Enzyme producing insect gut microbes: an unexplored biotechnological aspect

Banerjee, Sandipan; Maiti, Tushar Kanti; Roy, Raj Narayan

doi:10.1080/07388551.2021.1942777

Cited by 56 publications

(26 citation statements)

References 152 publications

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“…However, the study concluded that the isolated strain works poorer outside the living host than inside the mealworm's gut. The mealworm gut act as an efficient bioreactor for PS degradation (Banerjee et al, 2022). Similarly, the yellow mealworms were also being tested for their PS degradation ability and have shown degrade half of the supplemented PS within 12-15 h inside the gut.…”

Section: Relevance Of Microbes In Plastic Degradationmentioning

confidence: 99%

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

Shilpa

Basak

Meena

2022

Front. Environ. Sci. Eng.

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Section: Relevance Of Microbes In Plastic Degradationmentioning

confidence: 99%

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

Shilpa

Basak

Meena

2022

Front. Environ. Sci. Eng.

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“…Cooperation and competition within microbial communities based on nutrient exchange are ubiquitous. Insect fitness and growth under low nutrient levels can be boosted by microorganisms through complex interactions with enzymes and their environment. ,, The supplementation of nutrition containing diets, especially wheat bran, a primary feedstock for commercial mealworm production, does enhance plastic biodegradation in the mealworms. ,,− ,, However, the role of key gut environmental structures or diets with different nutrition sources in determining community dynamics and stability during plastic degradation remains poorly studied. Especially, the impact of nutrition levels in the co-diets on plastic degradation, structures of gut microbiome, and metabolic functions remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiome Associating with Carbon and Nitrogen Metabolism during Biodegradation of Polyethene in Tenebrio larvae with Crop Residues as Co-Diets

Ding

Yang

Ding

et al. 2023

Environ. Sci. Technol.

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Tenebrio molitor and Tenebrio obscurus (Coleoptera: Tenebrionidae) larvae are two commercial insects that eat plant and crop residues as diets and also biodegrade synthetic plastics polyethylene (PE). We examined biodegradation of low-density PE (LDPE) foam (M n = 28.9 kDa and M w = 342.0 kDa) with and without respective co-diets, i.e., wheat brain (WB) or corn flour (CF), corn straw (CS), and rice straw (RS) at 4:1 (w/w), and their gut microbiome and genetic metabolic functional groups at 27.0 ± 0.5 °C after 28 days of incubation. The presence of co-diets enhanced LDPE consumption in both larvae and broad-depolymerized the ingested LDPE. The diet type shaped gut microbial diversity, potential pathways, and metabolic functions. The sequence of effectiveness of co-diets was WB or CF > CS > RS for larval development and LDPE degradation. Co-occurrence networks indicated that the larvae co-fed with LDPE displayed more complex correlations of gut microbiome than the larvae fed with single diets. The primary diet of WB or CF and crop residues CS and RS provided energy and nitrogen source to significantly enhance LDPE biodegradation with synergistic activities of the gut microbiota. For the larvae fed LDPE and LDPE plus co-diets, nitrogen fixation function was stimulated compared to normal diets and associated with LDPE biodegradation.

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“…The majority of protease producing insect gut microbes are bacteria, including Firmicutes , Proteobacteria, and Actinobacteria. only one fungal member Ascomycota has been reported to produce proteases ( Idowu et al, 2009 ; Banerjee et al, 2022 ). Issatchenkia spp.…”

Section: Discussionmentioning

confidence: 99%

“…To achieve efficient digestion, the proteases secreted by the midgut require a suitable pH. Insect gut pH plays an important role in the digestion of ingested food ( Banerjee et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Enhanced protein degradation by black soldier fly larvae (Hermetia illucens L.) and its gut microbes

Zhang

Zhu

et al. 2023

Front. Microbiol.

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Black soldier fly larvae (BSFL) can convert a variety of organic wastes into biomass, and its gut microbiota are involved in this process. However, the role of gut microbes in the nutrient metabolism of BSFL is unclear. In this study, germ-free BSFL (GF) and gnotobiotic BSFL (GB) were evaluated in a high-protein artificial diet model. We used 16S rDNA sequencing, ITS1 sequencing, and network analysis to study gut microbiota in BSFL that degrade proteins. The protein reduction rate of the GB BSFL group was significantly higher (increased by 73.44%) than that of the GF BSFL group. The activity of gut proteinases, such as trypsin and peptidase, in the GB group was significantly higher than the GF group. The abundances of different gut microbes, including Pseudomonas spp., Orbus spp. and Campylobacter spp., were strongly correlated with amino acid metabolic pathways. Dysgonomonas spp. were strongly correlated with protein digestion and absorption. Issatchenkia spp. had a strong correlation with pepsin activity. Campylobacter spp., Pediococcus spp. and Lactobacillus spp. were strongly correlated with trypsin activity. Lactobacillus spp. and Bacillus spp. were strongly correlated with peptidase activity. Gut microbes such as Issatchenkia spp. may promote the gut proteolytic enzyme activity of BSFL and improve the degradation rate of proteins. BSFL protein digestion and absorption involves gut microbiota that have a variety of functions. In BSFL the core gut microbiota help complete protein degradation. These results demonstrate that core gut microbes in BSFL are important in protein degradation.

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Enzyme producing insect gut microbes: an unexplored biotechnological aspect

Cited by 56 publications

References 152 publications

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

Gut Microbiome Associating with Carbon and Nitrogen Metabolism during Biodegradation of Polyethene in Tenebrio larvae with Crop Residues as Co-Diets

Enhanced protein degradation by black soldier fly larvae (Hermetia illucens L.) and its gut microbes

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