Epinephrine‐modified methacrylate monolithic column with fumed silica nanoparticle for pressurized capillary electrochromatography

Abstract

Via the ring‐opening reaction of epoxy groups with epinephrine, a novel epinephrine functionalized polymethacrylate monolith with fumed silica nanoparticles has been fabricated for pressurized capillary electrochromatography. The preparation of epinephrine‐modified monoliths has been optimized. In addition, morphology, electroosmotic flow, separation mechanism and column performance have been studied. The internal structure of the monolithic stationary phase was more uniform and the column efficiency increased after the incorporation of nanoparticles. With this column, satisfactory separation capability of aromatic compounds and alkaloids has been achieved and the column efficiency for naphthalene reached 138 696 plates/m. As for the real sample, 3 alkaloids were separated in Huanglian Shangqing capsules, a Chinese traditional medicine.

Advances and strategies for capillary electrophoresis in the characterization of traditional Chinese medicine: A review of the past decade (2011–2021)

“…Nanoparticle-based monoliths have been introduced for the chromatographic separation of various solutes using CEC and HPLC (Chambers et al, 2011;Mayadunne and El Rassi, 2014). Nanoparticles can be included in monolith supports by attaching them to the pore surface or copolymerizing them with the monomers (Pan et al, 2019). However, the latter is the more accessible and more convenient technique.…”

“…The preparation of epinephrine-modified monoliths has been optimized for EOF and column performance. The proposed method showed satisfactory results in separating aromatic compounds and alkaloids (palmatine, jatrorrhizine, and berberine) in Huanglian Shangqing capsules (Pan et al, 2019). A novel and sensitive CEC approach for stereoisomer separation were developed by Ma et al (2016).…”

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

“…Fumed silica nanoparticles (FSNPs), with an average primary particle size of 12 nm and SBET = 200 ± 25 m 2 /g, are relatively new and attractive materials for CEC analysis, owing to their large contact surface area, amorphocity, and ease of modification . For example, Pan and co‐workers prepared a hybrid poly(glycidyl methacrylate‐coethylen glycol dimethacrylate‐methacryloyl fumed silica nanoparticles) monolithic column for CEC analysis. The column efficiency reached 138 696 plates/m.…”