Enzymes Application in Diagnostic Prospects

Raja, M.; Raja, A.; Imran, Muhammad; Sántha, A; Devasena, K.

doi:10.3923/biotech.2011.51.59

Cited by 32 publications

(14 citation statements)

References 11 publications

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“…A focus on these neglected enzyme groups (isomerases, transferases, and synthetases) should not only extend the spectrum using AuNPsbased enzyme assays, but also inspire the development of AuNPsbased assays for new targets. [136][137][138] Despite the benefits presented by these nano diagnostic systems, there is still a long way to go before the extensive application of colorimetric AuNPs can offer effective diagnostic tests that can be translated into the laboratory or testing near patient setting.…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticle-based colorimetric biosensors

et al. 2018

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Gold nanoparticles (AuNPs) provide excellent platforms for the development of colorimetric biosensors as they can be easily functionalised, displaying different colours depending on their size, shape and state of aggregation. In the last decade, a variety of biosensors have been developed to exploit the extent of colour changes as nano-particles (NPs) either aggregate or disperse, in the presence of analytes. Of critical importance to the design of these methods is that the behaviour of the systems has to be reproducible and predictable. Much has been accomplished in understanding the interactions between a variety of substrates and AuNPs, and how these interactions can be harnessed as colorimetric reporters in biosensors. However, despite these developments, only a few biosensors have been used in practice for the detection of analytes in biological samples. The transition from proof of concept to market biosensors requires extensive long-term reliability and shelf life testing, and modification of protocols and design features to make them safe and easy to use by the population at large. Developments in the next decade will see the adoption of user friendly biosensors for point-of-care and medical diagnosis as innovations are brought to improve the analytical performances and usability of the current designs. This review discusses the mechanisms, strategies, recent advances and perspectives for the use of AuNPs as colorimetric biosensors.

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Section: Discussionmentioning

confidence: 99%

Gold nanoparticle-based colorimetric biosensors

et al. 2018

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“…The isolated Lactobacillus spp. can tolerate a wide range of pH (1)(2)(3)(4)(5)(6)(7)(8)(9) and grow well at acidic pH (1)(2)(3)(4)(5). NaCl is an inhibitory substance which may inhibit growth of certain types of bacteria and probiotic organisms have to withstand high salt concentration in human gut [18].…”

Section: Isolate-y 1 Isolate -Y 2 Isolate-y 3 Isolate-ymentioning

confidence: 99%

“…Probiotics such as Lactobacillus spp. are reported to have inhibitory activity against common human pathogens [5][6][7]. They are able to produce antimicrobial substances such as bacteriocins which have great potential to be used in therapeutics and as food bio-preservatives [8].…”

Section: Introductionmentioning

confidence: 99%

Screening of Lactobacillus spp. from Buffalo Yoghurt for Probiotic and Antibacterial Activity

Chowdhury¹,

Hossain²,

Mostazir³

et al. 2012

J Bacteriol Parasitol

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Since the beneficial effects of viable probiotic bacteria as dietary supplements have gained huge research interest, Lactobacillus spp. with probiotic characteristics are widely used to prepare fermented dairy products such as yoghurts, milk-shakes etc. In this study, eight (08) homemade yoghurt samples were collected from different regions in the country for isolation of probiotic Lactobacillus spp. Among the samples, four (04) isolates were identified as Lactobacillus plantarum based on their growth and biochemical characteristics. The Isolates were resistant to NaCl (1-9%) and bilesalt (0.05-0.3%) and showed good growth in the acidic condition, while maximum growth was observed at pH around 6.0. The isolates were examined for their antibacterial activity against nine (09) different test pathogens and found all pathogens are inhibited their growth to some extent but maximum zone of inhibition was observed against Bacillus cereus (53.20 mm) and minimum was against Staphylococcus aureus (19 mm) after 72 hour incubation. The results of the present study indicate that, homemade yoghurts in Bangladesh are potential source of probiotic Lactobacillus spp. Further extensive research on isolation and characterization of probiotic organisms from local fermented foods and their growth optimization might be required for development of probiotic enriched food supplements in our country.

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“…Enzymes play a critical role in the metabolic activities of all living organisms and are widely applied in biotechnology. Abnormality of the enzyme metabolism systems leads to a number of metabolic diseases [90]. Diseases associated with components of the enzyme metabo-lism or with the enzyme activities are broadly applied in clinical examinations as special markers as some examples displayed on Table 3.…”

Section: Enzyme Based Biosensormentioning

confidence: 99%