Epidermal growth factor receptor immunoreactivity in rat brain. Development and cellular localization

Gómez‐Pinilla, Fernando; Knauer, Daniel J.; Nieto–Sampedro, Manuel

doi:10.1016/0006-8993(88)91369-8

Cited by 170 publications

(97 citation statements)

References 17 publications

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“…The combination of serum-free medium with the anti-mitotic treatment attenuated glial proliferation. In agreement with the previous reports, a subpopulation of GABAergic cells expressed one of EGF receptor subtypes, ErbB1, and bound to fluorescent-labeled EGF (Gómez-Pinilla et al, 1988;Kornblum et al, 1995). Moreover, the GluR1-reducing activity of TGFα was apparent not only in AMPA binding activity per milligram protein but also on per culture dish basis: If the mitotic activity of TGFα contributed to the change in AMPA binding, then glial proliferation should either not influence or increase AMPA binding per dish, because astrocytes also express functional AMPA receptors (Seifert and Steinhauser, 2001;Iino et al, 2001).…”

Section: Distinction Between Mitotic Activity and Ampa Receptor-reducsupporting

confidence: 93%

“…Therefore, we mainly focused on GABAergic neurons and investigated GluR1 expression in this population. The expression of EGF receptors (ErbB1) in cultured GABAergic neurons was confirmed using the anti-ErbB1 antibody (Gómez-Pinilla et al, 1988;Kornblum et al, 1995). In neocortical cultures, a majority of GAD67-positive cells (approximately 74%) were immunoreactive to the anti-ErbB1 antibody (Fig.…”

Section: Effect Of Tgfα On Glur1 Immunoreactivitymentioning

confidence: 86%

“…A significant proportion of cultured cortical neurons including GABAergic neurons, which were prepared from rat embryos at late gestation, were positive for ErbB1 and/or ErbB4 (Yau et al, 2003;Fox and Kornblum, 2005). Even at later postnatal stages, ErbB1 immunoreactivity or mRNA is detected in basket-like cells or GABAergic neurons in the neocortex (Gómez-Pinilla et al, 1988;Kornblum et al, 1995;Fox and Kornblum, 2005). Therefore, the downregulation of AMPA-type glutamate receptor presumably represents the direct action of TGFα and any other ErbB1 ligands on developing GABAergic neurons.…”

Section: Biological Implication Of Tgfα Actionmentioning

confidence: 99%

“…Their receptor, ErbB1, is distributed in not only glial cells but also in neurons (Kornblum et al, 1995). In particular, ErbB1 is enriched in GABAergic neurons and dopaminergic neurons (Gómez-Pinilla et al, 1988;Kornblum et al, 1995Kornblum et al, , 1997. Mice lacking ErbB1 exhibit abnormal cortical development, including loss of astrocytes and impaired neuronal migration (Threadgill et al, 1995;Kornblum et al, 1998;Sibilia et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Transforming growth factor alpha attenuates the functional expression of AMPA receptors in cortical GABAergic neurons

Namba

Nagano

Iwakura

et al. 2006

Molecular and Cellular Neuroscience

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In the developing neocortex, brain-derived neurotrophic factor (BDNF) exerts a trophic activity to increase the expression and channel activity of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptor subunits. Here, we demonstrate that the epidermal growth factor (EGF) receptor (ErbB1) ligands exert the opposite biological activity in cultured neocortical neurons. Subchronic stimulation of ErbB1 with transforming growth factor α (TGFα), EGF, or heparin-binding EGF (HB-EGF) down-regulated protein expression of the GluR1 AMPA receptor subunit in cultured neocortical neurons. In agreement, TGFα treatment decreased the B max of [ 3 H] AMPA binding and GluR1 mRNA levels. Immunocytochemistry revealed that the decrease in GluR1 was most pronounced in multipolar GABAergic neurons. To examine the physiological consequences, we recorded AMPA-evoked currents as well as miniature excitatory postsynaptic currents in morphologically identified putative GABAergic neurons in culture. Subchronic TGFα treatment decreased AMPA-triggered currents as well as the amplitude and frequency of miniature excitatory postsynaptic currents. An ErbB1 tyrosine kinase inhibitor, PD153035, inhibited the TGFα effect. Moreover, TGFα counteracted the neurotrophic activity of BDNF on AMPA receptor expression. Co-application of TGFα with BDNF blocked the BDNF-triggered up-regulation of AMPA receptor expression and currents. These observations reveal a negative regulatory activity of the ErbB1 ligand, TGFα, which reduces the input sensitivity of cortical GABAergic neurons to attenuate their inhibitory function.

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Section: Distinction Between Mitotic Activity and Ampa Receptor-reducsupporting

confidence: 93%

Section: Effect Of Tgfα On Glur1 Immunoreactivitymentioning

confidence: 86%

Section: Biological Implication Of Tgfα Actionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transforming growth factor alpha attenuates the functional expression of AMPA receptors in cortical GABAergic neurons

Namba

Nagano

Iwakura

et al. 2006

Molecular and Cellular Neuroscience

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“…EGFR expression also declines with the age of the animal in other parts of the CNS. For example, EGFR is expressed at relatively high levels until postnatal day 19 in both astrocytes and Purkinje cells, with levels of expression declining thereafter (Gomez-Pinilla et al, 1988). By contrast, EGFR persists into adulthood in neurogenic regions, such as the SVZ (Doetsch et al, 2002;Seroogy et al, 1995;Weickert et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Liu

Gümüşçü

et al. 2006

Glia

111

121

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Epidermal growth factor (EGF) is known to promote proliferation of both retinal progenitors and Muller glia in vitro, but several questions remain concerning an in vivo role for this factor. In this study, we investigated whether the EGF receptor (EGFR) is necessary for the maintenance of normal levels of progenitor and Muller glial proliferation in vivo. Here, we show that (1) mice with homozygous deletion of the Egfr gene have reduced proliferation in late stages of retinal histogenesis, (2) EGF is mitogenic for M€ uller glia in vivo during the first two postnatal weeks in the rodent retina, (3) the effectiveness of EGF as a M€ uller glial mitogen declines in parallel with the decline in EGFR expression as the retina matures, and (4) following damage to the retina from continuous light exposure, EGFR expression is upregulated in M€ uller glia to levels close to those in the neonatal retina, resulting in a renewed mitotic response to EGF. Together with previous results from other studies, these data indicate that the downregulation of a growth factor receptor is one mechanism by which glial cells maintain mitotic quiescence in the mature nervous system. V V C 2006 Wiley-Liss, Inc.

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Neuregulins and ErbB receptors in cultured neonatal astrocytes

et al. 1999

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Neuregulins (NRGs) are a family of growth factors involved in signaling between neurons and glial cells of the peripheral and central nervous system. NRGs are synthesized and secreted by a number of cell types including Schwann cells, neurons, and oligodendrocytes. NRG transduction signals are mediated by the erbB family of receptor tyrosine kinases. These NRGs may be important for paracrine or autocrine signaling during development, injury, and the normal functioning of the central nervous system. In this study, we characterize the NRGs and erbB receptors expressed by cultured neonatal rat astrocytes. Using immunoblotting protocols with pan-specific antibodies, we identified eleven NRG molecular weight isoforms from approximately 16 kDa to 105 kDa in cultured neonatal rat astrocytes. Immunocyotchemistry with isoform-specific antibodies revealed the expression of both major isoform families (NRGalpha, NRGbeta). Additionally, astrocyte-conditioned media contained two molecular weight isoforms of NRGs. We detected mRNA expression of NRGalpha and NRGbeta in astrocytes by amplifying mRNA transcripts with reverse transcription polymerase chain reaction. Furthermore, we confirm that cultured astrocytes express all four erbB receptors as detected by immunocytochemical and immunoblotting techniques. These data indicate that astrocytes contain and secrete NRGs.

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Epidermal growth factor receptor immunoreactivity in rat brain. Development and cellular localization

Cited by 170 publications

References 17 publications

Transforming growth factor alpha attenuates the functional expression of AMPA receptors in cortical GABAergic neurons

Transforming growth factor alpha attenuates the functional expression of AMPA receptors in cortical GABAergic neurons

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Neuregulins and ErbB receptors in cultured neonatal astrocytes

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