.10.4.595-597.1979

Cited by 18 publications

(3 citation statements)

References 4 publications

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“…The ELISA has found wide application in the detection of antibodies and small amounts of antigen and seems to be particularly suitable for widespread epizootiological studies. The assay has been used for the detection of bovine coronavirus in feces (Ellens et al, 1978a) and for the serology of several coronaviruses, including feline infectious peritonitis virus (Osterhaus et al, 1979), murine hepatitis virus (Peters et al, 1979, Kraaijeveld et al, 1980a and human coronavirus strain 229E {Kraaijeveld et al, 1980a, b).…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Callebaut

Debouck

Pensaert

1982

Veterinary Microbiology

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An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected in PED.

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Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Callebaut

Debouck

Pensaert

1982

Veterinary Microbiology

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“…Fixative and coupling agents for improving antigen retention on support media, such as formaldehyde (12,25), glutaraldehyde (1,55,64), acetone (57), and poly-L-lysine (14,37) have also been used. Most ELISA protocols for detection of coronavirus antibodies thus far published (including that of Osterhaus et al [36] for coronavirus antibodies in cats) have used carbonatebicarbonate or borate buffer diluents at pH 9.2 to 9.8 for antigen adsorption, without additional chemical modification (9,15,24,30,36,45,46,58). Both infected culture fluids and gradientpurified coronavirus have been used as antigens in these assays.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats

Barlough¹,

Jacobson²,

Downing³

et al. 1983

J Clin Microbiol

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A computer-assisted, kinetics-based enzyme-linked immunosorbent assay was adapted for the detection of coronavirus antibodies in feline serum. An alkaline antigen diluent (carbonate-bicarbonate buffer, pH 9.6) used in initial experiments produced diffuse, nonspecific color reactions in both viral and control antigen cuvettes which were correlated, paradoxically, with coronavirus antibody levels in test sera. These interfering reactions were minimized by use of lower-pH antigen diluents such as water and phosphate-buffered saline. Background kinetics-based enzyme-linked immunosorbent assay reactivity directed against a noncoronaviral component of antigen tissue culture fluids could then detected in numerous sera, particularly in samples with lower titers. Much of this reactivity was shown to be associated with bovine gamma globulins in cell culture fluid. It was not serum lot or species specific, since a variety of bovine serum lots as well as individual lots of serum from other mammalian and avian species reacted. Reactivity was markedly reduced when cells for antigen preparation were grown in gamma globulin-free bovine serum. Generation of corrected slope values from the kinetics-based enzyme-linked immunosorbent assay made it possible to correct for residual background reactivity in individual test sera and thus eliminate a potentially major source of false-positive reactions. Collectively, these studies indicated that the control of nonspecific reactivity in feline coronavirus serology is absolutely essential to obtain useful estimates of specific antibody responses.

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“…Despite reports of more sensitive serological tests, it was several years before these methods were generally used in rodent virus serology laboratories. Both the conventional enzyme-linked immunosorbent assay (ELISA) and indirect imunofluorescence (IFA) test were shown to be significantly more sensitive than the CF test for detection of antibody to MHV (Peters et al, 1979;Boorman et al, 1982;Smith, 1983). A commercially available ELISA kit was somewhat less sensitive than IFA for detection of MHV antibody in sera of experimentally infected mice (Smith, 1983).…”

Section: Introductionmentioning

confidence: 99%

Two enzyme immunoassays for the detection of antibody to rodent coronaviruses

Smith

Winograd

1986

Journal of Virological Methods

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Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.

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Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus

Cited by 18 publications

References 4 publications

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea

Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats

Two enzyme immunoassays for the detection of antibody to rodent coronaviruses

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