Epidermal growth factor system is a physiological regulator of development of the mouse fetal submandibular gland and regulates expression of the α6-integrin subunit

Kashimata, Masanori; Gresik, Edward W.

doi:10.1002/(sici)1097-0177(199702)208:2<149::aid-aja2>3.0.co;2-i

Cited by 86 publications

(74 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell-surface ␤1 integrin levels were unaffected by Lrig1 knockdown, but there was a small increase in ␣6␤4 (Fig. 3C), which might be attributable to increased EGFR1 signaling (30). Keratinocytes expressing Lrig1 siRNA showed a marked increase in growth rate (Fig.…”

Section: High Levels Of Lrig1 Reduce Keratinocyte Proliferation and Egfmentioning

confidence: 95%

Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence

Jensen

Watt²

2006

Proc. Natl. Acad. Sci. U.S.A.

298

310

View full text Add to dashboard Cite

Section: High Levels Of Lrig1 Reduce Keratinocyte Proliferation and Egfmentioning

confidence: 95%

Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence

Jensen

Watt²

2006

Proc. Natl. Acad. Sci. U.S.A.

298

310

View full text Add to dashboard Cite

“…Branching morphogenesis of ex vivo SMGs involves both FGFR signaling (Hoffman et al, 2002;Jaskoll et al, 2002;Morita and Nogawa, 1999) and EGFR signaling (Kashimata and Gresik, 1997;Kashimata et al, 2000). Previously, we identified a role for FGFR1 and phosphatidylinositol 3-kinase (PI3K) in SMG branching morphogenesis (Larsen et al, 2003).…”

Section: Introductionmentioning

confidence: 95%

FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis

et al. 2005

View full text Add to dashboard Cite

Branching morphogenesis of mouse submandibular glands is regulated by multiple growth factors. Here, we report that ex vivo branching of intact submandibular glands decreases when either FGFR2 expression is downregulated or soluble recombinant FGFR2b competes out the endogenous growth factors. However, a combination of neutralizing antibodies to FGF1, FGF7 and FGF10 is required to inhibit branching in the intact gland, suggesting that multiple FGF isoforms are required for branching. Exogenous FGFs added to submandibular epithelial rudiments cultured without mesenchyme induce distinct morphologies. FGF7 induces epithelial budding, whereas FGF10 induces duct elongation, and both are inhibited by FGFR or ERK1/2 signaling inhibitors. However, a PI3-kinase inhibitor also decreases FGF7-mediated epithelial budding, suggesting that multiple signaling pathways exist. We immunolocalized FGF receptors and analyzed changes in FGFR, FGF and MMP gene expression to identify the mechanisms of FGF-mediated morphogenesis. FGFR1b and FGFR2b are present throughout the epithelium,although FGFR1b is more highly expressed around the periphery of the buds and the duct tips. FGF7 signaling increases FGFR1b and FGF1expression, and MMP2 activity, when compared with FGF10, resulting in increased cell proliferation and expansion of the epithelial bud, whereas FGF10 stimulates localized proliferation at the tip of the duct. FGF7- and FGF10-mediated morphogenesis is inhibited by an MMP inhibitor and a neutralizing antibody to FGF1, suggesting that both FGF1 and MMPs are essential downstream mediators of epithelial morphogenesis. Taken together,our data suggests that FGFR2b signaling involves a regulatory network of FGFR1b/FGF1/MMP2 expression that mediates budding and duct elongation during branching morphogenesis.

show abstract

“…Branching morphogenesis of SMGs in vitro involves EGF signaling (Kashimata and Gresik, 1997;Kashimata et al, 2000;Morita and Nogawa, 1999), integrin α6, laminin-1 (Hosokawa et al, 1999;Kadoya et al, 1995), the TNF/TNFR1/IL6 pathways (Melnick et al, 2001b;Melnick et al, 2001c), and FGF7 (Morita and Nogawa, 1999). Fibroblast growth factors (FGFs) and their receptors (FGFRs) are important in many developmental events, including branching morphogenesis in other organs (Martin, 1998;Metzger and Krasnow, 1999;Ornitz, 2000;Spencer-Dene et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Gene expression profiles of mouse submandibular gland development: FGFR1 regulates branching morphogenesis in vitro through BMP- and FGF-dependent mechanisms

et al. 2002

View full text Add to dashboard Cite

Analyses of gene expression profiles at five different stages of mouse submandibular salivary gland development provide insight into gland organogenesis and identify genes that may be critical at different stages. Genes with similar expression profiles were clustered, and RT-PCR was used to confirm the developmental changes. We focused on fibroblast growth factor receptor 1 (FGFR1), as its expression is highest early in gland development. We

show abstract

Epidermal growth factor system is a physiological regulator of development of the mouse fetal submandibular gland and regulates expression of the α6-integrin subunit

Cited by 86 publications

References 59 publications

Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence

Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence

FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis

Gene expression profiles of mouse submandibular gland development: FGFR1 regulates branching morphogenesis in vitro through BMP- and FGF-dependent mechanisms

Contact Info

Product

Resources

About