Epithelial-mesenchymal transition in main types of gastric carcinoma

Василенко, И В; Kondratyk, R.B.; Grekov, Ilya S.; Yarkov, A.M.

doi:10.31088/cem2021.10.2.13-20

Cited by 5 publications

(1 citation statement)

References 26 publications

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“…Также наблюдается потеря и другого цитокератина (цитокератин 18), который служит мишенью фактора транскрипции Snail и реализует многие молекулярно-генетические пути ЭМТ, в том числе подавляет синтез Е-кадгерина [3,7]. В таком случае одновременно можно видеть и обособление клеток с приобретением ими вытянутой фибробластоподобной формы и снижение и/или полную потерю синтеза цитокератина 18.…”

Section: результаты и обсуждениеunclassified

Microenvironment influence on the development of epithelial-mesenchymal transformation in lung cancer

Kondratyk

Grekov

Seleznev³

2022

Vestn. Ross. univ. družby nar., Ser. Med.

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Relevance. Epithelial-mesenchymal transformation (EMT) largely determines the biological behavior and prognosis of cancers of various localizations. It is known that the determining role in the control and implementation of the transition of the epithelial phenotype to the mesenchymal belongs to the microenvironment. At the same time, the histochemical and microscopic characteristics of stromal elements remain unclear; therefore, the aim of our study was to establish the morphological features of the stroma that affect the development of EMT in lung cancer. Materials and Methods. We studied 32 cases of lung cancer with hematoxylin and eosin staining of sections, Alcian blue at pH 1.0 and 2.5, PAS reaction, as well as immunohistochemical studies with monoclonal antibodies to HMW, AE 1 / AE 3, cytokeratin 18, c-erb B 2, vimentin E-cadherin, alpha-smooth muscle actin, GFAP and chromogranin A, Ki-67. In 16 cases, there was widespread epithelial-mesenchymal transformation (EMT), in 14 cases - focal and in 2 cases EMT was absent. In half of the cases of widespread EMT, it was complete; among the cases of focal EMT, it was not complete. The transition from the epithelial to the mesenchymal phenotype was facilitated by the activation of the stroma, namely, the presence of myofibroblasts and alcianophilia of the extracellular matrix, inflammatory cell infiltration, expression of the epidermal growth factor receptor (c-erb B 2) in stromal cells, proliferation and neurogenic differentiation of stromal cells of stromal cells. Results and Discussion. The activated stroma correlates with the frequency of EMT. Thus, large areas of activated stroma with the presence of myofibroblasts and alcianophilia of the extracellular matrix are more common in cases of widespread EMT (14 out of 16-87.5 %) than in cases of focal EMT (4 out of 14-28.6 %). The differences are statistically significant, p 0.01. Inflammatory cell infiltration, which is the source of a signal for transformation, expression of the epidermal growth factor receptor (c-erb B 2) in stromal cells, proliferation, and neurogenic differentiation of stromal cells also correlated with the frequency of EMT. In all cases, the differences are statistically significant, p0.01. Conclusions. The data obtained indicate the undoubted influence of signals from the activated stroma on the development of epithelial-mesenchymal transformation of tumor cells.

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Section: результаты и обсуждениеunclassified

Microenvironment influence on the development of epithelial-mesenchymal transformation in lung cancer

Kondratyk

Grekov

Seleznev³

2022

Vestn. Ross. univ. družby nar., Ser. Med.

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The significance of the tumor microenvironment and molecular biological parameters in assessing the prognosis of the disease in patients with early-stage gastric cancer

Rumyantseva¹,

Avdalyan²,

Ivanov³

et al. 2023

Onkol. Z. im. P.A. Gercena

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The features of enzymes activity to nucleoside and antioxidant systems in solid tumors

Bakurova

Василенко

Tursunova

et al. 2022

Rossijskij bioterapevtičeskij žurnal

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Background. Solid tumors can create their aggressive properties. There are characterized by the invasion and metastatic activity, the resistance of the tumor clone to apoptosis. These pathways triggering may be realized both by 2-deoxy-D-ribose and its phosphorylated form (2-d-D-Rib-1-P), and by hydrogen peroxide.Aim. To investigate the peculiarities of enzymes activity to nucleoside metabolism and antioxidant system in epithelial tumors of different localization.Materials and methods. The features of the thymidine phosphorylase, adenosine deaminase, superoxide dismutase (SOD) and glutathione peroxidase (GPO) activity were studied in tumor homogenates. The visually no transformed tissues of the surgical resection edges were used as a control. Enzymes activity was determined by spectrophotometrical and morphological features were examined by the immunohistochemical methods in tissues of non-small cell lung cancer (NSCLC), samples of gastric and colon carcinomas (GCC).Results. Thymidine phosphorylase activity and adenosine deaminase activity in various malignant tumors were increased in comparison to the control. Respectively, thymidine phosphorylase activity was higher than by 1.8 times (p = 0.002 for NSCLC, p = 0.001 for GCC). An increase of adenosine deaminase activity was revealed both in tissues of NSCLC (more than 1.7 times) and in GCC (by 1.9 times, p = 0.001). No significant changes in SOD activity were detected in the tumors. GPO activity tended to decrease by an average of 1.3 times (p = 0.01 for NSCLC, p = 0.02 for GCC). A cluster analysis of the enzymatic activity features of the studied NSCLC tumors, as well as GCC, revealed their metabolic heterogeneity. According to its results, tumors of different localization were distinguished into 2 clusters. Common feature to their second clusters was an increase the SOD activity. It was accompanied by increase of thymidine phosphorylase activity (p = 0.045 for NSCLC, p = 0.049 for GCC). Therefore, both hydrogen peroxide and 2-d-D-Rib-1-P could be formed in them more intensively. It is important to note that morphological indicators of tumor aggression (decreased or lost expression of cell-cell adhesion marker, expression of mesenchymal markers, active angiogenesis) were detected more frequently in these subgroups.Conclusion. The obtained results reveal that individual features of the enzymes activity in epithelial tumors may be available source of 2-d-D-Rib-1-P and hydrogen peroxide generation in human cancer cells. In the case of individual higher tumor activity of thymidine phosphorylase and SOD and low GPO activity the metabolic stimulation of tumor progression may be occur.

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Epithelial-mesenchymal transition in main types of gastric carcinoma

Cited by 5 publications

References 26 publications

Microenvironment influence on the development of epithelial-mesenchymal transformation in lung cancer

Microenvironment influence on the development of epithelial-mesenchymal transformation in lung cancer

The significance of the tumor microenvironment and molecular biological parameters in assessing the prognosis of the disease in patients with early-stage gastric cancer

The features of enzymes activity to nucleoside and antioxidant systems in solid tumors

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