Epididymal Sperm Granuloma and Antisperm Antibodies in Donkeys

Podico, Giorgia; Lago-Alvarez, Yamilka; Carossino, Mariano; Ferrer, Maria S.; Arnold, Carolyn E.; Canisso, Igor F.

doi:10.1016/j.jevs.2021.103450

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…The sperm membrane integrity and mitochondrial membrane potential were assessed pre-(0 h), 24 h, and 48 h post-cooling and after freezing and thawing [ 9 ]. The evaluation of the plasma membrane integrity and mitochondrial membrane potential was conducted using a spectral flow cytometer, as previously described [ 18 ] and as extensively used in the laboratory of the investigators of the present study [ 9 , 19 , 20 , 21 ]. Briefly, the staining solution of Zombie Green dye (#423112 BioLegend, San Diego, CA, USA) was freshly prepared with 100 μL of DMSO added to each vial of dye.…”

Section: Methodsmentioning

confidence: 99%

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Podico

Spencer

Magalhaes

et al. 2023

Veterinary Sciences

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This study aimed to assess the semen quality after the cooling and freezing of the first and second ejaculates of the season, which were collected 1 h apart. After collection (n = 40 ejaculates), the gel-free semen volume, concentration, total number of sperm, and sperm morphology were determined. An aliquot of each ejaculate was extended and cooled for 48 h; a second aliquot was cushion-centrifuged and cooled for 48 h; and a third aliquot was processed and then frozen. The total motility (TM) and progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP) were assessed pre-(0 h), 24 h, and 48 h post-cooling and before and after freezing. The second ejaculate had a lower gel-free semen volume (p = 0.026). The sperm concentration was greater in the first than in the second ejaculate (p < 0.001). The sperm morphology was similar between the ejaculates (p > 0.05). Cushion-centrifugation prevented a reduction in the TM, PM, and PMI over time (p < 0.05). The TM, PM, and PMI decreased after freezing but not between the ejaculates (p > 0.05). The first and second ejaculates of the season, which were collected 1 h apart, varied in quantity but not in quality after cooling and freezing.

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Section: Methodsmentioning

confidence: 99%

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Podico

Spencer

Magalhaes

et al. 2023

Veterinary Sciences

Self Cite

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Reproductive Disorders in Donkeys: Current Evidence and Update

Wang,

Zeng,

Wang

et al. 2024

Animals

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Reproductive disorders in donkeys present a significant challenge to their health and welfare, impacting their roles in agriculture, conservation, and companionship. With the development of large-scale donkey farming in recent years, reproductive disorders have become a limiting factor for the expansion of the donkey population. In general, donkeys suffer from a similar array of diseases like horses, but little is known about the specificities of donkey reproductive disorders. This review synthesizes current knowledge on the pathogenesis, distribution, presentation, diagnosis, treatment, and prognosis of a diverse array of reproductive disorders affecting donkeys. There are similar infectious and non-infectious causes for infertility and pregnancy loss in jennies compared with mares, but a difference in disease susceptibility does exist, which may be attributed to genetic influence, pathogen specificity, the environment, and reproductive management practices. Diagnostic and treatment plans need to be tailored towards the particularities of donkey reproductive disorders to increase donkey populations and to enhance the standard of care for this species. Enhancing reproductive health in donkeys not only ensures their sustainable use but also promotes their welfare and longevity in diverse human–animal interactions.

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Epididymal Sperm Granuloma and Antisperm Antibodies in Donkeys

Cited by 2 publications

References 38 publications

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Reproductive Disorders in Donkeys: Current Evidence and Update

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