Enzyme-Linked Immunosorbent Assay for Hepatitis B Surface Antigen

Wolters, G. H. J.; Kuijpers, L; Kacaki, J; Schuurs, A.H.W.M.

doi:10.1093/infdis/136.supplement_2.s311

Cited by 56 publications

(21 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TNF‐α was evaluated in serum according to the method described by Wolters et al. using a TNF‐α ELISA kit provided by Alpco® Diagnostics Distributer (Mumbai, India), and the concentrations were measured from the standard curve. The TBA reactive substances levels were measured as MDA according to the method of Mihara and Uchiyama, and the concentrations were measured from the standard curve constructed using serial dilution of 1,1,3,3‐tetramethoxypropane.…”

Section: Methodsmentioning

confidence: 99%

Alpha lipoic acid prevents doxorubicin-induced nephrotoxicity by mitigation of oxidative stress, inflammation, and apoptosis in rats

El-Sayed

Mansour

El-Sawy

2017

J Biochem Mol Toxicol

View full text Add to dashboard Cite

This study aimed to evaluate the protective effects of alpha lipoic acid (ALA) against doxorubicin (DOX)-induced nephrotoxicity in rats. A single dose of DOX (7.5 mg/kg i.v.) induced nephrotoxicity evidenced by significant elevations in kidney weight, kidney/body weight ratio, serum urea, creatinine, tumor necrosis factor alpha, and renal contents of malondialdehyde, nitric oxide, cyclooxygenase-2, and caspase-3. Also, it causes significant reduction in final body weight, serum albumin, renal contents of reduced glutathione and superoxide dismutase activity. Histopathological changes in the kidney tissue confirmed the nephrotoxic effect. In contrast, pretreatment with ALA (50 mg/kg, orally) for 14 days before DOX and for 7 days after DOX administration mitigated renal toxicity evidenced by greater improvement in the examined oxidative stress, inflammation, and apoptosis parameters. In conclusion, ALA had promising protective effects against DOX-induced nephrotoxicity that might be attributed to its antioxidant, anti-inflammatory, and antiapoptoic activities.

show abstract

Section: Methodsmentioning

confidence: 99%

Alpha lipoic acid prevents doxorubicin-induced nephrotoxicity by mitigation of oxidative stress, inflammation, and apoptosis in rats

El-Sayed

Mansour

El-Sawy

2017

J Biochem Mol Toxicol

View full text Add to dashboard Cite

show abstract

“…ELISA was used to measure TNF‐α level (rat TNF‐α kit, BioSource International Inc., Camarillo, CA). The method utilizes the immunoassay technique 25. Plasma samples were pipetted into rat TNF‐α‐specific antibody coated wells, and second (biotinylated) antibody was added.…”

Section: Methodsmentioning

confidence: 99%

The protective effect of aminoguanidine on doxorubicin‐induced nephropathy in rats

Abo-Salem

2012

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Reactive oxygen species and cytokines have been implicated in the nephrotoxicity induced by doxorubicin. The goal of the present study was to determine protective effect of aminoguanidine on doxorubicin-induced nephrotoxicity in rats. Different groups of male Wistar rats received doxorubicin (67.75 mg/kg/i.p./2 days), aminoguanidine alone and aminoguanidine (200 and 400 mg/kg/i.p./30 days) prior to doxorubicin, respectively. Doxorubicin significantly increased serum creatinine (505%), blood urea nitrogen (333%), nitric oxide (406%), and plasma tumor necrosis factor-alpha (706%) as well as urinary albumin (452%) and N-acetyl-β-D-glucosaminidase (415%) compared to control. Moreover, renal glutathione (334%), superoxide dismutase (283%), and catalase (513%) were significantly reduced accompanied with elevation in renal malondialdehyde compared to control. Pretreatment with aminoguanidine mitigated such changes in all mentioned parameters. Histopathological changes showed that doxorubicin-caused significant structural damages to kidneys that were reduced with aminoguanidine. Results indicate that reactive oxygen species and cytokines are involved in doxorubicin-induced nephrotoxicity, which can be reduced by aminoguanidine.

show abstract

“…Urea and creatinine concentrations were measured spectrophotometrically according to the methods of Tabacco et al [30] and Fabiny and Ertingshausen [31], respectively. TNF-α was determined according to the method of Wolters et al [32].…”

Section: Measurement Of Creatine Kinasementioning

confidence: 99%

Thymoquinone supplementation attenuates cyclophosphamide‐induced cardiotoxicity in rats

Nagi

Al‐Shabanah

Hafez

et al. 2010

J Biochem & Molecular Tox

View full text Add to dashboard Cite

This study examined the possible protective effects of thymoquinone (TQ), the main constituent of the volatile oil of black seed (Nigella sativa), against cyclophosphamide (CP)-induced cardiotoxicity. Adult male Wistar albino rats were divided into four treatment groups. Rats in the first group were served as control. Rats in the second group received TQ (50 mg/L in drinking water) for 12 days. Animals in the third group were injected with a single dose of CP (200 mg/kg, IP) at day 5. Rats in the fourth group received TQ (50 mg/L in drinking water) for 5 days before a single dose of CP (200 mg/kg, IP) and continued thereafter throughout the experiment. On day 13, animals were sacrificed; serum and hearts were isolated and analyzed. Cyclophosphamide resulted in a significant increase in serum creatine kinase, lactate dehydrogenase, cholesterol, triglycerides, creatinine, urea, and tumor necrosis factor-α. In heart tissues, CP resulted in a significant increase in thiobarbituric acid reactive substances and total nitrate/nitrite and a significant decrease in reduced glutathione, glutathione peroxidase, catalase, and adenosine triphosphate levels. Interestingly, TQ supplementation resulted in a complete reversal of all the biochemical changes induced by CP to their control values. Data from this study suggest that TQ supplementation attenuates CP-induced cardiotoxicity by a mechanism related, at least in part, to its ability to decrease oxidative and nitrosative stress and to preserve the activity of antioxidant enzymes as well as its ability to improve the mitochondrial function and energy production. .

show abstract

Enzyme-Linked Immunosorbent Assay for Hepatitis B Surface Antigen

Cited by 56 publications

References 6 publications

Alpha lipoic acid prevents doxorubicin-induced nephrotoxicity by mitigation of oxidative stress, inflammation, and apoptosis in rats

Alpha lipoic acid prevents doxorubicin-induced nephrotoxicity by mitigation of oxidative stress, inflammation, and apoptosis in rats

The protective effect of aminoguanidine on doxorubicin‐induced nephropathy in rats

Thymoquinone supplementation attenuates cyclophosphamide‐induced cardiotoxicity in rats

Contact Info

Product

Resources

About