Epitachophoresis is a novel versatile total nucleic acid extraction method

Abstract

Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.

Extracting high-molecular weight DNA from cyanobacteria using Promega's WizardⓇ HMW DNA extraction kit with a modified protocol, METIS

“…Isolating HMW DNA from Gram-Positive and Gram-Negative Bacteria” [4] . It is not uncommon for researchers to modify methods or generate new methods to produce HMW DNA for sequencing and genetic analysis [5 , 6] . To the best of our knowledge, there is no known commercial kit with a protocol that is specific for cyanobacteria.…”

“…Some methods have explored the combined use of enzymatic and freeze thaw disruption with CTAB precipitation, and have been successful in HMW DNA extraction [11] . A recent study testing several DNA extraction kits (such as Qiagen QIAamp and DNeasy Plant mini-kits and Mo Bio Power Biofilm), were shown to be superior to phenol-chloroform methods [5] . Also, an earlier modified extraction method was successful in acquiring HMW DNA for cyanobacteria but can have some fragmentation [12] .…”

Epitachophoresis – A Method for the Extraction of Biomacromolecules from Complex Biological Matrices

“…ETP již byla popsána v několika našich studiích [4][5][6][7] . Jedná se o preparativní separační metodu založenou na diskontinuální elektroforéze umožňující koncentrovat a přečistit roztoky biomakromolekul z velkých objemů (až desítky ml) biologických matric (viz obr.…”

“…Naše dříve dosažené výsledky zveřejněné v pracích [4][5][6][7] jednoznačně dokazují, že ETP by mohla být důležitou technikou pro získávání proteinů a DNA z biologických matric. Umožňuje podstatné zakoncentrování cílových biomolekul a zároveň je schopná převést vzorek do čistší-ho roztoku.…”

3D-printed device for DNA fragmentation prior liquid biopsy testing

“…For that reason, the isolation of ctDNA fragments can enhance the performance of the subsequent PCR. 5,6 In our previous studies [7][8][9][10][11] , electromigration separation techniques proved to be a powerful tool for the extraction of biomacromolecules from complex biological matrices. Other examples of previously published systems for DNA (or other biomacromolecules) fragmentation and purification such as a preparative system from BIO-RAD laboratories (Hercules, USA), GELFREE 8100 Fractionation System (Expedeon Ltd, Cambridge, UK), commercially available cutters for DNA excision (NIPPON Genetics, Tokyo, Japan), and purification kits from Thermo Fischer Scientific (Waltham, USA), can be also mentioned.…”