Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis

Abstract

To evaluate the usefulness of phenotypic and genotypic analyses for the epidemiologic typing of methicillinresistant Staphylococcus aureus (MRSA), we characterized 64 epidemic MRSA isolates and 10 sporadic methicillin-susceptible S. aureus isolates from a university hospital and 18 MRSA isolates from hospitals in different geographical areas. Chromosomal DNA macrorestriction analysis with Sstll was resolved by pulsed-field gel electrophoresis and compared with antibiotype analysis, phage type analysis, and standard genomic DNA restriction analysis with BgM. Indices of the discriminatory ability of these methods were 0.982,

Eradication of a resistant Pseudomonas aeruginosa strain after a cluster of infections in a hematology/oncology unit

“…Another group, consisting of the patient isolates and the environmental isolates from ward 5, possessed a dissimilar PFGE profile which differed from that of the former group by three bands. This is consistent with two closely related but separate strains being present, as a single point mutation could produce a three-band difference [4]. The remaining piperacillin-tazobactam resistant isolates had unique PFGE profiles which were distinct from either of the other two strains.…”

Clonal dissemination of epidemic methicillin-resistant Staphylococcus aureus in Belgium and neighboring countries

“…The similarity of macrorestriction patterns was determined both by visual comparison and by computer matching. Patterns differing by more than three fragments were considered to be distinct clones, and those differing by 1-3 fragments subclonal variants or subclones [6]. Polaroid negatives were scanned using the ScanJet II P system (Hewlett Packard, Brussels, Belgium) into TIFF files which were imported in GelCompar version 4.0 (Applied Maths, Kortrijk, Belgium).…”

“…A dendrogram of similarity was built using the unweighted pair-group method using arithmetic averages (UPGMA). Clonal group level was set at − 80% similarity according to previously published data [6].…”

“…Epidemiologic typing of MRSA can be achieved by a number of molecular methods [5][6][7][8][9][10][11][12]. Pulsed-field gel electrophoresis (PFGE) of genomic macrorestriction fragments has been recommended as a standard [13].…”

Epidemiologic Typing of Methicillin‐Resistant Staphylococcus aureus in Neonate Intensive Care Units Using Pulsed‐Field Gel Electrophoresis

“…In order to break the cycle of dissemination of colonization, type-investigation of the prevailing MRSA is necessary. Although traditional methods of strain typing, such as bacteriophage typing and serotyping, may be inadequate for the purpose of epidemiological analysis of nosocomial pathogens, recently newer molecular methods, particularly the analysis of chromosomal DNA restriction patterns by pulsed-field gel electrophoresis (PFGE), have come to be regarded as useful methods for investigating the source, transmission, and spread of nosocomial MRSA infection (4,7,8,12,14,21,24). Further, phenotypic analysis, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), has been used successfully to identify different strains of Staphylococcus aureus (1,6,20,23).…”