Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response

Feng, Zunyong; Zhou, Jing; Liu, Yinhua; Xia, Ruixue; Li, Qiang; Yan, Liang; Chen, Qun; Chen, Xiaobing; Jiang, Yuxin; Gao, Chao; Wang, Ming; Zhou, Guoren; Zhang, Yijie; Wang, Yongsheng; Xia, Hongping

doi:10.1038/s41418-021-00750-x

Cited by 56 publications

(43 citation statements)

References 60 publications

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“…Both pro-inflammatory cytokines and ROS are potential activators of nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome, which is consistent of NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC) and caspase-1, and promotes the maturation and release of pro-inflammatory cytokines (e.g., IL-1β and IL-18) [ [7] , [8] , [9] , [10] ]. Macrophages are the dominant inflammatory cells in the lungs, and play critical roles in sepsis-induced inflammation, oxidative stress and ALI [ 11 ]. Hence, targeting macrophages may develop novel therapeutic strategies against sepsis-induced ALI.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Macrophage SAMSN1 protects against sepsis-induced acute lung injury in mice

Jiang

Bai

et al. 2022

Redox Biology

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Macrophage SAMSN1 protects against sepsis-induced acute lung injury in mice

Jiang

Bai

et al. 2022

Redox Biology

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“…RGS1 is currently characterized as a proinflammatory regulator that is correlated with the poor differentiation and prognosis of several tumors (12)(13)(14)(15)(16). RGS1 is up-regulated to promote melanoma progression through phosphorylation of protein kinase B (AKT) and extracellular regulated protein kinases (ERK) (10); RGS1 can also regulate the immunophenotype of alveolar macrophage subpopulations, including inflammatory response, injury repair, and fibrosis phenotypes, via intracellular calcium (Ca) 2+ regulation (17).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma

Zhang¹,

Meng²,

Ma³

et al. 2021

Ann Transl Med

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Background: To investigate the role of microRNA-376b-3p (miR-376b-3p) and regulator of G protein signaling 1 (RGS1) in the proliferation, metastasis, and apoptosis of osteosarcoma.Methods: Differentially expressed genes (DEGs) between tumor and normal tissues from GSE14359 and GSE33382 in the cancer genome atlas (TCGA) dataset were analyzed with GEO2R online. Similarly, differentially expressed miRNAs from GSE70367 were also analyzed with GEO2R. The interaction between the differentially expressed miRNAs and the shared distal metastasis-related DEGs from the two datasets were analyzed using miRWalk and Cytoscape. RGS1 and miR-376-3p were chosen to verify the prediction.RGS1 stably expressing and silencing cells were established based on the MG63 and U2OS cell lines. The targeting of RGS1 with miR-376b-3p was confirmed with Starbase prediction and luciferase reporter assay.Cell proliferation, metastasis, and apoptosis were characterized in vitro and in xenograft mice.Results: A total of 10 up-regulated and 8 down-regulated DEGs were characterized as shared metastasisrelated DEGs for GSE14359 and GSE33382. Among these DEGs, RGS1 was targeted with miR-376b-3p, a predicted down-regulated miRNA in GSE70367. High expression of RGS1 predicted proliferation, invasion, metastases, and poor prognosis in osteosarcoma. Overexpression of RGS1 promoted proliferation, invasion, mobility, and stemness in MG63 and U2OS cells, while silencing of RGS1 had the opposite effect in both cell lines. High expression of RGS1 promoted tumor growth in xenograft nude mice. RGS1 was targeted with miR-376b-3p; the addition of miR-376b-3p down-regulated RGS1, and suppressed cell proliferation, invasion, and metastasis. Meanwhile, sponging of miR-376b-3p had the opposite effect. The suppressive effects of miR-376b-3p could be abolished with RGS1, as cell proliferation, stemness, metastasis, and invasion were all promoted with RGS1 co-transfection in both cell lines.Conclusions: Our study indicated that RGS1 is a tumor-promoting gene in osteosarcoma, which could be inhibited with miR-376b-3p.

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“…In addition, extracellular vesicles (EVs) released by macrophages can promote the polarization of M1-type AMs, causing the injury to the lungs ( 114 ). In contrast, the AECs-derived exosome miR-27b-3p can impact AMs functions by targeting RGS1-mediated calcium signaling-dependent immune responses ( 115 ).…”

Section: Mechanisms Of Ali Caused By Intestinal Bacterial Dysbiosismentioning

confidence: 99%

Intestinal Microbiota - An Unmissable Bridge to Severe Acute Pancreatitis-Associated Acute Lung Injury

Wang

Liu

et al. 2022

Front. Immunol.

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Severe acute pancreatitis (SAP), one of the most serious abdominal emergencies in general surgery, is characterized by acute and rapid onset as well as high mortality, which often leads to multiple organ failure (MOF). Acute lung injury (ALI), the earliest accompanied organ dysfunction, is the most common cause of death in patients following the SAP onset. The exact pathogenesis of ALI during SAP, however, remains unclear. In recent years, advances in the microbiota-gut-lung axis have led to a better understanding of SAP-associated lung injury (PALI). In addition, the bidirectional communications between intestinal microbes and the lung are becoming more apparent. This paper aims to review the mechanisms of an imbalanced intestinal microbiota contributing to the development of PALI, which is mediated by the disruption of physical, chemical, and immune barriers in the intestine, promotes bacterial translocation, and results in the activation of abnormal immune responses in severe pancreatitis. The pathogen-associated molecular patterns (PAMPs) mediated immunol mechanisms in the occurrence of PALI via binding with pattern recognition receptors (PRRs) through the microbiota-gut-lung axis are focused in this study. Moreover, the potential therapeutic strategies for alleviating PALI by regulating the composition or the function of the intestinal microbiota are discussed in this review. The aim of this study is to provide new ideas and therapeutic tools for PALI patients.

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Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response

Cited by 56 publications

References 60 publications

RETRACTED: Macrophage SAMSN1 protects against sepsis-induced acute lung injury in mice

RETRACTED: Macrophage SAMSN1 protects against sepsis-induced acute lung injury in mice

MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma

Intestinal Microbiota - An Unmissable Bridge to Severe Acute Pancreatitis-Associated Acute Lung Injury

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