Epilepsy Surgery: Current Status and Ongoing Challenges

Kawai, Kensuke

doi:10.2176/nmc.ra.2014-0414

Cited by 18 publications

(7 citation statements)

References 66 publications

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“…Patients with TLE suffer from consciousness and memory impairment [2] and psychosocial difficulties [3]. Epilepsy surgery and antiepileptic drugs are the major treatments for epileptic disorders [4]. However, both methods have side effects such as nausea, drowsiness, headache, dizziness, and ataxia [5].…”

Section: Introductionmentioning

confidence: 99%

Antagomirs Targeting MicroRNA-134 Increase Limk1 Levels After Experimental Seizures in Vitro and in Vivo

Sun

Gao

Meng

et al. 2017

Cell Physiol Biochem

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Background: MiR-134 is enriched in dendrites of hippocampal neurons and plays crucial roles in the progress of epilepsy. The present study aims to investigate the effects of antagomirs targeting miroRNA-134 (Ant-134) on limk1 expression and the binding of miR-134 and limk1 in experimental seizure. Methods: Status epilepticus (SE) rat model was established by lithium chloride-pilocarpine injection and was treated with Ant-134 by intracerebroventricular injection. Low Mg²⁺-exposed primary neurons were used as an in vitro model of SE. The expression of miR-134 was determined using real-time PCR. Protein expressions of limk1 and cofilin were determined by Western blotting. Luciferase reporter assay was used to examine the binding between miR-134 and limk1 3’-untranslated region. Results: The expression of miR-134 was markedly enhanced in hippocampus of the SE rats and low Mg²⁺-exposed neurons. Ant-134 increased the expression of limk1 and reduced the expression of cofilin in the SE hippocampus and Low Mg²⁺-exposed neurons. In addition, luciferase reporter assay confirmed that miR-134 bound limk1 3’-UTR. MiR-134 overexpression inhibited limk1 mRNA and protein expressions in neurons. Conclusion: Blockage of miR-134 upregulates limk1 expression and downregulated cofilin expression in hippocampus of the SE rats. This mechanism may contribute to the neuroprotective effects of Ant-134.

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Section: Introductionmentioning

confidence: 99%

Antagomirs Targeting MicroRNA-134 Increase Limk1 Levels After Experimental Seizures in Vitro and in Vivo

Sun

Gao

Meng

et al. 2017

Cell Physiol Biochem

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“…Patients with epilepsy are generally impaired by intermittent infuriation in movement, feeling, or consciousness which is caused by the suddenly synchronous and excessive discharges in cortical neurons ( Brodie and French, 2000 ). Currently, resective surgeries for focal epilepsy and new-generation antiepileptic drugs (AEDs) are the major treatments for patients with epilepsy ( Kawai, 2015 ). However, both methods have some following side effects such as headache, dizziness, fatigue, and ataxia ( Brodie and Dichter, 1996 ).…”

Section: Introductionmentioning

confidence: 99%

Antagonist Targeting microRNA-155 Protects against Lithium-Pilocarpine-Induced Status Epilepticus in C57BL/6 Mice by Activating Brain-Derived Neurotrophic Factor

Cai

et al. 2016

Front. Pharmacol.

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Epilepsy is a severe brain disorder affecting numerous patients. Recently, it is inferred that modulation of microRNA-155 (miR-155) could serve as a promising treatment of mesial temporal lobe epilepsy. In the current study, the therapeutic potential of miR-155 antagonist against temporal lobe epilepsy (TLE) was evaluated and the underlying mechanism involved in this regulation was explored. TLE model was induced by lithium-pilocarpine method. The effect of miR-155 antagonist on epilepticus symptoms of TLE mice was assessed using Racine classification and electroencephalogram (EEG) recordings. The expression of brain-derived neurotrophic factor (BDNF) and its association with miR-155 were also assessed with a series of experiments. Our results showed that level of miR-155 was significantly up-regulated after induction of TLE model. Based on the results of EEG and behavior analyses, seizures in mice were alleviated by miR-155 antagonist. Moreover, administration of miR-155 antagonist also significantly increased the level of BDNF. The results of dual luciferase assay and Western blotting showed that miR-155 antagonist exerted its action on status epilepticus by directly regulating the activity of BDNF. Taken all the information together, our results demonstrated that miR-155 antagonist might firstly induce the expression of BDNF, which then contributed to the alleviation of epilepsy in the current study.

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“…Therefore, a successful outcome depends greatly on the teams’ willingness to identify suitable candidates with an epileptogenic zone that is clearly established using locally available equipment and knowledge without risking patient safety 36 , 37 . This also involves acquiring knowledge on when not to perform the operation and requesting additional evaluation, which is important in the identification of beneficiaries with the available resources 38 .…”

Section: Discussionmentioning

confidence: 99%

Initiating an epilepsy surgery program with limited resources in Indonesia

Arifin

Hanaya

Bakhtiar

et al. 2021

Sci Rep

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To share the experiences of organizing the epilepsy surgery program in Indonesia. This study was divided into two periods based on the presurgical evaluation method: the first period (1999–2004), when interictal electroencephalogram (EEG) and magnetic resonance imaging (MRI) were used mainly for confirmation, and the second period (2005–2017), when long-term non-invasive and invasive video-EEG was involved in the evaluation. Long-term outcomes were recorded up to December 2019 based on the Engel scale. All 65 surgical recruits in the first period possessed temporal lobe epilepsy (TLE), while 524 patients were treated in the second period. In the first period, 76.8%, 16.1%, and 7.1% of patients with TLE achieved Classes I, II, and III, respectively, and in the second period, 89.4%, 5.5%, and 4.9% achieved Classes I, II, and III, respectively, alongside Class IV, at 0.3%. The overall median survival times for patients with focal impaired awareness seizures (FIAS), focal to bilateral tonic–clonic seizures and generalized tonic–clonic seizures were 9, 11 and 11 years (95% CI: 8.170–9.830, 10.170–11.830, and 7.265–14.735), respectively, with p = 0.04. The utilization of stringent and selective criteria to reserve surgeries is important for a successful epilepsy program with limited resources.

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Epilepsy Surgery: Current Status and Ongoing Challenges

Cited by 18 publications

References 66 publications

Antagomirs Targeting MicroRNA-134 Increase Limk1 Levels After Experimental Seizures in Vitro and in Vivo

Antagomirs Targeting MicroRNA-134 Increase Limk1 Levels After Experimental Seizures in Vitro and in Vivo

Antagonist Targeting microRNA-155 Protects against Lithium-Pilocarpine-Induced Status Epilepticus in C57BL/6 Mice by Activating Brain-Derived Neurotrophic Factor

Initiating an epilepsy surgery program with limited resources in Indonesia

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