Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) associated with poor prognosis of head and neck carcinomas

Turunen, Aaro; Rautava, Jaana; Grénman, Reidar; Syrjänen, Kari; Syrjänen, Stina

doi:10.18632/oncotarget.16033

Cited by 29 publications

(29 citation statements)

References 27 publications

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“…Interestingly, EBER expression correlated significantly with tumor HPV positivity. The positive EBER expression in different HNSCCs (including a few OPSCC samples) has been presented recently [4]. Although the presence of HPV/EBV coinfection in OPSCC has been reported previously [39,40], we are the first to show a significant correlation between EBER positivity and HPV positivity in OPSCC in a relatively large patient cohort.…”

Section: Discussionsupporting

confidence: 49%

“…An in-house PCR and Luminex xMAP-based methods were used for EBV DNA detection as described in the previous studies [4,37]. Additionally, Epstein-Barr virus (EBV)encoded small RNA (EBER) transcripts were examined by in situ hybridization (ISH) in tumor samples to ensure the latency of EBV.…”

Section: Detection Of Ebvmentioning

confidence: 99%

“…EBER PNA Probe/Fluorescein and PNA-ISH Detection Kit (Dako, Glostrup, Denmark) were used for detecting EBV RNA transcripts EBER1 and EBER2 from the TMA slides. The methodology has been described in more detail in a previous study [4]. The substrate was first incubated then treated with eosin and finally mounted in Aquamount (Dako).…”

Section: Detection Of Ebvmentioning

confidence: 99%

“…Several viruses have been detected in head and neck squamous cell carcinoma (HNSCC) [1][2][3][4][5][6][7]. Their etiologic and prognostic role is of great interest in cancer prevention and new management practices including immunotherapy [8][9][10] and treatment de-escalation [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, patients with EBV-related NPC have a more favorable prognosis than those with EBV-negative NPC [1,22]. Furthermore, HPV has recently been found to have a prognostic impact on NPC even in non-endemic regions [1] and co-infections with EBV and HPV have also been reported [1,4,22]. In OPSCC, only a few studies have detected EBV [4,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Epstein–Barr virus (EBV) and polyomaviruses are detectable in oropharyngeal cancer and EBV may have prognostic impact

Carpén

Syrjänen

Jouhi

et al. 2020

Cancer Immunol Immunother

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Background The etiological role of human papillomavirus (HPV) in oropharyngeal squamous cell carcinoma (OPSCC) is confirmed. However, the role of other oncoviruses in OPSCC is unknown. Materials and methods A total of 158 consecutive OPSCC patients treated with curative intent were included. DNA extracted from tumor sections was used to detect Epstein-Barr virus (EBV), HPV, and the following polyomaviruses: John Cunningham virus (JCV), Simian virus 40 (SV40), and BK virus (BKV) with PCR. In addition, p16 expression was studied by immunohistochemistry, and EBV-encoded small RNA (EBER) transcripts were localized by in situ hybridization. The effect of viral status on overall survival (OS) and disease-free survival (DFS) was analyzed. Results A total of 94/158 samples (59.5%) were HPV-positive, 29.1% contained BKV DNA, 20.3% EBV DNA, 13.9% JCV DNA, and 0.6% SV40 DNA. EBER was expressed only in stromal lymphocytes adjacent to the tumor and correlated with HPV positivity (p = 0.026). p16 expression associated only with HPV. None of the three polyomaviruses had an impact on survival. Patients with EBER-positive but HPV-negative OPSCC had significantly poorer OS and DFS than those with HPVpositive OPSCC and slightly worse prognosis compared with the patients with EBER-negative and HPV-negative OPSCC. Conclusion Polyomaviruses are detectable in OPSCC but seem to have no impact on survival, whereas HPV was the strongest viral prognostic factor. EBER expression, as a sign of latent EBV infection, may have prognostic impact among patients with HPV-negative OPSCC. EBER analysis may identify a new subgroup of OPSCCs unrelated to HPV. Keywords Epstein-Barr virus • Human papillomavirus • Oropharyngeal cancer • Polyomavirus • Prognosis Abbreviations BKV BK virus DFS Disease-free survival EBER Epstein-Barr virus (EBV)-encoded small RNA * Timo Carpén

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Section: Discussionsupporting

confidence: 49%

Section: Detection Of Ebvmentioning

confidence: 99%

Section: Detection Of Ebvmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Epstein–Barr virus (EBV) and polyomaviruses are detectable in oropharyngeal cancer and EBV may have prognostic impact

Carpén

Syrjänen

Jouhi

et al. 2020

Cancer Immunol Immunother

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Low expression of pRB predicts disease relapse in early glottic cancer treated with transoral laser microsurgery

Lee¹,

Fang²,

Li³

et al. 2018

The Laryngoscope

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Objectives/Hypothesis To elucidate the associations among the immunohistochemical expression of tumor markers, clinicopathological variables, and disease‐free survival (DFS) in patients with early‐stage glottic squamous cell carcinoma (SCC) who underwent transoral laser microsurgery (TLM) as the primary treatment. Study Design Retrospective chart review. Methods The records of consecutive patients with Tis‒T2N0 glottic SCC who underwent TLM between August 1, 2012 and October 31, 2015 were reviewed. Expression of Bcl‐2, pRB, p16INK4A, p53, c‐Myc, E‐cadherin, and EGFR was examined using tissue microarrays containing tumor specimens through immunohistochemistry. Three‐year DFS rates were calculated. Results A total of 65 consecutive patients were identified, of which 28 were excluded due to insufficient tissue (n = 22) and low biomarker quality (n = 6). Therefore, 37 patients with complete records were included. The included patients were significantly older and had a more advanced type of cordectomy than did the excluded patients (P = .015 and .009, respectively). According to the findings of univariate analysis, age, betel quid chewing, type of cordectomy, BCL‐2 expression, and pRB expression significantly predicted 3‐year DFS. According to the findings of multivariate analysis, age (adjusted hazard ratio: 0.94, 95% CI: 0.88‐1.00), betel quid chewing (adjusted hazard ratio: 5.07, 95% CI: 1.32‐19.44), and pRB expression (adjusted hazard ratio: 0.02, 95% CI: 0.00‐0.28) were independent predictors of 3‐year DFS. Conclusions Low pRB expression is a potential biomarker for predicting disease relapse after primary TLM for early‐stage glottic SCC and may help to identify high‐risk patients who can subsequently undergo intensive management. Level of Evidence 4 Laryngoscope, 129:E220–E226, 2019

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A Survey of Human Papillomavirus and Epstein-Barr virus Immunohistochemical Status in Patients with Head and Neck Squamous Cell Carcinoma (HNSCC)

Javadirad

Yekta

Lorestani

et al. 2022

Head and Neck Pathol

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Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) associated with poor prognosis of head and neck carcinomas

Cited by 29 publications

References 27 publications

Epstein–Barr virus (EBV) and polyomaviruses are detectable in oropharyngeal cancer and EBV may have prognostic impact

Epstein–Barr virus (EBV) and polyomaviruses are detectable in oropharyngeal cancer and EBV may have prognostic impact

Low expression of pRB predicts disease relapse in early glottic cancer treated with transoral laser microsurgery

A Survey of Human Papillomavirus and Epstein-Barr virus Immunohistochemical Status in Patients with Head and Neck Squamous Cell Carcinoma (HNSCC)

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