Epigenetics in the plant–virus interaction

Wang, Chenguang; Wang, Chaonan; Zou, Jing-Ze; Yang, Yunshu; Li, Zhihong; Zhu, Shuifang

doi:10.1007/s00299-019-02414-0

Cited by 42 publications

(38 citation statements)

References 97 publications

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“…Plant genome methylation and viral methylation has been reported to be a new layer of epigenetic change in response to viral infection [ 39 ]. Methylation across genome of DNA viruses restricts their replication, unlike RNA viruses that do not use a DNA replication process [ 10 ]. RBSDV is a member of the genus Fijivirus with dsRNA as genome for replication, its infection results in severe yield losses in rice [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have provided evidence that DNA methylation modulates immune responses against plant viruses. The chromatin-like organisation of plant DNA viruses is more easily methylated to restrict their proliferation due to their single-stranded DNA (ssDNA) genome and double-stranded DNA (dsDNA) replication process [ 10 ]. Thus, viral genome methylation can be employed by plants as an epigenetic defense against DNA viruses.…”

Section: Introductionmentioning

confidence: 99%

“…Silencing of Nicotiana benthamiana histone deacetylase 6 ( NbHDA6 ) expression results in a reduced DNA methylation level of the viral genome and enhanced host susceptibility to TYLCV infection [ 12 ]. Unlike DNA viruses, RNA viruses possess scarcely methylated RNA genomes for replication [ 10 ]. DNA methylation regulates the expression of plant disease resistance genes to respond to the infection of RNA viruses [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus

Zhang

et al. 2020

IJMS

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Rice black-streaked dwarf virus (RBSDV) causes severe yield losses in rice (Oryza sativa L.) in China. Studies have shown that the mechanisms of DNA methylation-mediated plant defense against DNA viruses and RNA viruses are different. However, in rice its function in response to infection of RBSDV, a double-stranded RNA virus, remains unclear. In this study, high-throughput single-base resolution bisulfite sequencing (BS-Seq) was carried out to analyze the distribution pattern and characteristics of cytosine methylation in RBSDV-infected rice. Widespread differences were identified in CG and non-CG contexts between the RBSDV-infected and RBSDV-free rice. We identified a large number of differentially methylated regions (DMRs) along the genome of RBSDV-infected rice. Additionally, the transcriptome sequencing analysis obtained 1119 differentially expressed genes (DEGs). Correlation analysis of DMRs-related genes (DMGs) and DEGs filtered 102 genes with positive correlation and 71 genes with negative correlation between methylation level at promoter regions and gene expression. Key genes associated with maintaining DNA methylation in rice were analyzed by RT-qPCR and indicated that OsDMT702 might be responsible for the global increase of DNA methylation level in rice under RBSDV stress. Our results suggest important roles of rice DNA methylation in response to RBSDV and provide potential target genes for rice antiviral immunity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus

Zhang

et al. 2020

IJMS

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“…Cytosine DNA methylation, one of the fundamental aspects of stable epigenetic modification, modulates coding genes that involve various cellular biological processes and molecular functions to counter virus infection (Wang et al, 2019). The cytosines in the genome of DNA viruses could be directly methylated through plant DNA methylation; for example, geminivirus transcription is usually disrupted by host DNA methylation, which occurs in the viral genome through RNAdirected DNA methylation (RdDM) (Coursey et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The NIa-Protease Protein Encoded by the Pepper Mottle Virus Is a Pathogenicity Determinant and Releases DNA Methylation of Nicotiana benthamiana

et al. 2020

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It is well documented that the canonical function of NIa-protease (NIa-Pro) of the potyviruses is responsible for cleaving the viral polyprotein into functional proteins. Although NIa-Pro is vital for the infection cycle of potyviruses, the function of NIa-Pro in the interaction of the potyvirus host is not clear. In this study, NIa-Pro is ectopically expressed from a potato virus X (PVX) vector and infiltrates Nicotiana benthamiana wild type and 16-TGS. The pathogenicity and inhibition of host transcriptional gene silencing (TGS) are characterized. Ectopic expression of NIa-Pro from a PVX vector resulted in severe mosaic symptoms followed by a hypersensitive-like response in N. benthamiana. Furthermore, PepMoV NIa-Pro was able to reverse established TGS of a green fluorescent protein transgene by reducing methylation of promoter sequences in N. benthamiana and possessed the capacity to interfere with the global methylation of N. benthamiana. Taken together, the results of this study likely suggest that PepMoV NIa-Pro is a pathogenicity determinant and a potent suppressor of host TGS and suggest that NIa-Pro may employ novel mechanisms to suppress host antiviral defenses. To the best of our knowledge, this is the first report of a plant RNA virus modulating host TGS in a novel manner by interfering with the establishment of the methylation step of the plant DNA methylation pathway.

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“…In turn, that dsRNA can produce secondary vsiRNAs by DCLs activity, amplifying the antiviral defense (Ding, 2010;Carbonell and Carrington, 2015). In the last years, several authors reported a close relationship between epigenetic modifications -DNA and histone methylation, mainly -involved in the transcriptional gene silencing and plant viral infections (Wang et al, 2019a). The RNA-dependent DNA methylation (RdDM), which was thoroughly described in Arabidopsis (Zhang et al 2018), is also mediated by siRNAs and produces de novo DNA methylation (Matzke and Mosher, 2014).…”

Section: Indirect M 6 A-mediated Viral Regulationmentioning

confidence: 99%

Involvement of the host RNA N6-adenosine methylation (m6A) pathway in the infection cycle of Alfalfa mosaic virus

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Epigenetics in the plant–virus interaction

Cited by 42 publications

References 97 publications

Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus

Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus

The NIa-Protease Protein Encoded by the Pepper Mottle Virus Is a Pathogenicity Determinant and Releases DNA Methylation of Nicotiana benthamiana

Involvement of the host RNA N6-adenosine methylation (m6A) pathway in the infection cycle of Alfalfa mosaic virus

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