Epitope Mapping for Monoclonal Antibodies Recognizing Tuber Necrotic Isolates of Potato Virus Y

Николаева, О. В.; Roop, Daniel J.; Galvino-Costa, Suellen Bárbara Ferreira; Figueira, Antônia dos Reis; Gray, Stewart M.; Karasev, Alexander V.

doi:10.1007/s12230-012-9233-8

Cited by 33 publications

(10 citation statements)

References 20 publications

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“…; Nikolaeva et al. ). This might cause confusion as a single point mutation in the recognition motifs in the CP gene would change the classification of the strains but leave the biological features of the isolates unchanged.…”

Section: Discussionmentioning

confidence: 98%

“…The occurrence of the Vietnamese isolate 8 originating from potato and Chinese isolate 1107 (accession number KC296439) originating from tobacco indicates that for a clear identification of isolates of type PVY N Wi by means of RT-PCR, it will be necessary to position strain-specific primers on the first 90 nt of the CP gene region, which is where the main linear epitopes differing between N and O strains and recognized by MAb's are located (Keller et al 2005;Chikh Ali et al 2007;Ranki et al 2008;Nikolaeva et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…There are several different MAb's with recognition motifs different from those of Bioreba's N-MAb that can be used to differentiate between NTN and Wilga isolates (Ounouna et al 2002;Chikh Ali et al 2007;Ranki et al 2008;Nikolaeva et al 2012). This might cause confusion as a single point mutation in the rec-ognition motifs in the CP gene would change the classification of the strains but leave the biological features of the isolates unchanged.…”

Section: Discussionmentioning

confidence: 99%

“…They react with the SASA O-/C-specific monoclonal antibody (MAb), but not the Bioreba N-specific MAb. The recognition sequence of this N-specific MAb is a linear epitope consisting of 17 amino acids GNDTIDAGGSTKKDAKQ (PVY N -specific amino acids underlined; Nikolaeva et al 2012). The recognition motif of the O-/C-specific MAb has yet to be determined.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

Schubert

Thieme²,

Thieme

et al. 2014

Journal of Phytopathology

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Eight isolates from different potato growing regions in Vietnam were characterized. All were highly pathogenic in some potato cultivars, but did not overcome the extreme resistance of Solanum stoloniferum and Solanum demissum. RT‐PCR analysis revealed that all of these isolates are recombinants. Sequence data for 4 isolates were obtained, and their reaction in potato cultivars harbouring specific N genes was determined. Different phylogenetic analyses of viral sequences confirmed previous results that the recombinant isolates evolved from different parental sequences. One of the Vietnamese isolates investigated had a specific structure. The need for a clear classification of PVYNWi isolates is discussed.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

Schubert

Thieme²,

Thieme

et al. 2014

Journal of Phytopathology

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“…In addition to the reference isolates, 22 PVY-infected potato leaf samples were collected from potato seed lots grown at the Othello seed lot trial field in Othello, WA, in June 2012. The infection status of tobacco and potato samples was confirmed by triple antibody sandwich enzyme-linked immunosorbent assay using a series of PVY-specific polyclonal and monoclonal antibodies as described (32).…”

Section: Methodsmentioning

confidence: 99%

An Improved Multiplex IC-RT-PCR Assay Distinguishes Nine Strains of Potato virus Y

2013

Self Cite

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A multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay was previously developed to identify a group of Potato virus Y (PVY) isolates with unusual recombinant structures (e.g., PVYNTN-NW and SYR-III) and to differentiate them from other PVY strains. In the present study, the efficiency of this multiplex RT-PCR assay was validated and extended considerably to include five additional strains and strain groups not tested before. To make the multiplex RT-PCR assay more applicable and suitable for routine virus testing and typing, it was modified by replacing the conventional RNA extraction step with the immunocapture (IC) procedure. The results obtained using well-characterized reference isolates revealed, for the first time, that this multiplex RT-PCR assay is an accurate and robust method to identify and differentiate the nine PVY strains reported to date, including PVYO (both PVYO and PVYO-O5), PVYN, PVYNA-N, PVYNTN, PVYZ, PVYE, PVY-NE11, PVYN-Wi, and PVYN:O, which is not possible by any of the previously reported RT-PCR procedures. This would make the IC-RT-PCR procedure presented here a method of choice to identify PVY strains and assess the strain composition of PVY in a given area. The IC-RT-PCR protocol was successfully applied to typing PVY isolates in potato leaf tissue collected in the field.

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Diversity, Characterisation and Classification of PVY

Glais

Lacomme

2017

Potato Virus Y: Biodiversity, Pathogenicity, Epidemiology and Management

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Epitope Mapping for Monoclonal Antibodies Recognizing Tuber Necrotic Isolates of Potato Virus Y

Cited by 33 publications

References 20 publications

Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

Molecular and Biological Characterization of Potato virus Y Isolates from Vietnam

An Improved Multiplex IC-RT-PCR Assay Distinguishes Nine Strains of Potato virus Y

Diversity, Characterisation and Classification of PVY

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