Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved

Bedford, A; Chen, Tao; Huynh, Evanna; Zhu, Cui; Medeiros, Samantha; Wey, D.; Lange, Cornelis de; Li, Julang

doi:10.1016/j.jbiotec.2015.01.007

Cited by 36 publications

(40 citation statements)

References 51 publications

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“…Bovine colostrum can restore intestinal function after initial formula-induced inflammation in preterm pigs [29]. EGF is one of the most abundant growth factors found in the milk and plays important roles in neonatal intestinal development to protect neonates against pathogens infection [30–32]. Plasma anabolic hormones, such as insulin and IGF-1, are highly associated with the local bovine mammary metabolism; highest levels of IGF-1 in milk were found 2 weeks antepartum followed by a rapid decrease during the first milking postpartum [33].…”

Section: Discussionmentioning

confidence: 99%

Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum

Tan

Wei

Yuan

et al. 2017

Journal of Immunology Research

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Colostrum is the main external resource providing piglets with nutrients and maternal immune molecules. Astragalus polysaccharides (APS) have been used as immunopotentiators in vitro and several animal models. This study aimed to determine the effects of APS on immune factors in sow colostrum and milk. The sow diet was supplemented with APS one week before the expected delivery date. Colostrum and milk were collected and designated as 0 h- (onset of parturition), 12 h-, and 24 h-colostrum and 36 h-milk postpartum. Samples were measured using porcine immunoglobulin (Ig) G, IgM, classical swine fever virus antibody (CSFV Ab), epidermal growth factor (EGF), and insulin-like growth factor- (IGF-) 1 ELISA Quantitation Kits. Dietary supplementation of APS significantly enhanced the presence of IgG, IgM, EGF, and IGF-1 in 0 h-colostrum (P < 0.001). The blocking rates of CSFV Ab were increased in samples from APS-supplemented sow when compared to those from the matched samples without APS treatment. The results indicate that supplement of APS could improve the immune components in sow colostrum and/or milk; and status of some specific vaccination could be determined through using colostrum or early milk in sow.

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Section: Discussionmentioning

confidence: 99%

Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum

Tan

Wei

Yuan

et al. 2017

Journal of Immunology Research

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“…In an attempt to unravel potential mechanisms of action, Bedford et al (2015) used 64 piglets weaned at 3 wk of age and fed them ad libitum one of two treatments for 3 wk: an EGF-containing supernatant from EGF-LL culture (SuperEGF) or a blank M17GE media (Control). The SuperEGF pigs had an increased average daily gain during week 3 after weaning (433 vs. 389 g; P < 0.05) and a better overall gain:feed ratio (0.76 vs. 0.68 kg:kg, P < 0.05).…”

Section: Growth Factors In Colostrum and Milkmentioning

confidence: 99%

Invited review: Aspects of gastrointestinal tract growth and maturation in the pre- and postweaning period of pigs

Pluske

2016

Journal of Animal Science

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ABSTRACT:The growth and development of the gastrointestinal tract (GIT) of the young pig, both before and after parturition, is critical for the animal's future growth and development, efficiency of feed conversion to body depots, and, ultimately, its survival. The perinatal development of the GIT encompasses a prenatal phase, a neonatal phase, and the postweaning phase, which is associated with the adaptation of the GIT to utilize solid feed after the piglet is weaned. The consumption of colostrum, initially, and then milk after birth provides nutrients and compounds for piglets that are critically important as stimuli and substrates to the GIT that in turn evoke a suite of anatomical, immunological, biochemical, physiological, and regulatory processes that advance the overall maturity of the GIT. However, at weaning the combined influence of the various stressors imposed causes a hiatus in the growth and development of the GIT, such that the newly weaned pig endures a "growth check" while exposing it to a greater disease and health risk. Low and variable feed intake is a major outcome of the weaning process, but nevertheless, there are numerous nutritional/management interventions producers can implement in an attempt to overcome this major issue. This review summarizes some major aspects of, and influences on, GIT growth, development, and maturation in the pre-and postweaning period of pigs, demonstrating that postnatal influences occur in utero and that evolution of the GIT continues to occur after weaning.

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“…In addition, Menard et al [25] have worked with human fetal intestine culture and they have found the EGF regulates the genes which are related with inflammation process. Bedford et al [26] have pointed out EGF therapy has the ability to increase the expression of interleukin 13 as an antiinflammatory cytokine. Therapeutic effects in experimental colitis models and the positive effects of necrotizing enterocolitis treatment have led to the use of EGF in the treatment of IBD.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the role of EGF +61A/G and EGFR R497K polymorphism in patients with inflammatory bowel disease: A case-control study

Kahraman

İplik

Çalhan

et al. 2018

Archives of Clinical and Experimental Medicine

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Aim: Epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) play an important role in the regulation of cell growth, survival, migration, apoptosis, proliferation, and differentiation. We aimed to investigate the presence of EGF (+61A/G) and EGFR R497Kpolymorphisms in patients with inflammatory bowel disease (IBD) and their associations with clinical features of the patients. Methods: This case-control study included 91 IBD patients (45 Crohn's disease (CD) patients and 46 ulcerative colitis (UC) patients) and 129 healthy controls (HC).EGF and EGFR were genotyped by polymerase chain reaction and restriction fragment length polymorphism techniques to elucidate their association with clinical outcomes. The disease activity for UC and CD were assessed by Truelove-Witts index (TW) and Crohn's disease activity index (CDAI), respectively. The Montreal classification was used for disease involvement and behavior. Results: EGFR497 AA genotype was significantly decreased in patients with UC compared with CD and HC. In addition, the patients with UC who had EGF +61 A allele had increased risk of moderate and severe disease (p=0.28; OR= 3.13; 95% CI=0.34-28.73). The patients with CD who had the EGF61 AG genotype were found to increased risk for the presence of penetrating disease (p=0.14; χ2=5.59; OR=5.00; 95% CI=1.26-19.83). EGF +61 A genotype carriers also had higher CDAI scores (p=0.19; OR=4.00; 95% CI=0. 44-36.14). In addition, A+ carriers were also found to have higher requirement for anti-TNF treatment (p=0.11; OR=5.0; 95% CI=0.56-44.4). Conclusion: In this study, EGFR 497 AA genotype was found to decrease significantly in patients with UC compared to HC and CD patients. To enlighten the mechanism, further studies with larger sample groups are needed to clarify the role of the EGF (+61A/G) and EGFR R497K genes polymorphism, and development of the etiology and pathogenesis of IBD. Keywords: EGF61, EGFR497, inflammatory bowel diseases, Crohn's disease, ulcerative colitis Öz Amaç: Epidermal büyüme faktörü (EGF) ve epidermal büyüme faktörü reseptörü (EGFR), hücre büyümesi, canlılığı, migrasyonu, apoptoz, proliferasyon ve farklılaşmasının düzenlenmesinde önemli bir rol oynamaktadır. İnflamatuar barsak hastalığı (İBH) olan hastalarda EGF (+61A/G) ve EGFR R497K polimorfizmlerinin varlığını ve hastalığın klinik özellikler ile ilişkisini araştırmayı amaçladık. Yöntemler: Bu vaka kontrol çalışmasında 91 IBD hastası (45 Crohn hastalığı (CD) hastası ve 46 ülseratif kolit (UC) hastası) ve 129 sağlıklı kontrol (HC) vardı. EGF ve EGFR, polimeraz zincir reaksiyonu ve restriksiyon fragman uzunluğu polimorfizm teknikleri ile hastalık ve sağlıklı control grubu genotiplendirildi. Genotiplerinhastalık ve klinik özellikleri ile ilişkileri incelendi. UC ve CD için hastalık aktivitesi sırasıyla Truelove-Witts indeksi (TW) ve Crohn hastalığı aktivite indeksi (CDAI) ile değerlendirildi. Montreal sınıflandırması hastalık tutulumu ve davranışı için kullanılmıştır. Bulgular: Ulseratif kolit hastalarında EGFR497 AA genotipi CD v...

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Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved

Cited by 36 publications

References 51 publications

Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum

Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum

Invited review: Aspects of gastrointestinal tract growth and maturation in the pre- and postweaning period of pigs

Assessment of the role of EGF +61A/G and EGFR R497K polymorphism in patients with inflammatory bowel disease: A case-control study

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