Epidemiological features of the bovine spongiform encephalopathy epidemic in Japan

Sugiura, Katsuaki; Onodera, Takashi; Bradley, Ray

doi:10.20506/rst.28.3.1938

Cited by 6 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H-type BSE has been described in cattle from France [11], Germany [1], Japan [12], the Netherlands [13], Poland [14], Switzerland [15], the United Kingdom [16], Canada [17], and the United States [18]. The molecular phenotype of the H-type BSE cases is characterized by 1) a higher molecular mass of the unglycosylated PrP Sc isoform, 2) a strong labeling of all 3 PrP Sc polypeptides (unglycosylated, monoglycosylated and diglycosylated isoforms) with the PrP-specific monoclonal antibodies 6H4 and P4, and 3) a second unglycosylated band at approximately 14 kDa when developed with antibodies that bind in the C-terminal region (amino acids 154–236) such as SAF 84 [19].…”

Section: Introductionmentioning

confidence: 99%

Clinical and Pathologic Features of H-Type Bovine Spongiform Encephalopathy Associated with E211K Prion Protein Polymorphism

et al. 2012

View full text Add to dashboard Cite

The majority of bovine spongiform encephalopathy (BSE) cases have been ascribed to the classical form of the disease. H-type and L-type BSE cases have atypical molecular profiles compared to classical BSE and are thought to arise spontaneously. However, one case of H-type BSE was associated with a heritable E211K mutation in the prion protein gene. The purpose of this study was to describe transmission of this unique isolate of H-type BSE when inoculated into a calf of the same genotype by the intracranial route. Electroretinograms were used to demonstrate preclinical deficits in retinal function, and optical coherence tomography was used to demonstrate an antemortem decrease in retinal thickness. The calf rapidly progressed to clinical disease (9.4 months) and was necropsied. Widespread distribution of abnormal prion protein was demonstrated within neural tissues by western blot and immunohistochemistry. While this isolate is categorized as BSE-H due to a higher molecular mass of the unglycosylated PrPSc isoform, a strong labeling of all 3 PrPSc bands with monoclonal antibodies 6H4 and P4, and a second unglycosylated band at approximately 14 kDa when developed with antibodies that bind in the C-terminal region, it is unique from other described cases of BSE-H because of an additional band 23 kDa demonstrated on western blots of the cerebellum. This work demonstrates that this isolate is transmissible, has a BSE-H phenotype when transmitted to cattle with the K211 polymorphism, and has molecular features that distinguish it from other cases of BSE-H described in the literature.

show abstract

Section: Introductionmentioning

confidence: 99%

Clinical and Pathologic Features of H-Type Bovine Spongiform Encephalopathy Associated with E211K Prion Protein Polymorphism

et al. 2012

View full text Add to dashboard Cite

show abstract

“…[8][9][10][35][36][37] In harmonization with reports from EU and Canadian laboratories as described above, we carried out an evaluation study of NippiBL Ò , together with TeSeE Ò and FRELISA Ò , to assess their competence to detect atypical BSE prions.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of rapid post-mortem test kits for bovine spongiform encephalopathy (BSE) screening in Japan: Their analytical sensitivity to atypical BSE prions

Hagiwara

Iwamaru

Tabeta

et al. 2017

Prion

View full text Add to dashboard Cite

ABSTRACT. A classical type of bovine spongiform encephalopathy (C-BSE), recognized in 1987, had a large impact on public health due to its zoonotic link to variant Creutzfeldt-Jakob disease by the human consumption of dietary products contaminated with the C-BSE prion. Thus, a number of countries implemented BSE surveillance using rapid post-mortem test kits that were approved for detection of the C-BSE prion in the cattle brain. However, as atypical BSE (L-and H-BSE) cases emerged in subsequent years, the efficacy of the kits for the detection of atypical BSE prions became a matter of concern. In response to this, laboratories in the European Union and Canada evaluated the kits used in their countries. Here, we carried out an evaluation study of NippiBL Ò , a kit currently used for BSE screening in Japan. By applying the kit to cattle brains of field cases of C-BSE and L-BSE, and an experimental case of H-BSE, we showed its comparable sensitivities to C, L-, and H-BSE prions, and satisfactory performance required by the European Food Safety Authority. In addition to NippiBL Ò , two kits (TeSeE Ò and FRELISA Ò ) formerly used in Japan were effective for detection of the L-BSE prion, although the two kits were unable to be tested for the H-BSE prion due to the discontinuation of domestic sales during this study. These results indicate that BSE screening Correspondence to: Ken'ichi Hagiwara;

show abstract

“…The second type of atypical BSE is the H-type, characterized by Western blot analysis to have a higher molecular mass of the unglycosylated isoform. To date, the H-type has been described in cattle from France, 11 Germany, 16 Japan, 101 the Netherlands, 55 Poland, 55 Switzerland, 103 Until these recent atypical BSE reports, BSE has been shown to be very consistent and uniform in appearance, even after transmission to other species. There are several hypotheses proposed to explain atypical BSE cases.…”

Section: Atypical Bovine Spongiform Encephalopathy Cases: H-type and mentioning

confidence: 99%

“…The second type of atypical BSE is the H-type, character-ized by Western blot analysis to have a higher molecular mass of the unglycosylated isoform. To date, the H-type has been described in cattle from France, 11 Germany, 16 Japan, 101 the Netherlands, 55 Poland, 55 Switzerland, 103 the United Kingdom 99 and the United States. 86 The unusual molecular phenotype of the H-type BSE cases was characterized by 1) a higher molecular mass of the unglycosylated PrP d isoform, 2) a strong labeling of all 3 PrP d polypeptides (unglycosylated, monoglycosylated and diglycosylated isoforms) with the PrP-specific monoclonal antibodies 6H4 (amino acid epitope consisting of DYEDRYYRE) and P4 (amino acid epitope consisting of GGGWGQGGTHGQWNK), and 3) a glycoform profile with a less prominent diglycosylated PrP d isoform (French and U.S. cases).…”

Section: Atypical Bovine Spongiform Encephalopathy Cases: H-type and L-type Bsementioning

confidence: 99%

Experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle

et al. 2011

View full text Add to dashboard Cite

Abstract. Prion diseases or transmissible spongiform encephalopathies (TSEs) of animals include scrapie of sheep and goats; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of deer, elk and moose; and bovine spongiform encephalopathy (BSE) of cattle. The emergence of BSE and its spread to human beings in the form of variant Creutzfeldt-Jakob disease (vCJD) resulted in interest in susceptibility of cattle to CWD, TME and scrapie. Experimental cross-species transmission of TSE agents provides valuable information for potential host ranges of known TSEs. Some interspecies transmission studies have been conducted by inoculating disease-causing prions intracerebrally (IC) rather than orally; the latter is generally effective in intraspecies transmission studies and is considered a natural route by which animals acquire TSEs. The "species barrier" concept for TSEs resulted from unsuccessful interspecies oral transmission attempts. Oral inoculation of prions mimics the natural disease pathogenesis route whereas IC inoculation is rather artificial; however, it is very efficient since it requires smaller dosage of inoculum, and typically results in higher attack rates and reduces incubation time compared to oral transmission. A species resistant to a TSE by IC inoculation would have negligible potential for successful oral transmission. To date, results indicate that cattle are susceptible to IC inoculation of scrapie, TME, and CWD but it is only when inoculated with TME do they develop spongiform lesions or clinical disease similar to BSE. Importantly, cattle are resistant to oral transmission of scrapie or CWD; susceptibility of cattle to oral transmission of TME is not yet determined.

show abstract

Epidemiological features of the bovine spongiform encephalopathy epidemic in Japan

Cited by 6 publications

References 17 publications

Clinical and Pathologic Features of H-Type Bovine Spongiform Encephalopathy Associated with E211K Prion Protein Polymorphism

Clinical and Pathologic Features of H-Type Bovine Spongiform Encephalopathy Associated with E211K Prion Protein Polymorphism

Evaluation of rapid post-mortem test kits for bovine spongiform encephalopathy (BSE) screening in Japan: Their analytical sensitivity to atypical BSE prions

Experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle

Contact Info

Product

Resources

About