Epigenetic Alteration in DNA methylation pattern and gene expression level using H19 on oligospermia patients in Iraqi Men

Al-Qazzaz, Halah Kamal; Al-Awadi, Salwa J.

doi:10.1016/j.genrep.2020.100780

Cited by 3 publications

(3 citation statements)

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“…Enzyme Linked Immunosorbent Assay (ELISA) test is a method of quantitative sandwich enzyme immunoassay, the microplate has been pre-coated with a monoclonal antibody (mAb) specific for human PCSK9 12 . The PCSK9 concentration in each serum sample was represented as ng/ml for the comparison with the results with those of controls concentration and the detection ranges: 177ng/ml-460ng/ml, ELISA kits used in this study produced by (R&D/ USA).…”

Section: Biochemical Analysismentioning

confidence: 99%

“…Sodium bisulfite was used to modify genomic DNA, converting all unmethylated cytosine to uracil, while methylated cytosine remain unchanged. After completion of the bisulfite conversion, the converted DNA is ready to be used in the HRM-PCR technique for determining the percentage of methylated and un-methylated cytosine in DNA 12 . A primer sequence utilized in the present study as shown in Moreover, the Pearson Correlation produces a correlation coefficient (r) which measures the relationships between the studied parameters.…”

Section: Dna Methylation Of Pcsk9 Genementioning

confidence: 99%

“…4 For PCSK9 methylation levels mean ± SD, a highly significant relationship between two study groups p=0.002, the mean for patients group 51.28±21.32 that leading to indicate that hypomethylation has happened, while in the controls group hypermethylation was happened 62.72±20.67. DNA methylation regulates gene transcription where DNA hypermethylation usually leads to a long-term and stable gene suppression and DNA hypomethylation of the gene leads to gene expression 12,32 . The results of recent study found that were patients with methylated PCSK9 promoter, the PCSK9 gene expression and serum circulating levels are lower in those with unmethylated DNA, indicating a lower risk of disease 11 .…”

Section: Dna Methylation Of Pcsk9 Using Highiresolutionimeltingianalysismentioning

confidence: 99%

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Gene Expression and Methylation Levels of PCSK9 Gene in Iraqi Patients with Coronary Artery Disease

2023

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The expression of the Proprotein Convertase Subtilisin/Kexin Type 9 gene (PCSK9) is inextricably related to lipid levels and a risk of atherosclerotic coronary artery disease (ASCAD). The present study aims to measure the quantity of PCSK9 gene expression and the effect of methylation on its expression level taking part in the pathogenesis of acute coronary artery disorder. A current study included 150 subjects from the Iraqi population, 100 ASCAD patients and 50 healthy controls. The concentration of PCSK9 in each serum sample was determined by the ELISA technique, the expression levels of the PCSK9 gene in whole blood were estimated by RT-qPCR – Quantitative Reverse Transcription PCR method, and DNA methylation level detection in PCSK9 gene by using High Resolution Melting Analysis (HRM) technique. The expression level of the PCSK9 gene was 6.06 ± 1.84 in ASCAD patients group compared with a control group 1.00 ±0.19. That indicates the up-regulation of the PCSK9 gene in patient with atherosclerosis. In addition, the PCSK9 concentration was higher in patient group in comparison to the healthy controls group P= < 0.01. However, PCSK9 methylation levels, a highly significant distinction between the two study groups p=0.002. In conclusion, serum PCSK9 levels are associated with coronary artery lesions as shown in receiver operating characteristic (ROC) analysis. Suggests that, serum PCSK9 might be a good indicator of coronary artery disease development.

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Section: Biochemical Analysismentioning

confidence: 99%

Section: Dna Methylation Of Pcsk9 Genementioning

confidence: 99%