Epithelial analysis of simple limbal epithelial transplantation in limbal stem cell deficiency by in vivo confocal microscopy and impression cytology

Prabhasawat, Pinnita; Luangaram, Angkoon; Ekpo, Pattama; Lekhanont, Kaevalin; Tangpagasit, Wimolwan; Boonwong, Chawikan; Inthasin, Naharuthai; Chirapapaisan, Chareenun

doi:10.1007/s10561-018-09746-3

Cited by 16 publications

(24 citation statements)

References 26 publications

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“…These included the largest prospective study to date, with 125 patients and a follow‐up period of at least 1 year and a multicenter international study of 68 patients . The remaining 23 studies were noncomparative single case studies or case series of 10 eyes or less . One study directly compared SLET with CLAU, with 10 patients randomly assigned to each group .…”

Section: Characteristics Of Slet Studiesmentioning

confidence: 99%

“…The Sangwan group in India published the most studies . Other centers in India, England, Brazil, Mexico, Thailand, and the United States also contributed.…”

Section: Characteristics Of Slet Studiesmentioning

confidence: 99%

“…Bilateral LSCD often occurs secondary to Stevens‐Johnson syndrome, mucous membrane phemigoid (MMP), and severe chemical burns, which produce extensive cicatrization or dryness making patients unsuitable candidates for treatment with SLET . A total of 56 eyes in six separate studies have used alloSLET . Immunosuppressant steroids were prescribed topically (19 eyes), systemically (1 eye), or in combination (30 eyes).…”

Section: Allogenic Sletmentioning

confidence: 99%

“…27 The remaining 23 studies were noncomparative single case studies or case series of 10 eyes or less. 2,4,[33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52] One study directly compared SLET with CLAU, with 10 patients randomly assigned to each group. 33 The Sangwan group in India published the most studies.…”

Section: Characteristics Of Slet Studiesmentioning

confidence: 99%

“…23 A total of 56 eyes in six separate studies have used alloSLET. 23,30,38,40,47,50 30 Ten of the patients were children with an age range of 3 months to 10 years. Systemic immunosuppressants were not used since later rejection of allogenic transplants was expected.…”

Section: Postoperativementioning

confidence: 99%

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Simple limbal epithelial transplantation: Current status and future perspectives

Jackson

Ernø

Ringstad

et al. 2019

Stem Cells Translational Medicine

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Damage to limbal stem cells as a result of injury or disease can lead to limbal stem cell deficiency (LSCD). This disease is characterized by decreased vision that is often painful and may progress to blindness. Clinical features include inflammation, neovascularization, and persistent cornea epithelial defects. Successful strategies for treatment involve transplantation of grafts harvested from the limbus of the alternate healthy eye, called conjunctival‐limbal autograft (CLAU) and transplantation of limbal cell sheets cultured from limbal biopsies, termed cultured limbal epithelial transplantation (CLET). In 2012, Sangwan and colleagues presented simple limbal epithelial transplantation (SLET), a novel transplantation technique that combines the benefits of CLAU and CLET and avoids the challenges associated with both. In SLET a small biopsy from the limbus of the healthy eye is divided and distributed over human amniotic membrane, which is placed on the affected cornea. Outgrowth occurs from each small explant and a complete corneal epithelium is typically formed within 2 weeks. Advantages of SLET include reduced risk of iatrogenic LSCD occurring in the healthy cornea at harvest; direct transfer circumventing the need for cell culture; and the opportunity to perform biopsy harvest and transplantation in one operation. Success so far using SLET is comparable with CLAU and CLET. Of note, 336 of 404 (83%) operations using SLET resulted in restoration of the corneal epithelium, whereas visual acuity improved in 258 of the 373 (69%) reported cases. This review summarizes the results of 31 studies published on SLET since 2012. Progress, advantages, challenges, and suggestions for future studies are presented.

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Section: Characteristics Of Slet Studiesmentioning

confidence: 99%

“…The Sangwan group in India published the most studies . Other centers in India, England, Brazil, Mexico, Thailand, and the United States also contributed.…”

Section: Characteristics Of Slet Studiesmentioning

confidence: 99%