Epidermal Growth Factor–Releasing Radially Aligned Electrospun Nanofibrous Patches for the Regeneration of Chronic Tympanic Membrane Perforations

Seonwoo, Hoon; Shin, Beomyong; Jang, Kitae; Lee, Myung Chul; Choo, Oak‐Sung; Park, Sangbae; Kim, Yeong Cheol; Choi, Mijin; Kim, Jangho; Garg, Pankaj; Jang, Jeong Hun; Choung, Yun‐Hoon; Chung, Jong Hoon

doi:10.1002/adhm.201801160

Cited by 41 publications

(32 citation statements)

References 34 publications

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“…Therefore, CS/NaHCO 3 bioink is suitable for 3D bioprinting and tissue engineering applications. By adding other components [32], such as growth factors [33] and graphene [21], their functionality could be enhanced, thereby extending their potential applicability.…”

Section: Printing Cell-laden Constructsmentioning

confidence: 99%

Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Seonwoo

Park

et al. 2020

Applied Sciences

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Three-dimensional (3D) bioprinting is a technology used to deposit cell-laden biomaterials for the construction of complex tissues. Thermosensitive hydrogels are physically cross-linked by non-covalent interaction without using crosslinkers, facilitating low cytotoxicity and cell viability. Chitosan, which is a non-toxic, biocompatible and biodegradable polysaccharide, can be used as a thermosensitive hydrogel. Therefore, chitosan hydrogel could be of potential use as a 3D bioprinting ink. The purpose of this study was to develop and compare the effectivity of different bioinks based on chitosan hydrogels for 3D bioprinting. The solvent type did not affect the gel shape and gelation time, whereas acetic acid exhibited better biocompatibility compared to lactic and hydrochloric acids. The nature of the gelling agent was found to have a stronger influence on these characteristics than that of the solvent. The NaHCO3 moiety exhibited a higher growth rate of the storage modulus (G′) and a more irregular porous structure than that of the β-glycerophosphate (β-GP) and K2HPO4 groups. Cell viability, and live and dead assays, showed that the NaHCO3 group was more efficient for cell adhesion. The type of gelling agent did not lead to appreciable differences in cell-laden constructs. The NaHCO3 group was more amenable to bioprinting, compared to the β-GP and K2HPO4 groups. The chitosan hydrogel bioinks could, therefore, be good candidates for 3D bioprinting and would pave the way for patient-specific regenerative medicines.

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Section: Printing Cell-laden Constructsmentioning

confidence: 99%

Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Seonwoo

Park

et al. 2020

Applied Sciences

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate otologic tissue engineering 15,25‐35,38‐43,45,46,107‐144 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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“…Growth factors are biologically active polypeptides that are beneficial to control cell growth, proliferation, and migration during the wound healing process. All the stages of the wound healing process can be regulated using a wide variety of growth factors [90,91]. The role of vitamins in the healing procedure is also considerable.…”

Section: Electrospun Wound Dressings Loaded With Bioactive Moleculesmentioning

confidence: 99%

Bio-Based Electrospun Fibers for Wound Healing

Azimi

Maleki

Zavagna

et al. 2020

JFB

164

106

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Being designated to protect other tissues, skin is the first and largest human body organ to be injured and for this reason, it is accredited with a high capacity for self-repairing. However, in the case of profound lesions or large surface loss, the natural wound healing process may be ineffective or insufficient, leading to detrimental and painful conditions that require repair adjuvants and tissue substitutes. In addition to the conventional wound care options, biodegradable polymers, both synthetic and biologic origin, are gaining increased importance for their high biocompatibility, biodegradation, and bioactive properties, such as antimicrobial, immunomodulatory, cell proliferative, and angiogenic. To create a microenvironment suitable for the healing process, a key property is the ability of a polymer to be spun into submicrometric fibers (e.g., via electrospinning), since they mimic the fibrous extracellular matrix and can support neo- tissue growth. A number of biodegradable polymers used in the biomedical sector comply with the definition of bio-based polymers (known also as biopolymers), which are recently being used in other industrial sectors for reducing the material and energy impact on the environment, as they are derived from renewable biological resources. In this review, after a description of the fundamental concepts of wound healing, with emphasis on advanced wound dressings, the recent developments of bio-based natural and synthetic electrospun structures for efficient wound healing applications are highlighted and discussed. This review aims to improve awareness on the use of bio-based polymers in medical devices.

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Epidermal Growth Factor–Releasing Radially Aligned Electrospun Nanofibrous Patches for the Regeneration of Chronic Tympanic Membrane Perforations

Cited by 41 publications

References 34 publications

Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Tissue engineering applications in otolaryngology—The state of translation

Bio-Based Electrospun Fibers for Wound Healing

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