Epinephrine quantification in pharmaceutical formulations utilizing plant tissue biosensors

Felix, Fabiana S.; Yamashita, Miyuki; Angnes, Lúcio

doi:10.1016/j.bios.2005.10.025

Cited by 53 publications

(33 citation statements)

References 36 publications

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“…The amperometric response of adrenaline was carried out at -0.23 V (vs. Ag/AgCl) via the electrochemical reduction of the product of the enzymatic reaction. Felix 13 have developed a biosensor to determine adrenaline in pharmaceutical samples. The polyphenol oxidase enzymes present in the fibers of palm tree fruits (Livistona chinensis) catalyze the oxidation of adrenaline to adrenaline-quinone as a primary product.…”

Section: Introductionmentioning

confidence: 99%

Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation

Franzoi¹,

Vieira²,

Dupont³

2010

J. Braz. Chem. Soc.

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Biossensores contendo lacase (Aspergillus oryzae) e líquidos iônicos derivados do cátion 1-butil-3-metilimidazol (BMI) associados com os ânions hexafluorfosfato (BMI•PF 6 ) ou bis(trifluormetilsulfonil)imida (BMI•Tf 2 N) foram construídos para determinação de adrenalina. O biossensor baseado no BMI•Tf 2 N foi selecionado por apresentar maior resposta quando comparado ao BMI•PF 6 . As melhores condições para otimização foram estabelecidas por voltametria de onda quadrada (amplitude 100 mV, frequência 10 Hz e incremento 4,0 mV). . The recovery of adrenaline in injectable samples ranged from 96.3 to 101.6%. The results obtained for the adrenaline using the proposed biosensor and the United States Pharmacopeia procedure were in agreement at the 95% confidence level.

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Section: Introductionmentioning

confidence: 99%

Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation

Franzoi¹,

Vieira²,

Dupont³

2010

J. Braz. Chem. Soc.

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“…Epinephrine (EP) or adrenaline, 1-(3, 4-dihydroxyphenyl)-2-methyloamino-ethanol, belongs to the catecholamine family and plays an important role as neurotransmitters and hormones (1). It is biosynthesized in the adrenal medulla and sympathetic nerve terminals, as well as beings secreted by the suprarenal gland along with norepinephrine.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the determination of EP in biological fl uids or pharmaceutical preparation is very important. Diff erent methods have been reported to determine EP, such as ultraviolet and visible spectrophotometry (UVvis) (4,5), fl uorimetry (6), liquid chromatographic liquid (7), capillary electrophoresis (8,9), amperometric (10), biamperometry (11), piezoelectric detection (12) and various sensors (1,13).…”

Section: Introductionmentioning

confidence: 99%

Sensitive determination of epinephrine in pharmaceutical preparation by flow injection coupled with chemiluminescence detection and mechanism study

Liu

Shi

2011

Luminescence

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A novel, rapid and sensitive method was described for the determination of epinephrine (EP) using flow injection analysis coupled with chemiluminescence (CL) detection, which based on EP enhanced the weak CL emission of luminol-KIO(4) system in NaOH solution. Parameters affecting the CL intensity and reproducibility were optimized systematically. Under the optimized experiment conditions, the net CL intensity was proportional to the concentration of EP in the range of 5.0 × 10(-8) to 1.5 × 10(-6) mol/L with a detection limit of 1.9 × 10(-9) mol/L. The relative standard deviation (RSD) was found to be 0.7% for 13 replicate determinations of 3.0 × 10(-7) mol/L EP. The applicability of the proposed method was illustrated in the determination of EP in pharmaceutical preparation. The recoveries of EP at different levels in EP hydrochloride injection were between 95.4 and 104.7%. One assay procedure takes only 27 s, and the sampling rate was calculated about to be 130 samples/h. The possible mechanism of the enhanced CL intensity was studied by examining CL spectra and UV-vis spectra.

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“…The biosensor is one of the methods used for adrenaline determination, which has good stability, low construction and storage cost, potential for miniaturization, automation simplicity, and construction portability [7,17,18].…”

Section: Introductionmentioning

confidence: 99%

Enzyme from Banana (<i>Musa</i> sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction

Silva¹,

Wisniewski²,

Luccas³

et al. 2013

AJAC

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For building a biosensor, at least two enzyme sources can be employed. The pure enzyme has features with better selectivity and low stability. Crude extract presents better stability and wrong selectivity. Thus, one intermediate condition can be feasible joining both benefits of crude extract and pure enzymes. For that result, several procedures of extraction and semi purification of polyphenol oxidase (PPO) enzyme from banana (Musa sp.) were studied. The results showed that cleaned enzymatic extracts presented higher specific activities than crude extracts (2.8 up to 5.3 fold), despite the total protein concentration diminishing from 27% up to 72%, indicating that polyphenol oxidase (PPO) enzyme was preserved in them. The biosensors with 125 AU mL -1 containing cleaned enzymatic extracts performed better by grinding or grinding plus sonication for 30 s. They were linear over the ranges of 5.9 × 10 -6 mol·L -1 to 1.4 × 10 -3 mol·L -1 and 7.9 × 10 -8 mol·L -1 to 4.0 × 10 -3 mol·L -1 , respectively. The limit of Detection (LOD) was 5.9 × 10 -6 mol·L -1 and 7.9 × 10 -8 mol·L -1 , respectively. The LOD obtained is adequate to adrenaline determination on blood and medicinal samples, and were applied in medicinal samples with satisfactory results.

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Epinephrine quantification in pharmaceutical formulations utilizing plant tissue biosensors

Cited by 53 publications

References 36 publications

Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation

Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation

Sensitive determination of epinephrine in pharmaceutical preparation by flow injection coupled with chemiluminescence detection and mechanism study

Enzyme from Banana (<i>Musa</i> sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction

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Epinephrine quantification in pharmaceutical formulations utilizing plant tissue biosensors

Cited by 53 publications

References 36 publications

Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation

Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation

Sensitive determination of epinephrine in pharmaceutical preparation by flow injection coupled with chemiluminescence detection and mechanism study

Enzyme from Banana (&lt;i&gt;Musa&lt;/i&gt; sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction

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Product

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Enzyme from Banana (<i>Musa</i> sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction