“…3 The aqueous two-phase systems (ATPS) are an excellent alternative and more friendly to the environment since they are mostly composed of water and components less aggressive to nature, commercially accessible, low cost, and potentially applicable on a large scale. 4 ATPS is a versatile technique that consists of two aqueous phases and at least two other components such as polymers, 5 electrolytes, 6 ionic liquids, 7 and surfactants 8 subjected to certain conditions of temperature, pressure, and composition. Polymer/ salt/water ATPS are the most widely applied because of their low cost and rapid phase separation, which usually results in one phase being rich in polymer and poor in salt and the other phase having an inverse composition.…”