Epidermal growth factor in human follicular fluid stimulates mouse oocyte maturation in vitro

Das, Kamalini; Phipps, William R.; Hensleigh, Hugh C.; Tagatz, George E.

doi:10.1016/s0015-0282(16)54977-2

Cited by 54 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since the late 1980s, EGF has been reported to contribute to cumulus cell mucification and expansion and oocyte maturation (Dekel & Sherizly 1985, Downs et al 1988, Downs 1989. Later reports suggested the presence of locally produced EGF and/or other factors within the mammalian follicle (Das et al 1992, Gomez et al 1993, Fujinaga et al 1994, and that EGF receptors may potentiate gonadotropin action in granulosa cells (Hattori et al 1995). Recently, published data from Park et al (2004) and Ashkenazi et al (2005) have showed that a series of EGF-like factors (EREG, AREG and BTC) are produced by granulosa cells after the LH ovulatory stimulus.…”

Section: Discussionmentioning

confidence: 99%

Epiregulin can effectively mature isolated cumulus–oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles

Romero

Smitz²

2009

Reproduction

View full text Add to dashboard Cite

REPRODUCTION RESEARCHEpiregulin can effectively mature isolated cumulus-oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles Sergio Romero and Johan SmitzFollicle Biology Laboratory (FOBI), Vrije Universiteit Brussel (VUB), UZ Brussel, Laarbeeklaan 101, Brussels 1090, BelgiumCorrespondence should be addressed to S Romero; Email: sromerol@vub.ac.be AbstractEpiregulin mediates LH ovulatory effects in vitro. This study evaluated the use of epiregulin as an alternative to hCG/epidermal growth factor (EGF) stimulus upon cultured ovarian follicles in contrast to isolated cumulus-oocyte complexes (COCs). Pre-antral mouse ovarian follicles were cultured for 12 days and final maturation was induced by administration of 0.65 nM EGF or 100 nM epiregulin without or with 1.2 IU/ml hCG. Results showed that both EGF or epiregulin as sole stimulators are poor inducers of mucification/expansion of cumulus cells and oocyte meiotic reinitiation in follicle-enclosed COCs (25G17 and 22G16% GVBD respectively; versus 97G4 and 90G15% GVBD by control hCG/EGF and hCG/epiregulin respectively; meanGS.D). Furthermore, EGF or epiregulin did not induce follicle luteinisation: progesterone production was marginally increased and oestradiol was incompletely shut down. Supposing that the sub-normal progesterone secretion was a potential cause for incomplete meiosis in this model, effectiveness of progesterone supplementation and addition of a progesterone receptor inhibitor (RU486) were evaluated on meiotic resumption. Progesterone was not found to be a major regulator of meiosis in this mouse model. Epiregulin induced meiosis more effectively in COCs isolated from cultured preovulatory follicles in a secondary culture well. In conclusion, epiregulin has similar effects as EGF upon fully grown follicles. Used as a sole stimulator of periovulatory events in intact cultured follicles, both are poor inducers of follicle luteinisation and oocyte maturation. By contrast, epiregulin is as efficient as hCG/EGF, when used as meiotic stimulator for COCs isolated from the follicular environment (mural granulosa and theca cells; and conditioned medium).

show abstract

Section: Discussionmentioning

confidence: 99%

Epiregulin can effectively mature isolated cumulus–oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles

Romero

Smitz²

2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…Although LH plays a role as the first trigger in these events, both cumulus cells and oocytes possess few or no LH receptors, and thus are insensitive to direct LH stimulation (Peng et al, 1991). It is well accepted that epidermal growth factor (EGF) stimulates oocyte maturation and cumulus expansion in mouse, rat, hamster, porcine and human (Das et al, 1992;Dekel and Sherizly, 1985;Downs, 1989;Goud et al, 1998;Hinokio et al, 2002;Prochazka et al, 2000). Recently, Park JY et al reported that LH stimulation induces the transient sequential expression of the EGF family members like amphiregulin, epiregulin and beta-cellulin, and incubation of follicle with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation (Park et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice

et al. 2006

View full text Add to dashboard Cite

“…Specific effects of EGF on the developmental competence of mouse COCs in vitro include promotion of meiotic maturation with increased rates of polar body formation, and significantly improved cumulus expansion (Das et al 1992). Enhanced cumulus expansion due to EGF stimulation in bovine COCs is blocked by roscovitine, an inhibitor of maturation/M-phase promoting factor (MPF) kinase activity (Vigneron et al 2003) and the tyrosine kinase activity of the EGF receptor (EGFR) is required for EGF-stimulated expansion in pig COCs (Prochazka et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor activates cytosolic [Ca2+] elevations and subsequent membrane permeabilization in mouse cumulus–oocyte complexes

O’Donnell¹,

Hill²,

Gross³

2004

Reproduction

View full text Add to dashboard Cite

The role of epidermal growth factor (EGF) in the maturation of mammalian oocytes is well known but not well characterized. This membrane permeabilization leads to loss of cell contents and presumptive cumulus cell death. This catastrophic EGF-induced plasma membrane permeabilization of individual cumulus cells within a COC leads to pulsatile Ca 21 efflux as previously seen, and may lead to improved cumulus cell expansion during COC maturation.

show abstract

Epidermal growth factor in human follicular fluid stimulates mouse oocyte maturation in vitro

Cited by 54 publications

References 18 publications

Epiregulin can effectively mature isolated cumulus–oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles

Epiregulin can effectively mature isolated cumulus–oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles

The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice

Epidermal growth factor activates cytosolic [Ca2+] elevations and subsequent membrane permeabilization in mouse cumulus–oocyte complexes

Contact Info

Product

Resources

About