Equine Gastric Squamous Cell Carcinoma in a Friesian Stallion

Straticò, Paola; Razzuoli, Elisabetta; Hattab, Jasmine; Celani, Gianluca; Palozzo, Adriana; Bonanni, Daniele; Fruscione, Floriana; Varasano, Vincenzo; Petrizzi, Lucio; Marruchella, Giuseppe

doi:10.1016/j.jevs.2022.104087

Cited by 5 publications

(6 citation statements)

References 39 publications

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“…In veterinary literature, the association of equine vulvar SCCs with PVs has been reported in few cases [22], but the real prevalence is still unknown. However, to the authors' best knowledge, all published cases were positive to EcPV2 [21,36,44,[47][48][49]. In a previously described clinical case involving an Italian pony, multifocal areas of squamous epithelial hyperplasia associated with moderate nucleocytoplasmic atypia, together with an in situ carcinoma (vulvar intraepithelial lesion, VIN), were considered precursors of SCC, as hypothesized in women [36].…”

Section: Discussionmentioning

confidence: 92%

Equus caballus Papillomavirus Type-9 (EcPV9): First Detection in Asymptomatic Italian Horses

Paolis

Ciucis

Peletto

et al. 2022

Viruses

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Papillomavirus (PV) infections may be related to anogenital lesions and cancer development in humans and several other animal species. To date, 11 different PVs have been reported in horses. Among them, a newly described PV named Equus caballus Papillomavirus Type9 (EcPV9) was thus far only reported in the semen of a stallion with penile lesions in Australia. This study reports for the first time the presence of EcPV9 in asymptomatic Italian horses. From July 2020 to January 2022, genital brush samples were collected from 209 horses with no apparent signs of neoplastic disease and no PV-associated lesions, clinically examined at the Didactic Veterinary University Hospital (OVUD) of Perugia and at the Veterinary University Hospital (OVU) of Turin. Brushes were submitted to real-time PCR targeting the EcPV9-L1 region. The first amplification targeted a region of ~116 bp, followed by the amplification and sequencing of ~533 bp of the positive samples. EcPV9-L1 DNA was found in eleven horses (5.3%), all female and mainly English Thoroughbred. Co-infection with EcPV2-L1 was found in 7 out of the 11 EcPV9-L1 positive horses (63.6%). This study contributes to the description of the prevalence of exposure or infection of EcPVs in the horse population in Italy, for which data are still limited. In this regard, here we provide a phylogenetic analysis and the completely reconstructed viral genomes of two Italian EcPV type 9 isolates, as well as four EcPV type 2 obtained from co-infected animals.

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Section: Discussionmentioning

confidence: 92%

Equus caballus Papillomavirus Type-9 (EcPV9): First Detection in Asymptomatic Italian Horses

Paolis

Ciucis

Peletto

et al. 2022

Viruses

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“…Likewise, PVs have been associated with malignant neoplasms in several animal species [ 11 ]. In horses, a growing body of evidence suggests that EcPV2 infection contributes to the etiopathogenesis of squamous cell carcinomas, which can affect the external genitalia, and laryngeal and gastric mucosa [ 14 , 15 ]. Other types of equine papillomaviruses have been associated with different lesions.…”

Section: Discussionmentioning

confidence: 99%

Bovine Papillomavirus Type 1 Infection in an Equine Congenital Papilloma

Maggi,

De Paolis,

De Santis

et al. 2023

Pathogens

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Papillomas are benign epithelial lesions protruding on the epithelial surfaces as finger-like or warty projections. These lesions are often caused by papillomavirus (PV) infection. Congenital papillomas have been reported in foals. However, to date, no evidence of PV infection has been provided. In the present paper, we describe the main clinical–pathological features of a congenital papilloma observed in a foal. In addition, biomolecular tests demonstrated BPV1 infection in the case under study. Such data stimulate further investigations, even on archived samples, aiming to clarifying the etiology of equine congenital papilloma and the clinical relevance, if any, of BPV1 vertical transmission in horses.

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“…Additionally, EcPV2 E6/E7 nucleic acids were detected in equine gastric SCCs, of which 7/11 (64%) and 5/11 (45%) were positive with PCR and ISH, respectively (Alloway et al, 2020). There have been other recent case reports of horses with EcPV2-associated gastric SCC (Porcellato et al, 2020;Straticò et al, 2022).…”

Section: Introductionmentioning

confidence: 95%

Equus caballus papillomavirus type 2 (EcPV2)‐associated benign penile lesions and squamous cell carcinomas

Tuomisto,

Virtanen,

Kegler

et al. 2024

Veterinary Medicine & Sci

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BackgroundSquamous cell carcinoma is the most common genital, ocular and gastric tumour in horses. Equus caballus papillomavirus type 2 (EcPV2) DNA has been detected in several studies in equine penile squamous cell carcinomas (SCCs) and precursor lesions providing evidence of a causal role of EcPV2 in equine genital SCCs. Recently, EcPV2 E6/E7 nucleic acids were also detected in equine gastric SCCs, but further studies are required to determine the role of EcPV2 infection in the pathogenesis of gastric SCC. EcPV2 nucleic acids have been rarely described in ocular SCCs and precursor lesions.ObjectivesTo investigate the presence of EcPV2 nucleic acids with polymerase chain reaction (PCR) and in situ hybridisation (ISH) in penile hyperplasias, papillomas and SCCs in horses and to determine whether EcPV2 nucleic acids can be detected in SCCs affecting other locations, including the stomach, ocular tissues and larynx.MethodsTwenty‐one archival formalin‐fixed paraffin embedded (FFPE) tissue samples, including 12 genital lesions comprising penile hyperplasias, papillomas and SCCs, 6 ocular SCCs, 2 gastric SCCs and 1 laryngeal SCC, were screened by PCR and ISH for EcPV2 E6/E7 DNA and mRNA. Archival FFPE tissue samples (eyelid and penile mucosa and preputium) from six horses without a diagnosis or history of neoplastic or papillomavirus‐associated disease were included as controls.ResultsEcPV2 nucleic acids were detected by PCR and ISH in all genital lesions (12/12) and gastric SCCs (2/2), in two ocular SCCs (2/6) and in one laryngeal SCC (1/1). In control horses, one eyelid sample was positive in PCR but not in ISH. The remaining control samples were negative for EcPV2 E6/E7 nucleic acids in PCR and ISH.ConclusionsThese results further support the role of EcPV2 infection in the development of equine genital SCCs and suggest that EcPV2 infection may also act as a predisposing factor for other SCCs in horses, including gastric, ocular and laryngeal SCCs.

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Equine Gastric Squamous Cell Carcinoma in a Friesian Stallion

Cited by 5 publications

References 39 publications

Equus caballus Papillomavirus Type-9 (EcPV9): First Detection in Asymptomatic Italian Horses

Equus caballus Papillomavirus Type-9 (EcPV9): First Detection in Asymptomatic Italian Horses

Bovine Papillomavirus Type 1 Infection in an Equine Congenital Papilloma

Equus caballus papillomavirus type 2 (EcPV2)‐associated benign penile lesions and squamous cell carcinomas

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