Equine Embryo Transfer

Sieme, Harald; Rau, Janina; Tiedemann, D.; Oldenhof, Harriëtte; Barros, Lawrence de Oliveira; Sanchez, Roberto; Blanco, M.; Martinsson, Gunilla; Herrera, Carolina; Burger, Dominik

doi:10.1007/978-3-319-92327-7_8

Cited by 4 publications

(10 citation statements)

References 77 publications

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“…We successfully employed a modified vitrification protocol to overcome the challenges to cryopreservation typically associated with large diameter and/or high lipid content embryos. The modifications we made to the previously published vitrification protocol (12)(13)(14) included the following: (1) replacement of glucose with galactose in the base medium, (2) replacement of fetal bovine serum with alpaca serum in all solutions containing serum, (3) use of a commercially available embryo holding medium for post-warming/pre-transfer temporary holding, (4) performing asynchronous embryo transfer (no more than 24 h asynchronous; standard operating procedure for embryo transfer on this farm) as is common in pigs (22) and horses (23), and (5) an overnight in vitro culture period to enable post-warming observation of greater duration (because embryos that initially appear viable shortly after warming often fail to produce pregnancies after embryo transfer); this enables a more accurate in vitro assessment of post-warming embryo viability.…”

Section: Discussionmentioning

confidence: 99%

Birth of a Live Cria After Transfer of a Vitrified-Warmed Alpaca (Vicugna pacos) Preimplantation Embryo

Lutz¹,

Johnson²,

Duprey³

et al. 2020

Front. Vet. Sci.

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The alpaca (Vicugna pacos) is an important species for the production of fiber and food. Genetic improvement programs for alpacas have been hindered, however, by the lack of field-practical techniques for artificial insemination and embryo transfer. In particular, successful techniques for the cryopreservation of alpaca preimplantation embryos have not been reported previously. The objective of this study was to develop a field-practical and efficacious technique for cryopreservation of alpaca preimplantation embryos using a modification of a vitrification protocol originally devised for horses and adapted for dromedary camels. Four naturally cycling non-superovulated Huacaya females serving as embryo donors were mated to males of proven fertility. Donors received 30 μg of gonadorelin at the time of breeding, and embryos were non-surgically recovered 7 days after mating. Recovered embryos (n = 4) were placed individually through a series of three vitrification solutions at 20°C (VS1: 1.4 M glycerol; VS2: 1.4 M glycerol + 3.6 M ethylene glycol; VS3: 3.4 M glycerol + 4.6 M ethylene glycol) before loading into an open-pulled straw (OPS) and plunging directly into liquid nitrogen for storage. At warming, each individual embryo was sequentially placed through warming solutions (WS1: 0.5 M galactose at 37°C; WS2: 0.25 M galactose at 20°C), and warmed embryos were incubated at 37°C in 5% CO2 in humidified air for 20–22 h in 1 ml Syngro® holding medium supplemented with 10% (v/v) alpaca serum to perform an initial in vitro assessment of post-warming viability. Embryos whose diameter increased during culture (n = 2) were transferred individually into synchronous recipients, whereas embryos that did not grow (n = 2) were transferred together into a single recipient to perform an in vivo assessment of post-warming viability. Initial pregnancy detection was performed ultrasonographically 29 days post-transfer when fetal heartbeat could be detected, and one of three recipients was pregnant (25% embryo survival rate). On November 13, 2019, the one pregnant recipient delivered what is believed to be the world's first cria produced from a vitrified-warmed alpaca embryo.

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Section: Discussionmentioning

confidence: 99%

Birth of a Live Cria After Transfer of a Vitrified-Warmed Alpaca (Vicugna pacos) Preimplantation Embryo

Lutz¹,

Johnson²,

Duprey³

et al. 2020

Front. Vet. Sci.

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“…The most appropriate day for embryo collection remains controversial. On one hand, for collections between days 6 and 9 after fertilization, collections made between days 7 and 8 retrieved greater embryo numbers (Cuervo-Arango et al, 2009;Sieme et al, 2018). On the other hand, similar embryo recovery rates could be achieved from days 5 to 8 postovulation (Vogelsang et al, 1985).…”

Section: Introductionmentioning

confidence: 92%

“…The ET makes it possible to obtain foals from aged mares, mares with problems to maintain gestation, and mares under training or competition. This biotechnology also allows obtaining foals of young mares (1 to 2 years) without impairing its development and shortens the generation interval of mares with a promising pedigree (Sieme et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The ET into previously synchronized mares can be performed by either surgical or transcervical methods (Fleury et al, 2001). Despite ongoing controversy, the most frequently used period for ET has been from days 5 to 9 after donor ovulation (Iuliano et al, 1985;Vogelsang et al, 1985;Fleury et al, 2001;Hajčić et al, 2008;Sieme et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of embryo collection and transfer days on the pregnancy rate of Mangalarga Marchador mares during the breeding season

Ferreira-Silva

Sales

Nascimento

et al. 2018

Rev Colom Cienc Pecua

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“…The reported pregnancy rate in recipient mares ranges between 65 to 85% on day-14 after ovulation [ 31 ]. The factors affecting EET programs include donor factors, embryo factors, recipient factors, male origin factors, and technical factors [ 32 ]. Donor mares’ fertility is affected by age, breed, intrinsic fertility, parity, sports activity, nutritional status, lactational status, and other reproductive pathologies [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the efficiency of equine embryo transfer (EET) in polo mares under subtropical conditions of Pakistan

Mahmood,

Ali Channa,

Ghafoor

et al. 2024

PLoS ONE

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Equine embryo transfer (EET) is a prominent technology in the equine breeding industry, and its efficacy is affected by a number of factors. The current study aimed to determine the effects of the breed of donor/recipient mares, estrus/ovulation induction treatment, cooled transportation of embryos, and synchrony between donor and recipient mares on the efficiency of the EET under subtropical conditions of Pakistan. A total of eighty-four (n = 84) Polo-playing donor mares (Argentino-polo = 41 and Anglo-Arab = 43) and seventy (n = 70) recipient mares (light breed = 26 and heavy breed = 44) were used for EET. The donor mares exhibiting natural estrus (n = 28) were detected by teaser a stallion, and corpus luteum (CL) having mares (n = 56) were treated with prostaglandin (150 μg of Cloprostenol) for estrus induction. The mares’ follicular growth was monitored through ultrasonography until the dominant follicle’s size reached 35 mm or more with a moderate to obvious uterine edema score. Afterward, the mares were treated either with GnRH, i.e., 50 μg of Lecirelin acetate (n = 41) or Ovusyn, i.e., 1500 IU hCG (n = 43). Insemination with chilled semen was performed 24 hours later. The embryos were collected non-surgically, 7 or 8 days after ovulation, from the donor mares. The collected embryos were transferred into the well-synchronized recipient mares as fresh (n = 44) or chilled (n = 26) embryos. The pregnancy after ET was checked through ultrasonography. Statistical analysis revealed that the embryo recovery rate (ERR) remained significantly higher (P<0.05) for the Prostaglandin (PG) treated group of donors as compared to the natural heat group of donors. The breed of donor mares, type of ovulatory treatment given, and day of embryo collection did not significantly (P>0.05) affect the ERR. There was no significant effect of the type (fresh vs chilled), classification, and stage of development of embryo on pregnancy outcomes (P>0.05). ET pregnancy rate was significantly affected by the breed of recipient mares and ovulation synchrony between donor and recipient mares (P<0.05). In conclusion, under the subtropical conditions of Pakistan, PG-based estrus induction of donor mares, breed of recipient mares, and ovulation synchrony between the donor and recipient mares had a substantial effect on the efficiency of EET.

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Equine Embryo Transfer

Cited by 4 publications

References 77 publications

Birth of a Live Cria After Transfer of a Vitrified-Warmed Alpaca (Vicugna pacos) Preimplantation Embryo

Birth of a Live Cria After Transfer of a Vitrified-Warmed Alpaca (Vicugna pacos) Preimplantation Embryo

Evaluation of embryo collection and transfer days on the pregnancy rate of Mangalarga Marchador mares during the breeding season

Factors affecting the efficiency of equine embryo transfer (EET) in polo mares under subtropical conditions of Pakistan

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