Enzyme-modified nanoporous gold-based electrochemical biosensors

Qiu, Hua‐Jun; Xue, Luyan; Ji, Guanglei; Zhou, Guiping; Huang, Xirong; Qu, Yinbo; Gao, Pin

doi:10.1016/j.bios.2009.03.011

Cited by 152 publications

(80 citation statements)

References 21 publications

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“…oxidase [134,140,141], alcohol dehydrogenase [141], xylanase [142], acetylcholine esterase [66], cytochrome c [135], ligin peroxidase [143], lipase [139], catalase [139], horseradish peroxidase [139], bovine serum albumin [90], and immunoglobulin G [90]. Strategies for immobilization have included physical, electrostatic, and covalent attachment.…”

Section: Dna Sensorsmentioning

confidence: 99%

“…More recently, there have also been several studies on the actual development of nanoporous-gold-based biosensors [140,141]. For example, in one of the first reports on this subject, Qiu and coworkers constructed an electrochemical biosensor for glucose or ethanol using either glucose oxidase (GOD) or alcohol dehydrogenase (ADH), respectively, immobilized on nanoporous gold (NPG) (pore size ∼40 nm) formed by chemically dealloying gold leaf in nitric acid and captured on a glassy carbon electrode (GCE) [141]. Nafion was coated on the enzyme-modified electrodes to hold the enzymes in place.…”

Section: Dna Sensorsmentioning

confidence: 99%

“…The authors showed that the Nafion/ADH-NPG/GCE electrode had a fast response time with millimolar concentrations of ethanol, good sensitivity, and a detection limit of 120 M [141]. For the detection of glucose, the authors showed that the Nafion/GOD-nanoporous gold/glassy carbon electrode had good sensitivity at mM concentrations and a detection limit of 196 M [141]. In both cases, the response was significantly better at the nanoporous gold electrode compared to that prepared from a gold sheet electrode.…”

Section: Dna Sensorsmentioning

confidence: 99%

“…The amperometric response of nanoporous gold electrodes to NADH and hydrogen peroxide compared to planar gold has been examined [141]. For the oxidation of NADH, the authors report a linear range of 0.02 to 1 mM and a detection limit of 9.5 M and state that it is better than that observed at the planar electrode.…”

Section: Small Molecule Sensingmentioning

confidence: 99%

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Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Collinson

2013

ISRN Analytical Chemistry

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Nanoporous gold prepared by dealloying Au:Ag alloys has recently become an attractive material in the field of analytical chemistry. This conductive material has an open, 3D porous framework consisting of nanosized pores and ligaments with surface areas that are 10s to 100s of times larger than planar gold of an equivalent geometric area. The high surface area coupled with an open pore network makes nanoporous gold an ideal support for the development of chemical sensors. Important attributes include conductivity, high surface area, ease of preparation and modification, tunable pore size, and a bicontinuous open pore network. In this paper, the fabrication, characterization, and applications of nanoporous gold in chemical sensing are reviewed specifically as they relate to the development of immunosensors, enzyme-based biosensors, DNA sensors, Raman sensors, and small molecule sensors.

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Section: Dna Sensorsmentioning

confidence: 99%

Section: Dna Sensorsmentioning

confidence: 99%

Section: Dna Sensorsmentioning

confidence: 99%

Section: Small Molecule Sensingmentioning

confidence: 99%

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Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Collinson

2013

ISRN Analytical Chemistry

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“…On the other hand, some dependent reagents in the preparation of nanomaterial produced a negative impact on the fabricated biosensor. 26,27 Moreover, particle aggregation often existed in gold nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode

Zhong¹

2012

IJN

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Abstract:In this paper, a sensitive chronocoulometric deoxyribonucleic acid (DNA) biosensor based on a nanostructure gold electrode was fabricated for detection of the femtomolar level survivin gene which was correlated with osteosarcoma by using hexaamine-ruthenium III complexes, [Ru(NH 3 , as the electrochemical indicator. The effect of different frequencies on the real surface area of the nanostructure gold electrode obtained by repetitive square-wave oxidation reduction cycle was investigated. At the optimal frequency of 8000 Hz, the real surface of the developed nanostructure gold electrode was about 42.5 times compared with that of the bare planar gold electrode. The capture probe DNA was immobilized on the nanostructure gold electrode and hybridized with target DNA. Electrochemical signals of hexaamine-ruthenium III bound to the anionic phosphate of DNA strands via electrostatic interactions were measured by chronocoulometry before and after hybridization. The increase of the charges of hexaamineruthenium III was observed upon hybridization of the probe with target DNA. Results indicate that this DNA biosensor could detect the femtomole (fM) concentration of the DNA target quantitatively in the range of 50 fM to 250 fM; the detection limit of this DNA biosensor was 5.6 fM (signal to noise = 3). This new biosensor exhibits excellent sensitivity and selectivity and has been used for an assay of polymerase chain reaction (PCR) with a satisfactory result.

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