Epitope-Specific Distribution of IgG Subclasses against Antigenic Domains on Glycoproteins of Human Cytomegalovirus

Abstract

The IgG subclass pattern against linear antibody binding sites on glycoproteins of human cytomegalovirus (HCMV) was investigated in HCMV-positive healthy blood donors, human immunodeficiency virus-infected persons, sera from mothers with primary HCMV infection during pregnancy and their children, and sequential sera from transplant recipients. As antigens, three immunodominant domains capable of inducing neutralizing antibodies during natural infection were selected on glycoproteins gp58/116 (gB) and gp86 (gH). Bacterial fusion proteins representing these regions were used as antigens in a subclass-specific ELISA. Reactivity against the antibody binding site on gp86 was detected in both the IgG1 and IgG3 subclasses. In contrast, exclusively IgG1 antibodies were found against both linear domains on glycoprotein complex gp58/116 and also against full-length gp58/116 expressed in insect cells. The data demonstrate a differential regulation of the antibody response to envelope components of HCMV.

EBNA1-specific CD4+ T cells in healthy carriers of Epstein-Barr virus are primarily Th1 in function

“…The ability of IgG1 immunoglobulins to fix complement and bind to macrophage Fc receptors is thought to be important in the neutralization of free viral particles (57). We conclude that the observed polarization of EBNA1-specific IgG subclasses is most likely a consequence of a predominant Th1 phenotype of responding CD4 + lymphocytes and provides evidence for the polarization of the T-cell response to Th1 in vivo.…”

Immune monitoring of anti cytomegalovirus antibodies and risk of cytomegalovirus disease in heart transplantation

“…In addition, we observed a lack of correlation between anti-CMV titers and total IgG or IgG1 concentrations in both, D+/R− and D+/R+ CMV patients one month after HT (before development of CMV disease). It has been reported that reactivity against the antibody binding site on glycoproteins of CMV is detected in the IgG1 subclass [18], which is known to be T-cell dependent [19]. Interestingly enough, decreased baseline IgG1 concentrations were associated with higher risk of CMV disease.…”

Recombinant Modified Vaccinia Virus Ankara Expressing a Soluble Form of Glycoprotein B Causes Durable Immunity and Neutralizing Antibodies against Multiple Strains of Human Cytomegalovirus

“…gB-specific IgG 1 is the major subtype induced in response to natural HCMV infection (62). Human IgG 1 is the functional analogue of murine IgG 2a .…”