Equipment for multifunctional use in high-performance capillary electrophoresis

Electrophoretic separations on chips with hydrodynamically closed separation systems

“…The use of the closed separation system with the driving electrodes placed outside the chip channels, as employed in some application-related works [45][46][47], although eliminating impacts of the products of the electrode reactions on the migration velocities, does not eliminate HDF due to gas formation. This source of HDF can be eliminated in the way as employed in some conventional CE equipment for ITP [17,18,48] and CZE [31][32][33][34]49] separations. Here, semipermeable membranes (see also Figs.…”

Calculation of the composition of sample zones in capillary zone electrophoresis IV. Weak acids and system characteristics

“…8 where the BGEs consisted of a mixture of 0.02 M histidine and (a) 0.01 M hydrochloric acid, (b) 0.01 M butyric acid and (c) 0.005 M hydrochloric acid and 0.005 M butyric acid. The electropherograms were measured in the anionic mode (anode at the outlet, applied voltage 10 kV) and, in order to suppress the EOF, 0.025% mowiol poly(vinyl alcohol) (PVA) and 5.10 4 M cetyltrimethylammoniumhydroxide (CTA) were added to all BGEs [11]. Using the single co-ion, chloride (electropherogram a), with high mobility, the first sample peak nitrate is sharp, whereas sample peaks of components with lower mobilities show peak- broadening due to the increasingly electrodispersive effect.…”

Mould‐devouring mites differ in guanine excretion from dust‐eating Acari, a possible error source in mite allergen exposure studies

“…Guanine was quantitatively assessed in the collected dust by means of capillary zone electrophoresis (CZE) [20,21] (10 mM Tartaric acid, i3-Alanine, pH3; capillary of 50 cm in length, internal diameter 50 /im, 30 kV, detection at 254 nm). A Beckman p/ace system 2000 (Beckman Instruments, California, USA) was used.…”