Epidemiological study, immunohistochemistry and in situ hybridization studies of nasopharyngeal carcinomas: A Tunisian report

Hila, Lamia; Farzaneh, Farah; Ayari, Hager; Ferjaoui, Mehdi; Dehria, W.; Jilani, Sarra Ben

doi:10.1016/j.patbio.2008.07.029

Cited by 8 publications

(8 citation statements)

References 9 publications

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“…Consistent with previous reports [7], [29], [30], the LMP1 protein levels in C666-1 and the established EBV-infected nasopharyngeal epithelial cell lines were either low or undetectable by Western blotting analysis (Fig. 1B and 1C).…”

Section: Resultssupporting

confidence: 92%

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Id1 Interacts and Stabilizes the Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) in Nasopharyngeal Epithelial Cells

et al. 2011

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The EBV-encoded latent membrane protein 1 (LMP1) functions as a constitutive active form of tumor necrosis factor receptor (TNFR) and activates multiple downstream signaling pathways similar to CD40 signaling in a ligand-independent manner. LMP1 expression in EBV-infected cells has been postulated to play an important role in pathogenesis of nasopharyngeal carcinoma. However, variable levels of LMP1 expression were detected in nasopharyngeal carcinoma. At present, the regulation of LMP1 levels in nasopharyngeal carcinoma is poorly understood. Here we show that LMP1 mRNAs are transcribed in an EBV-positive nasopharyngeal carcinoma (NPC) cell line (C666-1) and other EBV-negative nasopharyngeal carcinoma cells stably re-infected with EBV. The protein levels of LMP1 could readily be detected after incubation with proteasome inhibitor, MG132 suggesting that LMP1 protein is rapidly degraded via proteasome-mediated proteolysis. Interestingly, we observed that Id1 overexpression could stabilize LMP1 protein in EBV-infected cells. In contrary, Id1 knockdown significantly reduced LMP1 levels in cells. Co-immunoprecipitation studies revealed that Id1 interacts with LMP1 by binding to the CTAR1 domain of LMP1. N-terminal region of Id1 is required for the interaction with LMP1. Furthermore, binding of Id1 to LMP1 suppressed polyubiquitination of LMP1 and may be involved in stabilization of LMP1 in EBV-infected nasopharyngeal epithelial cells.

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Section: Resultssupporting

confidence: 92%

“…Previous studies have reported low levels or absence of protein expression in NPC cells [7], [29], [30]. In this study, we showed that LMP1 transcription could be readily detected by RT-PCR in all EBV-infected epithelial cells but the protein levels were either low or absence in Western blotting analysis (Fig.…”

Section: Discussionsupporting

confidence: 60%

Id1 Interacts and Stabilizes the Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) in Nasopharyngeal Epithelial Cells

et al. 2011

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“…Ertan et al [23] found EBER expression in 100% cases of UCNT, Hila et al [24] in 96.6% cases, Nakao et al [17] in 88% samples, etc. The literature regarding the prognostic value of EBER expression in UCNT of non-endemic region is partly limited due to small sample size [17].…”

Section: Discussionmentioning

confidence: 98%

“…The age-incidence curve available for 14 age groups (15)(16)(17)(18)(19)(20)(21)(22)(23)(24), …, 80-84) showed normal distribution with one peak (ages 50-54 years). Survival rates were significantly decreased in the age group older than 50 years (P = 0.011).…”

Section: Impact Of Different Clinical Parameters On Survivalmentioning

confidence: 99%

Prognostic significance of clinical parameters and Epstein-Barr virus infection in non-endemic undifferentiated carcinoma of nasopharyngeal type: a Serbian report

Terzić

Boricic

Pendjer³

et al. 2010

Med Oncol

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Undifferentiated carcinoma of nasopharyngeal type (UCNT) is very rare tumour in Serbia, like in most of the countries of Europe, with incidence less than 0.5 per 100,000 people per year. The aim of this study was to assess the presence of Epstein-Barr virus (EBV) in the UCNT of a non-endemic population in Serbia and identify the main clinical parameters that interfere with patients' survival rate. This study included 102 patients with UCNT who were diagnosed between 1996 and 2003. Biopsies were analysed for EBV-encoded RNA (EBER) by in situ hybridization of tumour tissue microarray. Of 102 patients, 76 were men and 26 were women with ages ranging between 18 and 82 years (median 52.5, mean 53.0±14.1). Survival rates were 80, 39 and 31% for one, three and five years, respectively. Ninety-three of 102 cases were EBER positive (92%). Factors with unfavourable prognostic values were age over 50 years at the time of diagnosis, advanced clinical stage, therapy other than chemoradiotherapy and EBER negative status. In regard to the clinical data, EBER expression in UCNT was shown to be a strong independent predictor of overall and progression-free survival. To our knowledge, the current report constitutes the largest European non-endemic series of UCNT samples from a single institution with correlation between survival and clinical parameters/EBER status.

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“…An epidemiological study has revealed that numerous factors may affect cell gene expression, which results in the occurrence of NPC (20). Among all of these factors, the environment, genes and Epstein-Barr virus infection status are the most notable (21-24).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA ZNF674‑1 acts as a cancer suppressor in nasopharyngeal carcinoma

et al. 2018

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Abstract. Nasopharyngeal carcinoma (NPC) is the most frequently occurring carcinoma of the head and neck. The complexity of NPC makes it difficult for it to be diagnosed and treated at an early stage. Certain long non-coding RNAs (lncRNAs) are closely associated with the carcinogenesis of NPC. In the present study, the expression of lncRNA ZNF674-1 in NPC tissues and an NPC cell line was analyzed and was revealed to be downregulated compared with normal tissues and cells. When the expression of lncRNA ZNF674-1 was reduced in NPC cells, the proliferation, migration and invasion of these cells was promoted, whereas the apoptosis of these cells was decreased. On the contrary, when overexpressed, the expression of lncRNA ZNF674-1 inhibited the proliferation, invasion and migration of cells, but promoted cell apoptosis. The results of the present study reveal that the lncRNA ZNF67-1 may restrain the carcinogenesis of NPC, and may also serve as a potential biomarker for the early diagnosis and treatment of NPC.

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Epidemiological study, immunohistochemistry and in situ hybridization studies of nasopharyngeal carcinomas: A Tunisian report

Cited by 8 publications

References 9 publications

Id1 Interacts and Stabilizes the Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) in Nasopharyngeal Epithelial Cells

Id1 Interacts and Stabilizes the Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) in Nasopharyngeal Epithelial Cells

Prognostic significance of clinical parameters and Epstein-Barr virus infection in non-endemic undifferentiated carcinoma of nasopharyngeal type: a Serbian report

Long non‑coding RNA ZNF674‑1 acts as a cancer suppressor in nasopharyngeal carcinoma

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