Equine seminal plasma and sperm membrane: Functional proteomic assessment

Guasti, Priscilla Nascimento; Souza, Fabiana Ferreira de; Scott, Caroline; Papa, P.M.; Camargo, Laíza Sartori de; Schmith, Rúbia Alves; Monteiro, Gabriel Augusto; Hartwig, F.P.; Papa, Federico

doi:10.1016/j.theriogenology.2020.06.014

Cited by 19 publications

(16 citation statements)

References 65 publications

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“…The first SDS-PAGE analysis of stallion SP proteins was performed by Amann et al (1987) who identified 27 PFs with a molecular weight of 13.0 to 122.0 kDa. The most abundant proteins identified in 2D PAGE maps and complex protein mixtures included 24 proteins from stallion SP (Guasti et al, 2020). In the present study, approximately 21 PFs with a molecular weight of 8.7 to 130.6 kDa were identified as ZnBPs in stallion SP.…”

Section: Discussionsupporting

confidence: 45%

Zinc-binding proteins in stallion seminal plasma as potential sperm function regulators

Mogielnicka-Brzozowska

Piątkowska

Fraser

et al. 2022

Annals of Animal Science

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Sperm functions may be influenced by seminal plasma (SP) proteins with affinity to zinc ions. The aim of the study was to isolate and characterise zinc-binding proteins (ZnBPs) from stallion SP using proteomic tools. Zinc-binding proteins were isolated from the SP of eight stallions by zinc-affinity chromatography. They were analysed in the SDS-PAGE system, and peptide extracts were prepared. Samples of ZnBPs isolated from stallion SP were injected onto the nLC-1000 nanoflow HPLC system coupled via a nano-electrospray ion source to the Orbitrap Elite FTMS mass spectrometer (Thermo Fisher Scientific). Raw MS data were analysed using MaxQuant software with label-free quantification (LFQ). Protein sequences were obtained from the UniProt database. Forty-seven proteins in ZnBPs were annotated in a gene ontology (GO) analysis. The LFQ intensity analysis of individual proteins revealed that ZnBPs comprised mainly clusterin (CLU, 27%), serin protease inhibitor F1 (SERPINF1, 13%), actin, cytoplasmic 1 (ACTB, 9%), nucleobindin 2 (NUCB2, 8%) and polymeric immunoglobulin receptor (PIGR, 6%). This is the first proteomic study to analyse ZnBPs in stallion SP. The present findings show that ZnBPs in stallion SP could play an important role in the regulation of sperm function.

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Section: Discussionsupporting

confidence: 45%

Zinc-binding proteins in stallion seminal plasma as potential sperm function regulators

Mogielnicka-Brzozowska

Piątkowska

Fraser

et al. 2022

Annals of Animal Science

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“…Research into other Izumo proteins suggests their involvement in fertilization. Izumo1-3 are transmembrane testis expressed proteins ( Ellerman et al, 2009 ), while Izumo4 lacks a transmembrane domain and is expressed in the acrosome ( Guasti et al, 2020 ). Izumo3 shows evidence of positive selection ( Grayson and Civetta, 2012 ), and is necessary for sperm acrosome formation ( Inoue et al, 2021b ).…”

Section: Izumo/junomentioning

confidence: 99%

The Importance of Gene Duplication and Domain Repeat Expansion for the Function and Evolution of Fertilization Proteins

Rivera

Swanson

2022

Front. Cell Dev. Biol.

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The process of gene duplication followed by gene loss or evolution of new functions has been studied extensively, yet the role gene duplication plays in the function and evolution of fertilization proteins is underappreciated. Gene duplication is observed in many fertilization protein families including Izumo, DCST, ZP, and the TFP superfamily. Molecules mediating fertilization are part of larger gene families expressed in a variety of tissues, but gene duplication followed by structural modifications has often facilitated their cooption into a fertilization function. Repeat expansions of functional domains within a gene also provide opportunities for the evolution of novel fertilization protein. ZP proteins with domain repeat expansions are linked to species-specificity in fertilization and TFP proteins that experienced domain duplications were coopted into a novel sperm function. This review outlines the importance of gene duplications and repeat domain expansions in the evolution of fertilization proteins.

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“…Following spermatogenesis, spermatozoa are exposed to a variety of proteinic and non-proteinic substances secreted by the accessory sex glands which aid in the acquisition of mature male fertility and sperm survival during transportation through the male tract and into the female tract [ 13 , 14 , 15 , 34 ]. However, the remainder of this review will be focusing on the mature ejaculated spermatozoa and its relation to IVP, a process during which seminal plasma is largely removed, and, thus, we will not be elaborating on the significance of accessory sex glands and their secretions.…”

Section: Spermatogenesismentioning

confidence: 99%

“…Studies of equine sperm fertility encompass sperm biogenesis [ 3 , 4 ], motility and metabolism [ 5 , 6 ], morphology [ 7 ], sperm ultrastructure [ 8 ], and biochemical elements of sperm function [ 9 , 10 , 11 , 12 ], including sperm interactions with accessory sex gland secretions [ 13 , 14 , 15 ]. The wholistic picture of sperm fertility is integral to the maximization of IVP outcomes, and, therefore, in Section I of this review we will focus on equine spermatogenesis, sperm morphology, and common sperm abnormalities leading to infertility.…”

Section: Introductionmentioning

confidence: 99%

An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section I

Orsolini

Meyers

Dini

2021

Animals

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As the use of assisted reproductive technologies (ART) and in vitro embryo production (IVP) expand in the equine industry, it has become necessary to further our understanding of semen physiology as it applies to overall fertility. This segment of our two-section review will focus on normal sperm parameters, beginning with development and extending through the basic morphology of mature spermatozoa, as well as common issues with male factor infertility in IVP. Ultimately, the relevance of sperm parameters to overall male factor fertility in equine IVP will be assessed.

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Equine seminal plasma and sperm membrane: Functional proteomic assessment

Cited by 19 publications

References 65 publications

Zinc-binding proteins in stallion seminal plasma as potential sperm function regulators

Zinc-binding proteins in stallion seminal plasma as potential sperm function regulators

The Importance of Gene Duplication and Domain Repeat Expansion for the Function and Evolution of Fertilization Proteins

An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section I

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