Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles

Silva, Talyne Emilia Santos; Brito, Danielle Cristina Calado de; Sá, Naíza Arcângela Ribeiro de; Silva, Renato Félix da; Ferreira, Anna Clara Accioly; Silva, José Ytalo Gomes da; Guedes, Maria Izabel Florindo; Rodrigues, Ana Paula Ribeiro; Santos, Regiane R.; Figueiredo, J.R.

doi:10.3390/toxins11110652

Cited by 7 publications

(4 citation statements)

References 65 publications

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“…The development of antral follicles in gilts born from sows exposed to 0.2 or 0.5 mg/kg ZEN via the diet was not affected, whereas apoptosis and cytoplasm vacuolization was observed in the oocytes enclosed in preantral follicles [193]. Similar signs of degeneration were observed in oocytes from sheep preantral follicles exposed to ZEN in vitro, where ZEN impaired the proliferation of granulosa cells from primary and secondary preantral follicles and caused DNA double-strain breaks in the oocytes from primordial follicles [194,195]. The presence of mycotoxins in the follicular fluid cannot be excluded.…”

Section: In Vitro Experimental Trialsmentioning

confidence: 76%

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Gallo

Mosconi

Trevisi

et al. 2022

Dairy

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With an increased knowledge of the mechanism of action of Fusarium mycotoxins, the concept that these substances are deleterious only for monogastric species is obsolete. Indeed, most mycotoxins can be converted into less toxic compounds by the rumen microflora from healthy animals. However, mycotoxin absorption and its conversion to more toxic metabolites, as well as their impact on the immune response and subsequently animal welfare, reproductive function, and milk quality during chronic exposure should not be neglected. Among the Fusarium mycotoxins, the most studied are deoxynivalenol (DON), zearalenone (ZEN), and fumonisins from the B class (FBs). It is remarkable that there is a paucity of in vivo research, with a low number of studies on nutrient digestibility and rumen function. Most of the in vitro studies are related to the reproductive function or are restricted to rumen incubation. When evaluating the production performance, milk yield is used as an evaluated parameter, but its quality for cheese production is often overlooked. In the present review, we summarize the most recent findings regarding the adverse effects of these mycotoxins with special attention to dairy cattle.

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Section: In Vitro Experimental Trialsmentioning

confidence: 76%

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Gallo

Mosconi

Trevisi

et al. 2022

Dairy

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“…In conclusion, we demonstrated the effects of two xenoestrogens on the morphology of sheep preantral follicles. As showed in previous studies, ZEN impairs the morphology and developmental capacity of preantral follicles (Schoevers et al, 2012;Silva et al, 2019). This oestrogenic mycotoxin is known by its capacity to induce oxidative stress and to decrease the antioxidant capacity of granulosa cells (Qin et al, 2015).…”

Section: Resultsmentioning

confidence: 71%

“…All supplements were used at a concentration of 1 μmol/l. Concentrations of the test compounds were selected based on previously tested levels of ZEN in combination with the xenoestrogen equivalent (1 μmol/l) (Silva et al, 2019). After in vitro culture, ovarian fragments were fixed for histological analysis.…”

Section: Experimental Designmentioning

confidence: 99%

Effects of in vitro exposure of sheep ovarian tissue to zearalenone and matairesinol on preantral follicles

Brito

Silva

Ferreira

et al. 2021

Zygote

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Summary The aim of this study was to evaluate the effect of 1 µmol/l zearalenone (ZEN) and 1 µmol/l matairesinol (MAT), alone or in combination, on the morphology of in vitro-cultured ovarian preantral follicles. Ovaries from four adult sheep were collected at a local slaughterhouse and fragmented, and the ovarian pieces were submitted to in vitro culture for 3 days in the presence or absence of the test compounds. The morphology of primordial and primary follicles was impaired by ZEN. The plant lignan MAT alone did not maintain the morphology of the ovarian follicles; its combination with ZEN counteracted the negative effects observed when follicles were cultured in the presence of the mycotoxin alone. However, MAT was not able to promote the in vitro development of the ovarian follicles.

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“…Earlier studies indicate that the impacts on oocyte maturation occurred in the absence of degeneration and DNA damage [ 83 ], consistent with the evidence that ZEN is a potent estrogen, but not overtly cytotoxic [ 74 ]. However, more recent research shows that ZEN exposure leads to an increase in DNA double-strand breaks (DSBs) in ovine and murine models [ 24 , 84 ]. At lower doses (0.001 to 3.1 µM), administration of ZEN or its metabolite α-ZOL stimulated proliferation of bovine and mare primary granulosa cells [ 85 , 86 ].…”

Section: Resultsmentioning

confidence: 99%

Impact of Fusarium-Derived Mycoestrogens on Female Reproduction: A Systematic Review

Kinkade

Rivera‐Núñez

Gorcyzca

et al. 2021

Toxins

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Contamination of the world’s food supply and animal feed with mycotoxins is a growing concern as global temperatures rise and promote the growth of fungus. Zearalenone (ZEN), an estrogenic mycotoxin produced by Fusarium fungi, is a common contaminant of cereal grains and has also been detected at lower levels in meat, milk, and spices. ZEN’s synthetic derivative, zeranol, is used as a growth promoter in United States (US) and Canadian beef production. Experimental research suggests that ZEN and zeranol disrupt the endocrine and reproductive systems, leading to infertility, polycystic ovarian syndrome-like phenotypes, pregnancy loss, and low birth weight. With widespread human dietary exposure and growing experimental evidence of endocrine-disrupting properties, a comprehensive review of the impact of ZEN, zeranol, and their metabolites on the female reproductive system is warranted. The objective of this systematic review was to summarize the in vitro, in vivo, and epidemiological literature and evaluate the potential impact of ZEN, zeranol, and their metabolites (commonly referred to as mycoestrogens) on female reproductive outcomes. We conducted a systematic review (PROSPERO registration CRD42020166469) of the literature (2000–2020) following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The data sources were primary literature published in English obtained from searching PubMed, Web of Science, and Scopus. The ToxR tool was applied to assess risk of bias. In vitro and in vivo studies (n = 104) were identified and, overall, evidence consistently supported adverse effects of mycoestrogens on physiological processes, organs, and tissues associated with female reproduction. In non-pregnant animals, mycoestrogens alter follicular profiles in the ovary, disrupt estrus cycling, and increase myometrium thickness. Furthermore, during pregnancy, mycoestrogen exposure contributes to placental hemorrhage, stillbirth, and impaired fetal growth. No epidemiological studies fitting the inclusion criteria were identified.

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Equol: A Microbiota Metabolite Able to Alleviate the Negative Effects of Zearalenone during In Vitro Culture of Ovine Preantral Follicles

Cited by 7 publications

References 65 publications

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Effects of in vitro exposure of sheep ovarian tissue to zearalenone and matairesinol on preantral follicles

Impact of Fusarium-Derived Mycoestrogens on Female Reproduction: A Systematic Review

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