Eph Receptor Effector Ephexin Mediates Olfactory Dendrite Targeting in <i>Drosophila</i>

Sardana, Juhi; Organisti, Christina; Kadow, Ilona C. Grunwald

doi:10.1002/dneu.22624

Cited by 5 publications

(7 citation statements)

References 42 publications

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“…Olfactory sensory neurons located on the antennae or palps connect to projection neurons (PNs) in the antennal lobe. These connections are highly stereotyped across individuals [9][10][11][12][13] . Expectedly, the responses of PNs to odors are also stereotyped 14,15 .…”

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confidence: 99%

Multiple network properties overcome random connectivity to enable stereotypic sensory responses

et al. 2020

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Connections between neuronal populations may be genetically hardwired or random. In the insect olfactory system, projection neurons of the antennal lobe connect randomly to Kenyon cells of the mushroom body. Consequently, while the odor responses of the projection neurons are stereotyped across individuals, the responses of the Kenyon cells are variable. Surprisingly, downstream of Kenyon cells, mushroom body output neurons show stereotypy in their responses. We found that the stereotypy is enabled by the convergence of inputs from many Kenyon cells onto an output neuron, and does not require learning. The stereotypy emerges in the total response of the Kenyon cell population using multiple odor-specific features of the projection neuron responses, benefits from the nonlinearity in the transfer function, depends on the convergence:randomness ratio, and is constrained by sparseness. Together, our results reveal the fundamental mechanisms and constraints with which convergence enables stereotypy in sensory responses despite random connectivity.

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confidence: 99%

Multiple network properties overcome random connectivity to enable stereotypic sensory responses

et al. 2020

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“…As described above, Vav physically binds to the Eph receptor in Drosophila S2 cells. This receptor is known to play a critical role in a wide range of neuronal processes, including local dendrite branch dynamics, axon navigation, and synapse formation in Drosophila (Anzo et al, 2017; Boyle et al, 2006; Frank et al, 2009; Sardana et al, 2018). We thus sought to investigate whether Eph controls dendritic outgrowth in the aCC motoneurons.…”

Section: Resultsmentioning

confidence: 99%

The Vap33/Eph/Vav/Cdc42 complex confers temporal specification to the outgrowth of primary dendrites in Drosophila neurons

Kamiyama¹,

Nishida²,

Kamiyama³

et al. 2022

Preprint

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The formation of primary dendrites (dendritogenesis) significantly affects the overall orientation and coverage of dendritic arborization, limiting the number and types of inputs a neuron can receive. Previously we reported how a Drosophila motoneuron spatially controls the positioning of dendritogenesis through the Dscam1/Dock/Pak1 pathway; however, how the neuron defines the timing of this process remains elusive. Here we show that the Eph receptor tyrosine kinase provides a temporal cue. We find that, at the onset of dendritogenesis, the Eph receptor recruits the Rho Family GEF Vav to the intracellular domain of Eph, which transiently activates the Cdc42 family of small GTPase. We also show that vap33 (vesicle-associated membrane protein-associated protein) mutants exhibit defects in Cdc42 activation and dendritic outgrowth, indicating Vap33 may play an upstream role in Eph signaling. Together, our result and previous studies argue that the formation of primary dendrites requires the proximity of active Cdc42 and membrane-anchored Pak1 driven by collaborative action between two distinct signaling complexes, Vap33/Eph/Vav and Dscam1/Dock. Signal integration from multiple input pathways would represent a general mechanism for the spatiotemporal precision of dendrite branch formation.

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“…(ARHGEF5/TIM1), Ephexin4 (ARH GEF16), and Ephexin5 (ARHGEF15/Vsm-RhoGEF) 9,21,[29][30][31][32] . It has been reported that Ephexin1 and Ephexin5 are phosphorylated by Src or Eph receptors but not ARH-GEF16 21,29,33 .…”

Section: Discussionmentioning

confidence: 99%

FYN is required for ARHGEF16 to promote proliferation and migration in colon cancer cells

Chen

et al. 2020

Cell Death Dis

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ARHGEF16 is a recently identified Rho-family guanine nucleotide exchange factor (GEF) that has been implicated in the activation of Rho-family GTPases such as Rho G, Rac, and Cdc42. However, its functions in colon cancer cell proliferation and migration are not well understood. In this study, we showed that ARHGEF16 was highly expressed in clinical specimens of colon cancer. In colon cancer cells, ARHGEF16-stimulated proliferation and migration in vitro and in vivo. Furthermore, we identified a nonreceptor tyrosine kinase, FYN, as a novel partner of ARHGEF16. Knocking down FYN expression decreased ARHGEF16 protein level in colon cancer cells. We further demonstrated that ARHGEF16-induced colon cancer cell proliferation and migration were dependent on FYN since knockdown FYN abolished the ARHGEF16-induced proliferation and migration of colon cancer cells. The FYN-ARHGEF16 axis mediates colon cancer progression and is a potential therapeutic target for colon cancer treatment.

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Eph Receptor Effector Ephexin Mediates Olfactory Dendrite Targeting in Drosophila

Cited by 5 publications

References 42 publications

Multiple network properties overcome random connectivity to enable stereotypic sensory responses

Multiple network properties overcome random connectivity to enable stereotypic sensory responses

The Vap33/Eph/Vav/Cdc42 complex confers temporal specification to the outgrowth of primary dendrites in Drosophila neurons

FYN is required for ARHGEF16 to promote proliferation and migration in colon cancer cells

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