Enzyme Multiplied Immunoassay Technique: A Review

Abstract

A brief review of the various immunoassays is presented before the basic mechanism of the enzyme multiplied immunoassay technique (EMIT) is described. This is followed by a presentation of the specific advantages and disadvantages of this method, as well as its correlation with other methods as applied to qualitative and quantitative determinations of each type of drug for which EMIT technology is available.

Improved Sensitivity for Methotrexate Analysis Using Enzyme Multiplied Immunoassay Technique on the Siemens Viva-E Instrument

“…3 The use of EMIT assays for therapeutic drug monitoring has been particularly useful. 3 The use of EMIT assays for therapeutic drug monitoring has been particularly useful.…”

Processing Validation Metrics of Syva Enzyme-Multiplied Immunoassay Technique (EMIT) Methotrexate Assay for Beckman Coulter System

“…In addition, the detection of low MTX concentrations allows for the assessment of toxic concentrations that may exist 72 hours after high-dose delivery of the drug [ 6 ]. As enzyme-multiplied immunoassay technique (EMIT) assays have been of importance for therapeutic drug monitoring, it has been long used for the determination of both endogenous and exogenous analytes in such clinical settings [ 7 ]. Syva EMIT MTX (Gurgaon, India: Siemens Healthcare Diagnostics Ltd.) assay uses a homogeneous enzyme immunoassay method.…”

Clinical Monitoring of Therapeutic Drugs

“…Undoubtedly the biggest single fillip to the growth of clinical interest in the measurement of therapeutic drugs in blood has been the development and application of immunoassay techniques (Mould et al, 1977;Curtis and Patel, 1978;Marks, 1979). This is at least partly due to the extreme simplicity with which assays can be carried out in the average hospital laboratory using commercially available reagent kits, their speed, precision and accuracy, and potential universal applicability.…”