Enzyme production from different fruit and vegetable waste using lactic acid fermentation

Garg, Neelima; Singh, Balvindra; Vaish, Supriya; Kumar, Sanjay

doi:10.5958/2582-2683.2021.00014.9

Cited by 7 publications

(3 citation statements)

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“…In their study, Ozcelik and Yaruz-Duzgum (2016) pointed out that fruit and vegetable waste serve as substantial reservoirs of carbohydrates, proteins, vitamins, and minerals. Researchers (Neelima et al, 2021) demonstrate that these resources find application in generating value-added items like phytochemicals, biofuels and enzymes. By employing low-cost or surplus substrates such as waste from fruit processing, it becomes plausible to lower enzyme production expenses.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Cellulase Production via Response Surface Methodology From Cladosporium Sp.: Fruit Waste Addition Impact

Poludasu

2024

J microb biotech food sci

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Accumulating organic waste stands as a significant challenge in developing nations. Researchers have extensively studied and utilized sugarcane bagasse fiber residues to produce various compounds. Cladosporium sp. NCIM 901 employs peracetic acid (PAA) treated bagasse in solid substrate fermentation (SSF) to generate cellulase enzyme. We utilized a Box-Behnken Design (BBD) to investigate how the fermentation medium components - watermelon juice, lemon peel powder, KH2PO4, (NH4)2SO4, and MgSO4 - affect the synthesis of Filter Paperase (FPase), Carboxymethyl cellulase (CMCase), and β-glucosidase enzymes. Under the enhanced conditions, the trial exhibited β-glucosidase, CMCase, and FPase activities of 18.6, 19.4, and 17.6 U/gds, respectively. The model calculated R2 values ranging between 91.2–91.4%, suggesting their suitability and potentially usable for estimating the level of culture medium variables needed to achieve optimal enzyme generation by the fungal strain Cladosporium sp. Observations confirm that supplementing lemon peel powder and watermelon juice with MgSO4 enhances enzyme production. The findings of model validation revealed high consistency between the observed experimental and anticipated outcomes. In the simultaneous saccharification and fermentation (SsF) process, the processed bagasse yielded more ethanol (26 g/L) compared to the untreated substrate. Based on the result, it can be conclude that, fruit processing wastes are useful for increasing cellulase enzyme production by fungi under solid state fermentation.

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Section: Introductionmentioning

confidence: 99%

Enhancing Cellulase Production via Response Surface Methodology From Cladosporium Sp.: Fruit Waste Addition Impact

Poludasu

2024

J microb biotech food sci

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“…The valorization of organic fruit processing wastes and byproducts into biofertilizers reduces the price of inorganic fertilizer, increases crop productivity, and lowers costs related to waste management practices 43,44 . Fruit wastes are also a good alternative material for enzyme production, acting as catalysts, 45 and help to save energy and chemicals in comparison with the conventional process of producing various products in large‐scale processing industries. This is a win‐win approach that enables the companies to comply with stringent legislation that strongly encourages processing industries to implement a circular economy (i.e., reduce and manage their waste) 20,39 …”

Section: Introductionmentioning

confidence: 99%

Valorization of tropical fruit‐processing wastes and byproducts for biofuel production

Gebre,

Gebremariam,

Keneni

et al. 2023

Biofuels Bioprod Bioref

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Global energy demand is expanding due to an increasing population and the use of energy‐intensive technologies. Fossil‐based energy and transportation sectors have been blamed for greenhouse gas emissions and price fluctuations. Biofuel production has been suggested as a possible solution to this problem, despite the cost of production and difficulties with the availability of feedstocks, and the food versus energy debate, which is still a challenging issue. For many decades, researchers have been investigating how to valorize low‐cost and non‐edible biomass resources, including fruit‐processing waste and its byproducts, to produce various biofuels using innovative, cost‐effective, and green technologies to overcome these challenges. However, exhaustive studies and comprehensive and structured knowledge related to the biofuel production potential of fruit waste and byproducts are lacking because most of the research that has been done so far was specific to a single fruit, location, and method. As a result, this review paper emphasizes the evaluation of the physicochemical compositions of the wastes and byproducts derived from tropical fruit production and processing to determine their suitability in the production of different biofuels via various valorization techniques. The results of the review showed that fruit waste is a potential alternative raw material for producing different biofuels, like biodiesel, biogas, biohydrogen, and fuel pellets.

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“…Recently, Garg et al (2021) reported Lactobacillus plantarum for enzyme production using fruit and vegetable waste as substrate. Citrus is one of important fruit crops of India with a production of 13.4 million metric tonnes during 2018-19.…”

Section: Introductionmentioning

confidence: 99%

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Singh¹,

Garg²,

Mathur³

et al. 2022

JEB

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Aim: To determine feasibility of using mosambi peel as substrate for multi enzyme production through microbial intervention. Methodology: Thirty-three bacterial isolates were isolated from biodegradable organic substrates and investigated in-vitro their biodegradable activities viz. pectinolytic, cellulolytic and amylolytic. The best performing bacterial isolate, exhibiting aforesaid activities were selected and identified using 16S rRNA technique. The factors, that influenced the fermentation, viz. temperature, pH and incubation period of bacterial culture were optimized for maximum production of multi enzymes viz. pectinase, cellulase and amylase using mosambi peel as substrate under solid-state fermentation conditions. Molecular weights of different enzymes present in multi-enzyme preparation were determined by SDS PAGE. The juice extraction efficiency of crude multi enzyme preparation was compared with that of pure commercial enzyme. Results: Out of thirty-three bacterial isolates, after primary and secondary screening, the best performing bacterial isolate was identified as Bacillus subtilis strain NG 105 (Genbank accession number MN493055). Higher enzyme activities were observed at pH 7.0, incubation temperature of 35°C and incubation period of 10 days for pectinase, cellulase and amylase using mosambi peel as substrate. The purified enzymes characterized by SDS-PAGE, revealed to have molecular mass of 65 kDa for pectinase, 50 kDa for cellulase and 55 kDa for amylase. Bagasse fibre was found to be the most suited matrix for immobilization. The juice extraction efficiency of partially purified crude multienzyme preparation was 88% of commercial pectinase. Interpretation: Mosambi peel is a suitable substrate for multienzyme production using Bacillus subtilis under solid-state fermentation condition.

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Enzyme production from different fruit and vegetable waste using lactic acid fermentation

Cited by 7 publications

References 0 publications

Enhancing Cellulase Production via Response Surface Methodology From Cladosporium Sp.: Fruit Waste Addition Impact

Enhancing Cellulase Production via Response Surface Methodology From Cladosporium Sp.: Fruit Waste Addition Impact

Valorization of tropical fruit‐processing wastes and byproducts for biofuel production

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

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