EPIYA motif patterns among Cuban Helicobacter pylori CagA positive strains

Torres, Leonel; González, Lidice; Melián, Karelia; Alonso, Jordis; Moreno, Arlenis; Hernández, Mónica López; Reyes, Orlando; Bermúdez, Ludisleydis; Campos, Javier; Pérez-Pérez, Guillermo I.; Rodrı́guez, Boris L.

doi:10.7705/biomedica.v32i1.453

Cited by 7 publications

(11 citation statements)

References 44 publications

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“…Likewise, to determine the prevalence of isolates with multiple EPIYA-C motifs in other countries, different studies have been carried-out. Fewer isolates with multiple EPIYA-C have been reported in France (32%) [22], Costa Rica (30.3%) [23], South Africa (27%) [20], Greece (21%) [24], Cuba (22.1%) [25], Mexico (20%) [26], USA (17%) [22] and Peru (11.5%) [19] in comparison with those found in this study. This might be due to the process of cagA microevolution, which describes a genetic change that leads to a modification in virulence factors, representing important implications in H. pylori pathogenesis [27,28].…”

Section: Discussioncontrasting

confidence: 51%

“…It is noteworthy that Colombia has a higher incidence of gastric cancer in comparison with other countries, possibly due in part to multiple EPIYA-C repeats. Western CagA proteins with two or more EPIYA-C repeats are more virulent and associated with a high gastric cancer risk than isolates with only one EPIYA-C motif [9, 21,25]. Moreover, the risk of gastric cancer is 12 times higher in patients infected with three EPIYA-C repeats than in patients infected with one EPIYA-C motif [13].…”

Section: Discussionmentioning

confidence: 99%

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cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

Gómez

Regino²,

Monterrey

et al. 2020

PLoS ONE

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The aim of this work was to determine current cagA gene EPIYA motifs present in Colombian Helicobacter pylori isolates using a fast and reliable molecular test. DNA from eightyfive Helicobacter pylori-cagA positive strains were analyzed. Strains were obtained from patients diagnosed with functional dyspepsia at Clínica Fundadores in Bogotá. The 3' region of the cagA gene was amplified through conventional Polymerase Chain Reaction (PCR). Obtained amplicons were sequenced using the Sanger method and analyzed with bioinformatics tools. Additionally, a significant Spearman correlation coefficient was determined between the patients' age and the number of EPIYA-C repeats; with p values < 0.05 considered significant. Estimates were obtained using a 95% CI. The 3´variable region of the cagA gene was amplified and PCR products of the following sizes corresponded to the following EPIYA motifs: 400 bp: EPIYA AB, 500 bp: EPIYA ABC, 600 bp: EPIYA ABCC and 700 bp: ABCCC. A single PCR band was observed for 58 out of 85 Helicobacter pylori isolates, with an EPIYA distribution motif as follows: 7/85 AB (8.2%), 34/85 ABC (40%), 26/85 ABCC (30.6%) and 18/85 ABCCC (21.2%). However, in 27 out of 85 Helicobacter pylori isolates, two or more bands were observed, where the most predominant cagA genotype were ABC-ABCC (26%, 7/27) and ABCC-ABCCC (22.2%, 6/27). A direct proportionality between the number of EPIYA-C repeats and an increase in the patients' age was observed, finding a greater number of EPIYA ABCC and ABCCC repeats in the population over 50 years old. All isolates were of the Western cagA type and 51.8% of them were found to have multiple EPIYA-C repeats. These standardized molecular test allowed to identify the number of EPIYA C motifs based on band size.

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Section: Discussioncontrasting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

Gómez

Regino²,

Monterrey

et al. 2020

PLoS ONE

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“…Similar to studies conducted in populations of Colombia, Cuba and Iran, in which no correlation was found between the number of EPIYA motifs and clinical outcomes, no association with the severity of histopathological changes in the gastric mucosa was found in our work [32,37,38]. In contrast, in two other Colombian studies and in Brazilian patients it was demonstrated that precancerous lesions and gastric cancer are associated with a greater number of EPIYA-C motifs [33e35].…”

Section: Discussionsupporting

confidence: 54%

“…In this regard, Rizzato et al [36] studied Venezuelan and Mexican patients with chronic gastritis (n ¼ 27) and registered 70.3%, 18.5% and 3.7% of EPIYA-ABC, ABCC and ABCCC patterns, respectively. Moreover, differences in the prevalence of strains with two and three EPIYA-C motifs in diverse gastric diseases have been reported in Cuba, Mexico and Brazil, ranging on average from 22.1% to 41.5% [31,35,37], while three different studies from Colombia reported a range of 35.8%e48.4% multiple C strains. In agreement with these studies in our samples we did not detect strains with East Asian EPIYA-D type [32e34].…”

Section: Discussionmentioning

confidence: 95%

Study of the oipA genetic diversity and EPIYA motif patterns in cagA-positive Helicobacter pylori strains from Venezuelan patients with chronic gastritis

Torres

Valderrama²,

Sayegh

et al. 2014

Microbial Pathogenesis

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“…We detected a higher significant difference between the PG and CG for the presence of multiple EPIYA repeats (Pv0. In contrast, Batista et al (2011) and Torres et al (2012) did not find any association between ulcers and the number of EPIYA-C repeats. Acosta et al (2010) and Shokrzadeh et al (2010) could not find differences between EPIYA patterns and clinical outcomes.…”

Section: Epiya-c and -D Have A Role In The Interaction Betweenmentioning

confidence: 72%

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Kocazeybek

Caliskan

Cetin

et al. 2015

Journal of Medical Microbiology

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Geographical variation in the frequency of various gastroduodenal pathologies was shown to be related to the geographical diversity of H. pylori CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) patterns. We examined the EPIYA patterns of H. pylori and the association of EPIYA patterns with gastric cancer (GC) for the first time, to the best of our knowledge, in Turkey. The patient group (PG) contained 60 patients [38 GC and 22 duodenal ulcer (DU) patients]. The control group (CG) was 110 individuals [94 gastritis patients and 16 persons with a normal gastrointestinal system (NGIS)]. Specific primers were used for the detection of cagA including empty-site-positive and EPIYA-A, -B, -C, -D PCR. Bands of EPIYA-A, -B, -C were confirmed by DNA sequencing. One hundred and forty-two (83.5 %) strains [60 in the PG (38 GC, 22 DU), 82 in the CG (72 gastritis, 10 NGIS)] were positive for the cagA gene. EPIYA-C with multiple repeats was detected in 34 (23.9 %) strains, and 22 (64.7 %) were from GC patients. EPIYA-C with one repeat was detected in 89 (62.7 %) strains, and 54 (60.7 %) were from gastritis patients. EPIYT was detected in 10 strains, and EPIYA-D was not detected. The number of EPIYA-C with multiple repeats was significantly higher for the PG than for the CG (P,0.0001). In GC patients, the number of EPIYA-C with multiple repeats was significantly higher than one repeat (P,0.0001). In conclusion, our study showed that multiple EPIYA-C repeats increases the GC risk by 30.6-fold and the DU risk by 8.9-fold versus the CG. This indicates that Western-type H. pylori strains in Turkey have similar EPIYA motifs to those of neighbouring countries and Western populations. INTRODUCTIONHelicobacter pylori is the principal cause of gastritis and gastric or duodenal ulcer (DU) diseases and is involved in the development of gastric cancer (GC) and mucosaassociated lymphoid tissue lymphoma (Atherton, 2006).H. pylori is a class 1 carcinogen identified by the International Agency for Research on Cancer for chronic and persistent infections. It is resistant to antibacterial agents in spite of innate and adaptive immunity (International Agency for Research on Cancer, 1994). Depending on virulence factors such as cytotoxin-associated gene A (CagA), blood group antigen-binding adhesion (BabA2) and vacuolating cytotoxin gene A (VacA), different genotypes cause different pathological and clinical outcomes and geographical regional differences (Atherton et al., 1995;Basso et al., 2008). Recently, the Glu-Pro-Ile-Tyr-Ala (EPIYA)Abbreviations: cagPAI, cag pathogenicity island; CG, control group; CI, confidence interval; DU, duodenal ulcer; EPIYA, Glu-Pro-Ile-Tyr-Ala; CagA, cytotoxin-associated gene A; GC, gastric cancer; NGIS, normal gastrointestinal system; PG, patient group; OR, odds ratio. pattern of CagA was suggested to be involved in the pathogenesis of GC, as this pattern manifests important variations depending on the region (Argent et al., 2004(Argent et al., , 2005.The CagA protein is injected into host epithelial cells with its peptidoglycan b...

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EPIYA motif patterns among Cuban Helicobacter pylori CagA positive strains

Cited by 7 publications

References 44 publications

cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

Study of the oipA genetic diversity and EPIYA motif patterns in cagA-positive Helicobacter pylori strains from Venezuelan patients with chronic gastritis

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

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