Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid

Yoshida, Minoru; Hamada, Takahiro; Amagai, Masayuki; Hashimoto, Koji; Uehara, Rie; Yamaguchi, Kentaro; Imamura, Koji; Okamoto, Eiji; Yasumoto, Shinichiro; Hashimoto, Takashi

doi:10.1016/j.jdermsci.2005.11.002

Cited by 138 publications

(131 citation statements)

References 43 publications

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“…In our study, performed in patients with typical BP, we found similar percentages of positivity for IIF and ELISA tests at diagnosis compared to previously published studies, where BP180 ELISA sensitivity ranges from 79 to 96% and the one of BP230 ELISA from 58 to 61% [6,9,10,11,12]. Consequently, in our study, we confirm that BP230 ELISA seems of less value for the diagnosis of typical BP than IIF and BP180 ELISA.…”

Section: Commentsupporting

confidence: 90%

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The Value of BP230 Enzyme-Linked Immunosorbent Assay in the Diagnosis and Immunological Follow-Up of Bullous Pemphigoid

Peufeilhoux¹,

Ingen‐Housz‐Oro²,

Hüe³

et al. 2012

Dermatology

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Background: The value of 230-kDa bullous pemphigoid antibody (BP230) enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bullous pemphigoid (BP) was investigated, but in the immunological follow-up of the disease remains unknown. Objective: Evaluation of BP230 ELISA for diagnosis, follow-up and prediction of relapse in BP. Methods: Monocenter retrospective and prospective study. Patients with typical BP. Detection of autoantibodies by indirect immunofluorescence (IIF), BP180 and BP230 ELISA tests at diagnosis, during the treatment (disease control or failure) and at treatment stop (relapse or not 3 months after). Results: 74 patients were included. At diagnosis, BP230 ELISA sensitivity was lower than IIF and BP180 ELISA. Combining both ELISA added a weak gain of sensitivity. Both tests paralleled the clinical evolution, especially in case of disease control. At the end of the treatment, BP230 ELISA was not different in patients with or without relapse. Conclusion: In routine practice, BP230 ELISA does not seem to be a useful additional test in typical BP.

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Section: Commentsupporting

confidence: 90%

“…Several studies focused on BP230 ELISA for BP diagnosis, showing a sensitivity lower than that of BP180 ELISA (50–60%) but a specificity >90% [9,10,11,12]. However, the value of BP230 ELISA for the immunological follow-up of the disease and in the prediction of relapse is still unknown.…”

Section: Introductionmentioning

confidence: 99%

The Value of BP230 Enzyme-Linked Immunosorbent Assay in the Diagnosis and Immunological Follow-Up of Bullous Pemphigoid

Peufeilhoux¹,

Ingen‐Housz‐Oro²,

Hüe³

et al. 2012

Dermatology

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“…The use of ELISA are necessary to determine the protein productivity of the clones [7][8][9], and His tags to aid the purification of recombinant proteins have been widely used [10][11][12]. For obtaining cloned cell lines, we have applied the target gene and a selection marker gene in which the expression vectors.…”

Section: Resultsmentioning

confidence: 99%

Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone

Hu¹,

Liu²,

Han³

et al. 2017

Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

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Abstract. Recombinant follicle stimulating hormone(rFSH), which play an important role in assisted reproductive technologies. However, the availability of a suitable system for the screening high production cell lines from transfected mammal cells for the production of rFSH is limited. The purpose of this study was to establish a method for screening cloned cell lines with high productivity from transfected HEK293 cells. Here, we adopt the antibiotics in combination with ELISA as a hyphenated technique to obtain production cell lines, and the results indicated that this a simple and efficient method in the cloned cell screening application.

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“…The BIOCHIP ® mosaic assay specifically detects IgG auto-antibodies bound to the respective auto-antigens. Circulating anti-BP230 IgG auto-antibodies were determined in parallel by 2 different ELISA systems, using an E. coli-expressed fragment of the C-terminal globular domain of BP230 (Euroimmun; BP230-C; amino acids 2326-2649) and both the recombinant N-and C-terminal fragments (MBL, Nagoya, Japan; BP230-N-C; amino acids 1-979 and 1869-2649) (12,13). All assays were performed according to the manufacturers' instructions.…”

Section: Methodsmentioning

confidence: 99%

Value of BIOCHIP Technology in the Serological Diagnosis of Pemphigoid Gestationis

Sadik¹,

Pas²,

Bohlmann³

et al. 2017

Acta Derm Venerol

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Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid

Cited by 138 publications

References 43 publications

The Value of BP230 Enzyme-Linked Immunosorbent Assay in the Diagnosis and Immunological Follow-Up of Bullous Pemphigoid

The Value of BP230 Enzyme-Linked Immunosorbent Assay in the Diagnosis and Immunological Follow-Up of Bullous Pemphigoid

Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone

Value of BIOCHIP Technology in the Serological Diagnosis of Pemphigoid Gestationis

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